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首页> 外文期刊>Biomaterials Science >In vitro study of the host responses to model biomaterials via a fibroblast/macrophage co-culture system
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In vitro study of the host responses to model biomaterials via a fibroblast/macrophage co-culture system

机译：通过成纤维细胞/巨噬细胞共培养系统对模型生物材料进行模型生物材料的体外研究

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Surface properties are believed to play important roles in initial inflammatory and subsequent wound healing/fibrotic responses after implantation of biomaterials. To investigate the surface property effect in mediating these host responses, we used an in vitro fibroblast/macrophage co-culture model established with a cell migration chamber, and a series of self-assembling monolayers (SAMs) bearing different terminal groups as model surfaces to study the effect of surface properties on macrophage fusion, fibroblast attachment, spreading morphology, proliferation, outgrowth, as well as pro-(interleukin- 6) and anti-(interleukin-10) inflammatory cytokine production, expression of ED-A fibronectin (FN) and alpha-smooth muscle actin (alpha-SMA). The obtained results show that the hydrophobic CH3 surface caused high levels of inflammatory but low levels of wound healing/fibrotic responses, while the hydrophilic/anionic COOH surface resulted in both low levels of inflammatory and wound healing/fibrotic responses. Interestingly, the hydrophilic OH surface was found to possess a low potential of inducing inflammatory responses but high potential of inducing wound healing/fibrotic responses. These results reveal that the extent of inflammation and wound healing/fibrosis might not be always related in vitro. However, more important is the observation of the macrophage contributions in facilitating the wound healing and fibrotic responses by up-regulation of fibroblast outgrowth, cytokine production as well as ED-A FN and alpha-SMA expression. Overall, by linking the surface properties to cell activities with our established fibroblast/macrophage co-culture system, we could provide an useful model system for in vitro studies to design more biocompatible biomaterials for various biomedical and tissue engineering applications.
机译：据信表面性质在植入生物材料植入后在初始炎症和随后的伤口愈合/纤维化反应中起重要作用。为了研究在介导这些宿主反应中的表面性质效应，我们使用与细胞迁移室建立的体外成纤维细胞/巨噬细胞共培养模型，以及轴承不同终端组的一系列自组装单层（SAMS）作为模型表面研究表面性质对巨噬细胞融合，成纤维细胞附着，扩散形态，增殖，产物的影响，以及抗（白细胞介素-10）炎症细胞因子产生，ED-A纤连蛋白的表达（FN ）和α-平滑肌肌动蛋白（alpha-sma）。得到的结果表明，疏水性CH3表面引起了高水平的炎症但低水平的伤口愈合/纤维化反应，而亲水/阴离子CoOH表面导致炎症和伤口愈合/纤维化反应的低水平。有趣的是，亲水性OH表面被发现具有诱导炎症反应的低潜力，但诱导伤口愈合/纤维化反应的高潜力。这些结果表明，炎症和伤口愈合/纤维化的程度可能并不总是在体外相关的。然而，更重要的是观察巨噬细胞促进伤口愈合和纤维化反应，通过升高成纤维细胞产高，细胞因子产生以及ED-A Fn和α-SMA表达。总体而言，通过将表面特性与我们已建立的成纤维细胞/巨噬细胞共同培养系统连接到细胞活性，我们可以为体外研究提供有用的模型系统，以设计更多生物医学和组织工程应用的更生物相容性生物材料。

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  • 来源
    《Biomaterials Science》 |2017年第1期|共12页
  • 作者

    Zhou Guoying; Liedmann Andrea; Chatterjee Chandralekha; Groth Thomas;

  • 作者单位

    Martin Luther Univ Halle Wittenberg Biomed Mat Grp Inst Pharm Heinrich Damerow Str 4 D-06120 Halle Saale Germany;

    Martin Luther Univ Halle Wittenberg Biomed Mat Grp Inst Pharm Heinrich Damerow Str 4 D-06120 Halle Saale Germany;

    Martin Luther Univ Halle Wittenberg Biomed Mat Grp Inst Pharm Heinrich Damerow Str 4 D-06120 Halle Saale Germany;

    Martin Luther Univ Halle Wittenberg Biomed Mat Grp Inst Pharm Heinrich Damerow Str 4 D-06120 Halle Saale Germany;

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  • 正文语种 eng
  • 中图分类 分子生物学;
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