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首页> 外文期刊>Biomaterials Science >Para-substituted sulfonic acid-doped protonated emeraldine salt nanobuds: a potent neural interface targeting PC12 cell interactions and promotes neuronal cell differentiation
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Para-substituted sulfonic acid-doped protonated emeraldine salt nanobuds: a potent neural interface targeting PC12 cell interactions and promotes neuronal cell differentiation

机译：对替代的磺酸掺杂质子化蛋白质盐纳米：靶向PC12细胞相互作用并促进神经元细胞分化的有效神经界面

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摘要

Structural parameters, such as metal-like semiconductor and electrochemical properties of functionalized polyaniline, hold great potential especially for the development of the cell-substrate interface due to its ion/electron transfer ability. We report the one-step synthesis of sulfonic acid-doped polyaniline nanobuds (s-PANINbs) with controlled shape/size under various oxidation potentials. The different oxidation states of s-PANINbs are used to investigate the cell-specific platform for the induction of neuronal networks in PC12 cells, including the growth, proliferation, and differentiation of cells. The unique structure of one-dimensional (1-D) s-PANINbs enhances its intrinsic conductive properties, and facilitates the dispersibility and electrochemical activity via covalent bonding with dopants. The protonated emeraldine salt nanobuds of s-PANINbs synthesized at 0.18 V anodic potential demonstrated low resistivity (～81.18 mΩ) and charge transfer resistance (～3253 Ω). The most biologically compatible protonated emeraldine salt was used in vitro to induce PC12 cells associated with neurite outgrowth, contributing to the electrophysiology of neuronal cells under an external electrical stimulation. The western blotting analysis and qRT-PCR results show that β-III Tubulin, synapsin I, and TREK-1 are highly expressed in PC12 cells, confirming their successful differentiation into neural-specific cells. Our approach demonstrates the promising role of the self-standing framework based on the s-PANINbs of the protonated emeraldine salt in peripheral nerve repair for the future in vivo cell-interface.
机译：诸如金属状半导体和官能化聚苯胺的电化学性能，例如由于其离子/电子转移能力，特别是用于开发电池 - 基板界面的巨大潜力。我们在各种氧化潜力下报道了具有受控形状/尺寸的磺酸掺杂聚苯胺纳米管（S-Paninbs）的一步合成。 S-Paninbs的不同氧化态用于探讨用于诱导PC12细胞中神经元网络的细胞特异性平台，包括细胞的生长，增殖和分化。一维（1-D）S-Paninbs的独特结构增强了其内在导电性能，并通过与掺杂剂共价键合的分散性和电化学活性。在0.18 V阳极电位的S-Paninbs的质子化牛肉盐纳米在电阻下进行了低电阻率（〜81.18mΩ）和电荷转移电阻（〜3253Ω）。在体外使用最具生物相容的质子化欧洲盐，诱导与神经突沟相关的PC12细胞，有助于在外部电刺激下的神经元细胞的电生理学。蛋白质印迹分析和QRT-PCR结果表明，β-III管蛋白，Synappain I和Trek-1在PC12细胞中高度表达，证实它们成功分化为神经特异性细胞。我们的方法展示了自我支持框架的有希望的基于体内神经修复中的质子祖美盐的S-Paninbs在体内细胞界面的未来。

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  • 来源
    《Biomaterials Science》 |2021年第5期|共14页
  • 作者

    Sita Shrestha; Bishnu Kumar Shrestha; Oh Kwang Joong; Chan Hee Park; Cheol Sang Kim;

  • 作者单位

    Department of Bionanotechnology and Bioconvergence Engineering Graduate School Jeonbuk National University Jeonju Republic of Korea;

    Department of Bionanotechnology and Bioconvergence Engineering Graduate School Jeonbuk National University Jeonju Republic of Korea;

    Department of chemistry Jeonbuk National University Jeonju 561-756 Republic of Korea;

    Department of Bionanotechnology and Bioconvergence Engineering Graduate School Jeonbuk National University Jeonju Republic of Korea;

    Department of Bionanotechnology and Bioconvergence Engineering Graduate School Jeonbuk National University Jeonju Republic of Korea;

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  • 正文语种 eng
  • 中图分类 计量学;
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