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首页> 外文期刊>Biomaterials Science >Micro/nano-net guides M2-pattern macrophage cytoskeleton distribution via Src-ROCK signalling for enhanced angiogenesis
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Micro/nano-net guides M2-pattern macrophage cytoskeleton distribution via Src-ROCK signalling for enhanced angiogenesis

机译：微/纳米净引导M2-模式巨噬细胞骨骼骨骼骨骼分布通过SRC岩石信号进行增强血管生成

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摘要

Implant surface topography has been proven to determine the fate of adhered macrophage polarization, which is closely related to the cytoskeletal arrangement during adhesion. Our purpose was to establish a topography that is favourable to M2 macrophage switching by regulating macrophage cytoskeleton distribution. Two micro/nano-net structures with different pore sizes were generated by alkali bathing at medium (SAM) or high (SAH) temperature based on the micro-level surface. Their surface characteristics, in vitro macrophage polarization and impact on endothelial cells were analysed. The in vivo macrophage response and osseointegration were also tested. The results showed that the micro/nano-net has high hydrophilicity and moderate roughness. In the SAH and SAM groups, macrophages exhibited an elongated cytoskeleton with tiny protrusions and had a high M2/M1 polarization ratio with enhanced angiogenic ability, and in vivo studies also showed faster angiogenesis and bone formation in these groups. SAH showed even better results than SAM. For cytoskeleton related pathway explanation, ROCK expression was upregulated and Src expression was downregulated at the early or late adhesion stage in both the SAH and SAM groups. These results indicated that the micro/nano-net structure guides elongated macrophage adhesion states via Src-ROCK signalling and switches macrophages towards the M2 phenotype, which provides a cytoskeleton-oriented topography design for an ideal immune response.
机译：已证明植入表面形貌以确定粘附巨噬细胞极化的命运，其与粘附期间的细胞骨骼布置密切相关。我们的目的是通过调节巨噬细胞骨骼分布来建立一种有利于M2巨噬细胞切换的地形。基于微水平表面，通过在培养基（SAM）或高（SAH）温度下的碱沐浴产生具有不同孔径的微/纳米净结构。分析了它们的表面特性，体外巨噬细胞极化和对内皮细胞的影响。还测试了体内巨噬细胞反应和骨整合。结果表明，微/纳米净具有高亲水性和中等粗糙度。在SAH和SAM组中，巨噬细胞呈细长的细胞骨架，具有微小突起，具有高的M2 / M1偏振率，具有增强的血管生成能力，并且体内研究也显示出这些组中的更快的血管生成和骨形成。 SAH表现出比SAM更好的结果。对于细胞骨架相关途径说明，岩体表达上调，SRC表达在SAH和SAM组的早期或晚期粘附阶段下调。这些结果表明，微/纳米净结构通过SRC岩石信号传导引导细长的巨噬细胞粘附状态，并将巨噬细胞朝向M2表型切换，这为理想的免疫应答提供了一种胞骨架导向的地形设计。

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  • 来源
    《Biomaterials Science》 |2021年第9期|共14页
  • 作者

    Yang Yang; Yujing Lin; Zhengchuan Zhang; Ruogu Xu; Xiaoran Yu; Feilong Deng;

  • 作者单位

    Department of Oral Implantology Guanghua School of Stomatology Hospital of Stomatology Sun Yat-Sen University Guangzhou PR China;

    Department of Oral Implantology Guanghua School of Stomatology Hospital of Stomatology Sun Yat-Sen University Guangzhou PR China;

    Department of Oral Implantology Guanghua School of Stomatology Hospital of Stomatology Sun Yat-Sen University Guangzhou PR China;

    Department of Oral Implantology Guanghua School of Stomatology Hospital of Stomatology Sun Yat-Sen University Guangzhou PR China;

    Department of Oral Implantology Guanghua School of Stomatology Hospital of Stomatology Sun Yat-Sen University Guangzhou PR China;

    Department of Oral Implantology Guanghua School of Stomatology Hospital of Stomatology Sun Yat-Sen University Guangzhou PR China;

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  • 正文语种 eng
  • 中图分类 计量学;
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