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首页> 外文期刊>Biomaterials Science >3D bioprinting dermal-like structures using species-specific ulvan
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3D bioprinting dermal-like structures using species-specific ulvan

机译：使用特定物种Ulvan的3D Bieplinting真皮样结构

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摘要

3D bioprinting has been increasingly employed in skin tissue engineering for manufacturing living constructs with three-dimensional spatial precision and controlled architecture. There is however, a bottleneck in the tunability of bioinks to address specific biocompatibility challenges, functional traits and printability. Here we report on a traditional gelatin methacryloyl (GelMA) based bioink, tuned by addition of an ulvan type polysaccharide, isolated from a cultivated source of a specific Australian Ulvacean macroalgae (Ul84). Ul84 is a sulfate- and rhamnose-rich polysaccharide, resembling mammalian glycosaminoglycans that are involved in wound healing and tissue matrix structure and function. Printable bioinks were developed by addition of methacrylated Ul84 (UlMA) to GelMA solutions. The inclusion of UlMA in the bioinks facilitated the extrusion printing process by reducing yield stress. The resultant printed structures containing ulvan exhibited improved mechanical strength and regulated the rate of scaffold degradation. The 3D printed cell-laden structures with human dermal fibroblasts demonstrated high cell viability, support of cell proliferation and dermal-like properties as evidenced by the deposition of key dermal extracellular matrix components including collagen I, collagen III, elastin and fibronectin. In vitro degradation suggested the role of UlMA in supporting structural stability of the printed cellular structures. Taken together, the present work demonstrates progression towards a biocompatible and biofunctional ink that simultaneously delivers improved mechanical, structural and stability traits that are important in facilitating real world applications in skin tissue repair.
机译：3D BioPlinting在皮肤组织工程中越来越多地用于制造具有三维空间精度和控制架构的生活构造。然而，在生物链接的可调性中，可以解决特定的生物相容性挑战，功能性状和可印刷性的瓶颈。在这里，我们报告了一种传统的明胶甲基丙烯酰（Gelma）的生物酰基，通过添加乌尔瓦型多糖调节，从特定澳大利亚Ulvacean Macroalgae（UL84）的栽培来源中分离出来。 UL84是富含硫酸盐和鼻窦的多糖，类似于哺乳动物糖胺聚糖，其参与伤口愈合和组织基质结构和功能。通过将甲基丙烯酸酯化的UL84（ULMA）（ULMA）添加到GELMA溶液，开发可印刷的生物溶液。通过降低屈服应力，在生物链中包含ulma促进了挤出印刷过程。含有ULVAN的所得印刷结构表现出改善的机械强度并调节支架降解速率。具有人的皮肤成纤维细胞的3D印刷的细胞载载体结构证明了具有通过胶原I，胶原III，弹性蛋白和纤维蛋白和纤维蛋白的关键皮肤细胞外基质组分的沉积所证明的细胞增殖和皮肤样性能的高细胞活力。体外降解表明ULMA在支持印刷细胞结构的结构稳定性方面的作用。在一起，目前的工作表明，生物相容性和生物功能墨水的进展，同时发挥了改进的机械，结构和稳定性性状，这在促进皮肤组织修复中的真实应用方面是重要的。

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  • 来源
    《Biomaterials Science》 |2021年第7期|共15页
  • 作者

    Chen Xifang; Yue Zhilian; Winberg Pia C.; Lou Yan-Ru; Beirne Stephen; Wallace Gordon G.;

  • 作者单位

    Univ Wollongong ARC Ctr Excellence Electromat Sci Intelligent Polymer Res Inst Innovat Campus Wollongong NSW 2500 Australia;

    Univ Wollongong ARC Ctr Excellence Electromat Sci Intelligent Polymer Res Inst Innovat Campus Wollongong NSW 2500 Australia;

    Venus Shell Syst Pty Ltd Huskisson NSW 2540 Australia;

    Fudan Univ Sch Pharm Dept Clin Pharm Shanghai 201203 Peoples R China;

    Univ Wollongong ARC Ctr Excellence Electromat Sci Intelligent Polymer Res Inst Innovat Campus Wollongong NSW 2500 Australia;

    Univ Wollongong ARC Ctr Excellence Electromat Sci Intelligent Polymer Res Inst Innovat Campus Wollongong NSW 2500 Australia;

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  • 正文语种 eng
  • 中图分类 分子生物学;
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