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Biomimetic nanofibrous hybrid hydrogel membranes with sustained growth factor release for guided bone regeneration

机译：具有持续生长因子释放的仿生纳米纤维杂交水凝胶膜，用于引导骨再生

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摘要

Bone regeneration, a complex physiological process, remains a challenge due to the susceptibility to the environment and absence of osteogenic growth factors around the defect region. Although autologous bone grafting is regarded as the gold standard for bone defect treatment, guided bone regeneration membranes in combination with multiple functional growth factors show a striking regeneration effect. Here, a biomimetic nanofibrous hybrid hydrogel composed of bacterial cellulose membranes and alginate/CaCl2 for sustained growth factor delivery was developed. The antibacterial peptide beta-defensin 2 served as an antibacterial, osteogenic, and angiogenic growth factor and was loaded into the aforementioned hydrogel. The mechanical and physical properties of the biomimetic nanofibrous hybrid hydrogel were investigated. Then, the in vitro osteogenic and angiogenic differentiation was confirmed by alkaline phosphatase (ALP) activity, Alizarin Red S staining, qPCR, western blot analysis and tube formation assays. After implantation into a rat calvarial defect model for 12 weeks, nanofibrous hybrid hydrogel membranes could adhere to the defect surface and promote new bone and vessel regeneration.
机译：骨再生是一种复杂的生理过程，由于对环境的易感性以及缺陷区域周围的缺乏成骨生长因子而仍然是挑战。虽然自体骨移植被视为骨缺损治疗的金标准，但是与多功能生长因子组合的引导骨再生膜显示出引人注目的再生效果。这里，开发了由细菌纤维素膜和用于持续生长因子递送的细菌纤维素膜和藻酸盐/ CaCl 2组成的仿真纳米纤维杂交水凝胶。抗菌肽β-防御蛋白2用作抗菌，骨质发生和血管生成生长因子，并装入上述水凝胶中。研究了仿生纳米纤维杂交水凝胶的机械和物理性质。然后，通过碱性磷酸酶（ALP）活性，茜素红S染色，QPCR，Western印迹分析和管形成测定来确认体外骨质发生和血管生成分化。植入大鼠颅骨缺陷模型12周后，纳米纤维杂交水凝胶膜可以粘附到缺陷表面上并促进新的骨和血管再生。

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  • 来源
    《Biomaterials Science》 |2021年第4期|共16页
  • 作者

    Yuan Qingyue; Li Lunhao; Peng Yiyu; Zhuang Ai; Wei Wei; Zhang Dandan; Pang Yan; Bi Xiaoping;

  • 作者单位

    Shanghai Jiao Tong Univ Sch Med Peoples Hosp 9 Dept Ophthalmol Shanghai Peoples R China;

    Shanghai Jiao Tong Univ Sch Med Peoples Hosp 9 Dept Ophthalmol Shanghai Peoples R China;

    Zhejiang Univ Affiliated Hosp 1 Dept Ophthalmol Zhejiang University Zhejiang Peoples R China;

    Shanghai Jiao Tong Univ Sch Med Peoples Hosp 9 Dept Ophthalmol Shanghai Peoples R China;

    Shanghai Jiao Tong Univ Sch Med Peoples Hosp 9 Dept Ophthalmol Shanghai Peoples R China;

    Shanghai Jiao Tong Univ Sch Med Peoples Hosp 9 Dept Ophthalmol Shanghai Peoples R China;

    Shanghai Jiao Tong Univ Sch Med Peoples Hosp 9 Dept Ophthalmol Shanghai Peoples R China;

    Shanghai Jiao Tong Univ Sch Med Peoples Hosp 9 Dept Ophthalmol Shanghai Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分子生物学;
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