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Synthesis and antibacterial activity of polymer-antibiotic conjugates incorporated into a resin-based dental adhesive

机译：聚合物 - 抗生素缀合物的合成和抗菌活性掺入树脂基牙科粘合剂中

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摘要

This work reports on polymer-antibiotic conjugates (PACs) as additives to resin-based restorative dental materials as a new strategy to convey sustained antibacterial character to these materials. Such antibacterial performance is expected to improve their longevity in the oral cavity. Using the previously reported ciprofloxacin (Cip)-based PAC as a control, a penicillin V (PV)-based PAC was investigated. The monomer-antibiotic conjugate (MAC) containing a methacrylate monomer group and a PV moiety was prepared via nucleophilic substitution between 2-chloroethyl methacrylate (CEMA) and penicillin V potassium (PVK). The PV-based PAC was synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization of the MAC with hydroxyethyl methacrylate (HEMA), and further characterized by H-1 NMR and gel permeation chromatography (GPC) analysis. Antibiotic resistance was investigated by passaging bacteria in low concentrations of the antibiotic for 19 days, followed by a 48 h challenge at higher concentrations. Our results suggest that the development of antibiotic resistance is unlikely. Zone of inhibition (ZOI) assays revealed no clearing zones around PV-containing resins indicating minimal antibiotic leakage from the material. Similarly, MTT assay demonstrated that the antibiotic-containing specimens did not release cytotoxic byproducts that may inhibit human gingival fibroblast growth. Counting of colony-forming units in an S. mutans biofilm model was used to assess bacterial survival at baseline and after subjecting the antibiotic-containing resin specimens to an enzymatic challenge for 30 days. Significantly reduced bacterial counts were observed as the biofilm aged from 24 to 72 h, and salivary enzymatic exposure did not reduce the antibacterial efficacy of the discs, suggesting that PV-resin will be effective in reducing the re-incidence of dental caries.
机译：该工作报告了聚合物 - 抗生素缀合物（PAC）作为树脂的修复牙科材料的添加剂，作为传送这些材料的持续抗菌性特征的新策略。预计这种抗菌性能预计将改善口腔中的寿命。使用先前报道的环丙沙星（CIP）作为对照，研究了基本霉素V（PV）PAC。通过2-氯乙基丙烯酸甲酯（CEMA）和青霉素V钾（PVK）通过亲核取代制备含有甲基丙烯酸酯单体基团和PV部分的单体 - 抗生素缀合物（MAC）。通过羟乙基甲酸乙酯（HEMA）的可逆加法 - 碎片链转移（筏）聚合，通过H-1 NMR和凝胶渗透色谱（GPC）分析，通过可逆添加 - 碎片链转移（RAFT）聚合来合成PV的PAC。通过在低浓度的抗生素中传递抗生素19天来研究抗生素抗性，然后以较高浓度攻击48小时。我们的结果表明，抗生素抗性的发展不太可能。抑制区（ZOI）测定显示出含PV的树脂周围的清除区，表明来自材料的最小抗生素泄漏。类似地，MTT测定证明含抗生素的样品未释放出可抑制人牙龈细胞生长的细胞毒性副产物。用于在S. mutans Biofilm模型中计算菌落形成单元，用于评估基线的细菌存活，并在使含抗生素的树脂标本进行酶促攻击后30天。观察到从24〜72小时的生物膜观察到显着减少的细菌计数，并且唾液酶促暴露未降低椎间盘的抗菌效果，表明PV-树脂将有效地降低龋齿的再发病率。

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  • 来源
    《Biomaterials Science》 |2021年第6期|共10页
  • 作者

    Zhang Ziwen; Jones Megan M.; Sabatini Camila; Vanyo Stephen T.; Yang Ming; Kumar Abhishek; Jiang Yancheng; Swihart Mark T.; Visser Michelle B.; Cheng Chong;

  • 作者单位

    Univ Buffalo State Univ New York Dept Chem &

    Biol Engn Buffalo NY 14260 USA;

    Univ Buffalo State Univ New York Dept Oral Biol Buffalo NY 14214 USA;

    Univ Buffalo State Univ New York Dept Restorat Dent Buffalo NY 14214 USA;

    Univ Buffalo State Univ New York Dept Oral Biol Buffalo NY 14214 USA;

    Univ Buffalo State Univ New York Dept Chem &

    Biol Engn Buffalo NY 14260 USA;

    Univ Buffalo State Univ New York Dept Chem &

    Biol Engn Buffalo NY 14260 USA;

    Univ Buffalo State Univ New York Dept Chem &

    Biol Engn Buffalo NY 14260 USA;

    Univ Buffalo State Univ New York Dept Chem &

    Biol Engn Buffalo NY 14260 USA;

    Univ Buffalo State Univ New York Dept Oral Biol Buffalo NY 14214 USA;

    Univ Buffalo State Univ New York Dept Chem &

    Biol Engn Buffalo NY 14260 USA;

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  • 正文语种 eng
  • 中图分类 分子生物学;
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