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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Cryogenically frozen PEGylated liposomes and micelles: Water penetration and polarity profiles
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Cryogenically frozen PEGylated liposomes and micelles: Water penetration and polarity profiles

机译：低温冷冻聚乙二醇化脂质体和胶束：水渗透和极性谱

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Poly(ethylene glycol) (PEG)-grafted lipid dispersions are widely investigated in fundamental and biotechnological research for their successful use in drug-delivery. Here, we consider mixtures of the bilayer-forming lipid dipalmitoylphosphatidylcholine (DPPC) with the micelle-forming lipid PEG:2000-phosphatidilethanolamine (PEG:2000-DPPE) fully hydrated in D2O and measured at 77 K. Electron Spin Echo Envelope Modulation and continuous wave Electron Paramagnetic Resonance of chain-labelled lipids are employed to detect the extent of solvent permeation and the environmental polarity, respectively, across the hydrocarbon regions of the lipid assemblies. Sigmoidal water penetration and polarity profiles are described in sterically stabilized liposomes (SSL) formed at submicellar content of PEG:2000-DPPE incorporated in DPPC. Compared to DPPC bilayers, SSL show increased hydrophobicity at both the polar/apolar interface and the chain termini, and a broader transition that is shifted toward the interface. Solvent exposure and polarity decrease on going down the chain in PEG:2000-DPPE micelles. However, compared to SSL, polymer-lipid micelles show higher solvent permeation at any chain segment and the chain termini are accessible to water. In any sample, heterogeneity is found in H-bond formation between the spin-label nitroxide groups and the solvent molecules. The results at cryogenic temperature add new insights into the biophysico-chemical characterization of PEGylated lipid dispersions.
机译：聚（乙二醇）（PEG） - 移植的脂质分散体被广泛研究，用于其成功用于药物递送的基本和生物技术研究。在此，我们认为双层成型脂质二硫代酰基磷脂酰氨基磷酰胺（DPPC）的混合物用胶束 - 形成脂质PEG：2000-磷脂硅乙醇胺（PEG：2000-DPPE）在D2O中完全水合，并在77K k.电子旋转回波包络调节和连续测量用于链标记的脂质的波浪电子顺磁共振分别在脂质组件的烃区分别检测溶剂渗透和环境极性的程度。在掺入DPPC中的PEG：2000-DPPE的亚壳含量的空间稳定的脂质体（SSL）中描述了乙状水渗透和极性曲线。与DPPC双层相比，SSL在极性/ Apolar接口和链终端中提高了疏水性，并且朝向界面移动的更宽过渡。在PEG：2000-DPPE胶束中沿着链条的溶剂暴露和极性降低。然而，与SSL相比，聚合物 - 脂质胶束在任何链段显示出较高的溶剂渗透，并可通过水偏离链末端。在任何样品中，在旋转标记硝基氧化物基团和溶剂分子之间的H键形成中发现异质性。低温温度的结果为聚乙二醇化脂质分散体的生物物质化学表征增加了新的见解。

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