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首页> 外文期刊>Biopreservation and biobanking >The Effects of Storage Time and Repeated Freeze-Thaw Cycles on Intact Fibroblast Growth Factor 23 Levels
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The Effects of Storage Time and Repeated Freeze-Thaw Cycles on Intact Fibroblast Growth Factor 23 Levels

机译：储存时间和反复冻融循环对完整成纤维细胞生长因子23水平的影响

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摘要

Background:Fibroblast growth factor 23 (FGF23) has become increasingly important in chronic kidney diseases (CKDs), cardiovascular calcification, and metabolic bone diseases. Fresh or stored blood samples are widely used for the FGF23 assay. Clarifying the factors influencing the FGF23 assay can help to quantify FGF23 more accurately. This study explored the effects of low-temperature storage time and repeated freeze-thaw cycles on the measurement of serum intact FGF23 (iFGF23). Materials and Methods:We selected 60 serum samples from patients with CKD stages 3-5 and hemodialysis patients. An enzyme-linked immunosorbent assay was used to measure the changes in serum iFGF23 levels after 6 years of storage at -80 degrees C. In total, 18 fresh serum samples were frozen and thawed for 0, 1, 3, and 5 cycles to explore the effects of repeated freeze-thaw cycles on serum iFGF23 levels. Results:Median serum iFGF23 concentrations were 252.17 (interquartile range [IQR] 113.82-592.38) pg/mL and 203.85 (IQR 64.76-545.39) pg/mL before and after 6 years. There were no significant differences between them. However, we found a downward trend of 48% in the samples close to the normal level of iFGF23 (<150.34 pg/mL) after 6 years of storage (p = 0.160). In addition, the iFGF23 levels of samples frozen and thawed for 0, 1, 3, and 5 cycles were 278.41 +/- 39.51 (mean +/- standard deviation) pg/mL, 262.84 +/- 38.42 pg/mL, 252.97 +/- 34.65 pg/mL and 250.49 +/- 37.12 pg/mL, respectively. A slight downward trend in iFGF23 levels was observed with increasing freeze-thaw times; however, no significant differences were found among different freeze-thaw cycles. Conclusion:Serum iFGF23 levels remained stable after storage at -80 degrees C for 6 years. In addition, five freeze-thaw cycles had no significant effects on serum iFGF23 levels.
机译：背景：成纤维细胞生长因子23（FGF23）在慢性肾疾病（CKDS），心血管钙化和代谢骨疾病中越来越重要。新鲜或储存的血液样品广泛用于FGF23测定。澄清影响FGF23测定的因素可以帮助更准确地量化FGF23。本研究探讨了低温储存时间和重复冻融循环对血清完整FGF23（IFGF23）测量的影响。材料和方法：我们从CKD阶段3-5患者和血液透析患者中​​选择了60个血清样本。使用酶联免疫吸附测定法测量-80℃储存6年后血清IFGF23水平的变化。总共18种新鲜血清样品被冷冻并解冻0,1,3和5次循环以探索反复冻融循环对血清IFGF23水平的影响。结果：血清IFGF23浓度中位数为252.17（间环范围[IQR] 113.82-592.38）Pg / ml和203.85（IQR 64.76-545.39）Pg / ml在6年之前和之后。它们之间没有显着差异。然而，我们发现在6年后的IFGF23（<150.34 pg / ml）的正常水平的样品中的下降趋势（p = 0.160）。此外，冷冻和解冻0,1,3和5个循环的样品的IFGF23水平为278.41 +/- 39.51（平均值+/-标准偏差）pg / ml，262.84 +/- 38.42 pg / ml，252.97 + / - 34.65 pg / ml和250.49 +/- 37.12 pg / ml。随着冻融次数增加，观察到IFGF23水平的轻微下降趋势;然而，在不同的冻融周期中没有发现显着差异。结论：血清IFGF23水平在-80摄氏度储存6年后保持稳定。此外，五个冻融循环对血清IFGF23水平没有显着影响。

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  • 来源
    《Biopreservation and biobanking》 |2021年第1期|共5页
  • 作者

    Tang Rong; Lu Yinghui; Yin Ru; Zhu Ping; Zhu Ling; Zheng Chunxia;

  • 作者单位

    Nanjing Univ Jinling Hosp Sch Med Natl Clin Res Ctr Kidney Dis 305 East Zhongshan Rd Nanjing 210002 Jiangsu Peoples R China;

    Nanjing Univ Jinling Hosp Sch Med Natl Clin Res Ctr Kidney Dis 305 East Zhongshan Rd Nanjing 210002 Jiangsu Peoples R China;

    Nanjing Univ Jinling Hosp Sch Med Natl Clin Res Ctr Kidney Dis 305 East Zhongshan Rd Nanjing 210002 Jiangsu Peoples R China;

    Nanjing Univ Jinling Hosp Sch Med Natl Clin Res Ctr Kidney Dis 305 East Zhongshan Rd Nanjing 210002 Jiangsu Peoples R China;

    Nanjing Univ Jinling Hosp Sch Med Natl Clin Res Ctr Kidney Dis 305 East Zhongshan Rd Nanjing 210002 Jiangsu Peoples R China;

    Nanjing Univ Jinling Hosp Sch Med Natl Clin Res Ctr Kidney Dis 305 East Zhongshan Rd Nanjing 210002 Jiangsu Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    iFGF23 levels; storage time; repeated freeze-thaw cycles;

    机译：IFGF23水平;储存时间;重复的冻融周期;

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