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首页> 外文期刊>Biopreservation and biobanking >Jambul (Syzygium cumini) Pulp Extract Enhances Viability, Motility, andIn VitroFertilizability of Cryopreserved Bovine Semen
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Jambul (Syzygium cumini) Pulp Extract Enhances Viability, Motility, andIn VitroFertilizability of Cryopreserved Bovine Semen

机译:Jambul(Syzygium cumini)纸浆提取物提高了冷冻保存牛精液的活力,运动,和丁基吡啶能力

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摘要

To study the ameliorative capacity ofSyzygium cuminifruit Pulp Extract (SPE) on cryopreserved bovine semen, three ejaculates each of three fertile bulls (total nine samples) were collected. Each sample was mixed with semen dilution medium in 1:2 ratios, centrifuged, and 50% of the supernatant was discarded. The remaining sample was mixed with semen cryopreservation extender (SCE) in 1:4 ratios. Three aliquots (0.3 mL) of each extended semen sample were further diluted with (1) 0.7 mL SCE (SCE group), (2) 0.7 mL SCE containing SPE equivalent to 7 ppm total antioxidants (SPE7 group), and (3) 0.7 mL SCE equivalent to 14 ppm antioxidants (SPE14 group). For each aliquot, semen quality was analyzedin situas well as after cryopreservation. Results show significant improvement in sperm membrane integrity, motility, and fertilizability in SPE7 (63.5% +/- 2.04%, 56.22% +/- 1.7%, 51.1%, respectively) and SPE14 (68% +/- 1.12%, 63.67% +/- 1.06%, 57.7%, respectively) than the SCE group (62.33% +/- 1.22%, 54.9% +/- 1.34%, 48.8%, respectively). Similarly, significant improvement in the mean percent number of progressively motile sperm at the expense of the mean percent twitching, shaking, and whirling spermatozoa, both before and after cryopreservation, were observed in SPE7 (72 +/- 1.06, 61.2 +/- 1.2) and SPE14 (78.89 +/- 1.12, 67 +/- 1.32) groups to that of the SCE (59.6 +/- 0.97, 52.3 +/- 3.5). In addition, the mean percent of progressively motile spermatozoa showing 20 mu m/s or above velocity, before and after cryopreservation, were significantly higher in SPE7 (58.9 +/- 4.7, 43.6 +/- 1.5) and SPE14 (66.9 +/- 0.8, 51.3 +/- 0.8) groups than that of the SCE (42.3 +/- 3.1, 30 +/- 2.4). These findings indicate that SPE can significantly improve various quality parameters of the cryopreserved bovine semen.
机译:为研究昔氨宫纸浆提取物(SPE)对冷冻保存的牛精液的改善能力,收集了三种肥沃的公牛(总九个样品)中的三种射精。将每种样品与精液稀释培养基混合在1:2的比例中,离心,并弃去50%的上​​清液。将剩余的样品与精液冷冻保存增量剂(SCE)混合在1:4比率。将每个延长的精液样品中的三种等分试样(0.3mL)进一步用(1)0.7mL SCE(SCE基团),(2)0.7mL Sce含有相当于7ppm总抗氧化剂(SPE7组),(3)0.7 ML SCE相当于14ppm抗氧化剂(SPE14组)。对于每次等分试样,精液质量在低温保存之后分析鉴定鉴定。结果表现出SPE7中精子膜完整性,运动和肥力的显着改善(63.5%+/- 2.04%,51.1%,分别为51.1%)和SPE14(68%+/- 1.12%,63.67% +/- 1.06%,分别比SCE集团(62.33%+/- 1.22%,54.9%+/- 1.34%,分别为48.8%)。类似地,在SPE7(72 +/- 1.06,61.2 +/- 1.2之前,在冷冻保存之前和之后的平均百分比的平均抽搐,摇动和旋转精子的平均动机精子的平均百分比的平均百分比的显着改善(72 +/- 1.06,61.2 +/- 1.2 )和SPE14(78.89 +/- 1.12,67 +/- 1.32)组到SCE的组(59.6 +/- 0.97,52.3 +/- 3.5)。此外,SPE7之前和后,在冷冻保存之前和之后的逐步动机精子的平均百分比均为20μm/ s或高于速度,显着高出显着高(58.9 +/- 4.7,43.6 +/- 1.5)和SPE14(66.9 +/- 0.8,51.3 +/- 0.8)组比SCE的组(42.3 +/- 3.1,30 +/- 2.4)。这些发现表明,SPE可以显着改善冷冻保存的牛精液的各种质量参数。

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