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Inline noninvasive Raman monitoring and feedback control of glucose concentration during ethanol fermentation

机译：乙醇发酵过程中葡萄糖浓度的内联无侵入式拉曼监测和反馈控制

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Abstract > Raman spectroscopy as a process analytical technology tool was implemented for the monitoring and control of ethanol fermentation carried out with Saccharomyces cerevisiae . The need for the optimization of bioprocesses such as ethanol production, to increase product yield, enhanced the development of control strategies. The control system developed by the authors utilized noninvasive Raman measurements to avoid possible sterilization problems. Real‐time data analysis was applied using partial least squares regression (PLS) method. With the aid of spectral pretreatment and multivariate data analysis, the monitoring of glucose and ethanol concentration was successful during yeast fermentation with the prediction error of 4.42?g/L for glucose and 2.40?g/L for ethanol. By Raman spectroscopy‐based feedback control, the glucose concentration was maintained at 100?g/L by the automatic feeding of concentrated glucose solution. The control of glucose concentration during fed‐batch fermentation resulted in increased ethanol production. Ethanol yield of 86% was achieved compared to the batch fermentation when cssStyle="text-decoration:underline">75 % yield was obtained. The results show that the use of Raman spectroscopy for the monitoring and control of yeast fermentation is a promising way to enhance process understanding and achieve consistently high production yield. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 作为过程分析技术工具的拉曼光谱学用于监测和控制与之进行的乙醇发酵 Saccharomyces cerevisiae </ i> 。需要优化乙醇生产等生物过程，以提高产品产量，增强控制策略的发展。作者开发的控制系统利用非侵入式拉曼测量来避免可能的灭菌问题。使用偏最小二乘回归（PLS）方法应用实时数据分析。借助光谱预处理和多变量数据分析，在酵母发酵期间葡萄糖和乙醇浓度的监测成功，其预测误差为4.42Ω·克隆的葡萄糖和2.40μl乙醇。通过拉曼光谱基反馈控制，通过自动进料浓缩葡萄糖溶液，在100μlg/ l处保持葡萄糖浓度。对氟化批发酵过程中葡萄糖浓度的控制导致乙醇产量增加。与批量发酵相比，乙醇产率为86％ cssstyle =“文本装饰：下划线”> 75 </ span> 获得％产率。结果表明，使用拉曼光谱对酵母发酵的监测和控制是一种有希望的方法，可以增强过程理解，实现一致的高产量。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15005/'>《Biotechnology Progress》</a> <b style="margin: 0 2px;">|</b><span>2019年第5期</span><b style="margin: 0 2px;">|</b><span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hirsch Edit&option=202" target="_blank" rel="nofollow">Hirsch Edit;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pataki Hajnalka&option=202" target="_blank" rel="nofollow">Pataki Hajnalka;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Domján Júlia&option=202" target="_blank" rel="nofollow">Domján Júlia;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Farkas Attila&option=202" target="_blank" rel="nofollow">Farkas Attila;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Vass Panna&option=202" target="_blank" rel="nofollow">Vass Panna;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fehér Csaba&option=202" target="_blank" rel="nofollow">Fehér Csaba;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Barta Zsolt&option=202" target="_blank" rel="nofollow">Barta Zsolt;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nagy Zsombor K.&option=202" target="_blank" rel="nofollow">Nagy Zsombor K.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Marosi Gy?rgy J.&option=202" target="_blank" rel="nofollow">Marosi Gy?rgy J.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Csontos István&option=202" target="_blank" rel="nofollow">Csontos István;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Organic Chemistry and TechnologyBudapest University of Technology and EconomicsBudapest Hungary;</p> <p>Department of Organic Chemistry and TechnologyBudapest University of Technology and EconomicsBudapest Hungary;</p> <p>Department of Organic Chemistry and TechnologyBudapest University of Technology and EconomicsBudapest Hungary;</p> <p>Department of Organic Chemistry and TechnologyBudapest University of Technology and EconomicsBudapest Hungary;</p> <p>Department of Organic Chemistry and TechnologyBudapest University of Technology and EconomicsBudapest Hungary;</p> <p>Department of Applied Biotechnology and Food ScienceBudapest University of Technology and EconomicsBudapest Hungary;</p> <p>Department of Applied Biotechnology and Food ScienceBudapest University of Technology and EconomicsBudapest Hungary;</p> <p>Department of Organic Chemistry and TechnologyBudapest University of Technology and EconomicsBudapest Hungary;</p> <p>Department of Organic Chemistry and TechnologyBudapest University of Technology and EconomicsBudapest Hungary;</p> <p>Department of Organic Chemistry and TechnologyBudapest University of Technology and EconomicsBudapest Hungary;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/15.html" title="生物科学">生物科学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=bioprocess control&option=203" rel="nofollow">bioprocess control;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=closed‐loop feedback control&option=203" rel="nofollow">closed‐loop feedback control;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=ethanol fermentation&option=203" rel="nofollow">ethanol fermentation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=industry 4.0&option=203" rel="nofollow">industry 4.0;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=inline noninvasive Raman spectroscopy&option=203" rel="nofollow">inline noninvasive Raman spectroscopy;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=PAT&option=203" rel="nofollow">PAT;</a> </p> <div class="translation"> 机译：生物过程控制;闭环反馈控制;乙醇发酵;工业4.0;内联无侵入拉曼光谱;拍; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-journal-chemical-engineering_thesis/0201245369408.html">The Effect of pH Control on Acetone-Butanol-Ethanol Fermentation by Clostridium acetobutylicum ATCC 824 with Xylose and D-Glucose and D-Xylose Mixture</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wei Jiang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Wei Jiang,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhiqiang Wen&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zhiqiang Wen,</a> <a 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href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Braun Frank&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Braun Frank,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Frank Rudolf&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Frank Rudolf,</a> <a href="/journal-foreign-915/" target="_blank" rel="nofollow" class="tuijian_authcolor">Bioprocess and Biosystems Engineering .</a> <span>2017</span><span>,第10期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：非接触式拉曼光谱仪可在酵母发酵过程中在线监测葡萄糖和乙醇</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704020156604.html">Monitoring alcohol concentration and residual glucose in solid state fermentation of ethanol using FT-NIR spectroscopy and L1-PLS regression</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hui Jiang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Hui Jiang,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Congli Mei&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Congli Mei,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kangji Li&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Kangji Li,</a> <a href="/journal-foreign-28590/" target="_blank" rel="nofollow" class="tuijian_authcolor">Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy .</a> <span>2018</span><span>,第期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：使用FT-NIR光谱和L1-PLS回归监测乙醇固态发酵中的醇浓度和残留葡萄糖</span> </p> </li> <li> <div> <b>8. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_meeting-226286_thesis/020516874542.html">Terahertz quantification of ethanol and sugar concentrations in water and its application for noninvasive real-time monitoring of fermentation</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Olutosin Fawole&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Olutosin Fawole,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Massood Tabib-Azar&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Massood Tabib-Azar </a> <a href="/conference-foreign-226286/" target="_blank" rel="nofollow" class="tuijian_authcolor">IEEE MTT-S International Microwave Symposium .</a> <span>2016</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：太赫兹定量分析水中乙醇和糖的浓度及其在发酵无创实时监测中的应用</span> </p> </li> <li> <div> <b>9. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/02061362092.html">Measurement of tissue optical properties with optical coherence tomography: Implication for noninvasive blood glucose concentration monitoring.</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Larin, Kirill V.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Larin, Kirill V. </a> <span>2002</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用光学相干断层扫描技术测量组织的光学特性：对无创血糖浓度监测的意义。</span> </p> </li> <li> <div> <b>10. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040003959079.html">A predictive and feedback control algorithm maintains a constant glucose concentration in fed-batch fermentations.</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=G L Kleman&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">G L Kleman,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=J J Chalmers&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">J J Chalmers,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=G W Luli&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">G W Luli,</a> <span>1991</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：预测和反馈控制算法可在分批补料发酵中保持恒定的葡萄糖浓度。</span> </p> </li> <li> <div> <b>11. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/01000126372703.html">Inline noninvasive Raman monitoring and feedback control of glucose concentration during ethanol fermentation</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Edit Hirsch&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Edit Hirsch,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hajnalka Pataki&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Hajnalka Pataki,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Júlia Domján&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Júlia Domján,</a> <span>2019</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：在乙醇发酵期间内联无血管拉曼监测和反馈控制葡萄糖浓度</span> </p> </li> <li> <div> <b>12. </b><a class="enjiyixqcontent" href="/ntis-science-report_ad_thesis/02071492292.html">Surface Enhanced Raman Spectroscopy for Monitoring Lactate and Glucose</a> <b>[R] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Glucksberg, M.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Glucksberg, M.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Walsh, J.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Walsh, J.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Duyne, R. V.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Duyne, R. V. </a> <span>2006</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用于监测乳酸和葡萄糖的表面增强拉曼光谱</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-journal-chemical-engineering-english_thesis/0201297373161.html">Continuous Ethanol Production Through Fermentation with Glucose and Xylose by Immobilized Yeasts in Two Cascade Fixed Beds</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=邓旭&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 邓旭</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=岑沛霖&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,岑沛霖</a> <span> <a href="/journal-cn-52877/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国化学工程学报：英文版 </a> </span> <span> . 1998</span><span>,第1期</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06130500431635.html">2 NONINVASIVE-TYPE MONITORING SENSOR SYSTEM FOR MEASURING BLOOD GLUCOSE USING TWO WAVELENGTH RAMAN SCATTERING</a> <b>[P]</b> . <span> 外国专利：  KR20210092969A </span> <span> . 2021-07-27</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：2使用两个波长拉曼散射测量血糖的非型式监测传感器系统 </span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06130500887071.html">NONINVASIVE-TYPE MONITORING SENSOR SYSTEM FOR MEASURING BLOOD GLUCOSE USING TWO WAVELENGTH RAMAN SCATTERING</a> <b>[P]</b> . <span> 外国专利：  KR102315843B1 </span> <span> . 2021-10-22</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：使用两个波长拉曼散射测量血糖的非侵入式监测传感器系统 </span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130456036333.html">Recombinant yeast effective vector recombinant DNA molecule for yeast transformation process to obtain an effective recombinant yeast for fermentation of xylose to ethanol process for fermentation of glucose to ethanol and recombinant yeast</a> <b>[P]</b> . <span> 外国专利：  BR9408010A </span> <span> . 1996-12-17</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用于酵母转化过程的重组酵母有效载体重组DNA分子，获得用于木糖发酵成乙醇的有效重组酵母，用于葡萄糖发酵成乙醇的重组酵母和重组酵母 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div 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