• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Biotransformation of -hydroxypyruvate and glycolaldehyde to l-erythrulose by Pichia pastoris strain GS115 overexpressing native transketolase
【24h】

Biotransformation of -hydroxypyruvate and glycolaldehyde to l-erythrulose by Pichia pastoris strain GS115 overexpressing native transketolase

机译：用Pichia牧场菌菌株GS115过表达Transpterol酶的磷吡替金酸酯和甘醇醛与L-erythrulose的生物转化

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Transketolase is a proven biocatalytic tool for asymmetric carbon-carbon bond formation, both as a purified enzyme and within bacterial whole-cell biocatalysts. The performance of Pichia pastoris as a host for transketolase whole-cell biocatalysis was investigated using a transketolase-overexpressing strain to catalyze formation of l-erythrulose from -hydroxypyruvic acid and glycolaldehyde substrates. Pichia pastoris transketolase coding sequence from the locus PAS_chr1-4_0150 was subcloned downstream of the methanol-inducible AOX1 promoter in a plasmid for transformation of strain GS115, generating strain TK150. Whole and disrupted TK150 cells from shake flasks achieved 62% and 65% conversion, respectively, under optimal pH and methanol induction conditions. In a 300 mu L reaction, TK150 samples from a 1L fed-batch fermentation achieved a maximum l-erythrulose space time yield (STY) of 46.58 g L-1 h(-1), specific activity of 155 U g(CDW)(-1), product yield on substrate (Y-p/s) of 0.52 mol mol(-1) and product yield on catalyst (Y-p/x) of 2.23g g(CDW)(-1). We have successfully exploited the rapid growth and high biomass characteristics of Pichia pastoris in whole cell biocatalysis. At high cell density, the engineered TK150 Pichia pastoris strain tolerated high concentrations of substrate and product to achieve high STY of the chiral sugar L-erythrulose. (c) 2017 The Authors Biotechnology Progress published by Wiley Periodicals, Inc. on behalf of American Institute of Chemical Engineers Biotechnol. Prog., 34:99-106, 2018
机译：Transportolase是一种经过验证的生物催化工具，用于不对称碳 - 碳键形成，无论是纯化的酶还是细菌全细胞生物催化剂。利用转铁糖蛋白过表达应变研究了毕赤酵母作为转铁糖酶全细胞生物催化的宿主的性能，以催化来自 - 羟基吡咯酸和甘醇醛底物的L-erythrulose的形成。从基因岛PAS_CHR1-4_4_4_4_0150中的Pichia Pastoris Transportolase编码序列在甲醇诱导的AxOx1启动子的下游亚克隆到质粒上，用于转化菌株GS115，产生菌株TK150。在最佳pH和甲醇诱导条件下，来自摇瓶的全部和破坏的TK150细胞分别达到62％和65％的转化率。在300μl反应中，TK150来自1L FED批量发酵的样品实现了最大L-季节糖空间产率（STY）为46.58g L-1 H（-1），155ug（CDW）的比活性（CDW）（ -1），在2.23gg（CdW）（CdW）（ - 1）的催化剂（Yp / x）上的产物产率为0.52mol mol（-1）和产物产率。我们成功地利用了全细胞生物分析的Pichia Pastoris的快速增长和高生物量特征。在高电池密度下，工程化TK150磷蛋白抗牧场菌菌株耐受高浓度的基材和产物，以实现手性糖L-erykrulose的高浓度。 （c）2017年作者Biotechnology通过Wiley Hearyichs，Inc。代表美国化学工程师Biotechnol。 Prog。，34：99-106，2018

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号