首页> 外文期刊>Biotechnology Progress >Lectin bio‐layer interferometry for assessing product quality of Fc‐ glycosylated immunoglobulin G

【24h】

Lectin bio‐layer interferometry for assessing product quality of Fc‐ glycosylated immunoglobulin G

机译：凝集素生物层干涉测量评估FC-糖基化免疫球蛋白G的产品质量

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Abstract > Glycosylation, as the most prominent posttranslational modification, is recognized as an important quality attribute of monoclonal antibodies affected by various bioprocess parameters and cellular physiology. A method of lectin‐based bio‐layer interferometry (LBLI) to relatively rank galactosylation and fucosylation levels was developed. For this purpose, Fc‐glycosylated immunoglobulin G (IgG) was recombinantly produced with varying bioprocess conditions in 15?L bioreactor and accumulated IgG was harvested. The reliability, the robustness and the applicability of LBLI to different samples has been proven. Data obtained from LC–MS analysis served as reference and were compared to the LBLI results. The introduced method is based on non‐fluidic bio‐layer interferometry (BLI), which becomes recently a standard tool for determining biomolecular interactions in a label‐free, real‐time and high‐throughput manner. For the intended purpose, biotinylated lectins were immobilized on disposable optical fiber streptavidin (SA) biosensor tips. Aleuria aurantia lectin (AAL) was used to detect the core fucose and <fi>Ricinus communis</fi> agglutinin 120 (RCA120) to determine galactosylation levels. In our case study it could be shown that fucosylation was not affected by variations in glucose feed concentration and cultivation temperature. However, the galactosylation could be correlated with the ratio of mean specific productivity (q <sub>P</sub> ) and ammonium (q <sub>NH4+</sub> ) but was unrelated to the ratio of mean q <sub>P</sub> and the specific glucose consumption (q <sub>gluc</sub> ). This presented method strengthens the applicability of the BLI platform, which already enables measurement of several product related characteristics, such as product quantity as well as kinetic rates (k <sub>d</sub> ,k <sub>on</sub> ) and affinity constants (k <sub>D</sub> ) analysis. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 作为最突出的后翻译改性糖基化被认为是受各种生物过程和细胞生理学影响的单克隆抗体的重要品质属性。开发了一种基于凝集素的生物层干涉法（LBLI）的方法，以相对级含半乳糖基化和岩藻糖基化水平。为此目的，通过在15μlbioRealctor中的不同生物过程条件下重组产生Fc-糖基化的免疫球蛋白G（IgG），并收获积累的IgG。已经证明了LBLI对不同样品的可靠性，鲁棒性和适用性。从LC-MS分析获得的数据用作参考，并与LBLI结果进行比较。介绍的方法基于非流体生物层干涉测量法（BLI），其最近成为用于在无标记，实时和高吞吐量的基础上确定生物分子相互作用的标准工具。对于预期目的，将生物素化凝集素固定在一次性光纤链霉抗生物素蛋白（SA）生物传感器尖端上。 Aleuria宫炎</ i> 凝集素（aal）用于检测核心岩藻糖和 <FI> Ricinus Communis </ Fi> AgGlutinin 120（RCA120）确定半乳糖基化水平。在我们的案例中，可以表明岩藻糖基化不受葡萄糖饲料浓度和培养温度的变化的影响。然而，半乳糖基化可以与平均特定生产率的比率相关（Q <sub> p </ sub> ）和铵（q <sub> nh4 + </ sub> ）但与平均Q的比率无关 <sub> p </ sub> 和特定的葡萄糖消费（q <sub> gluc </ sub> ）。该提出的方法加强了BLI平台的适用性，该平台已经能够测量几种产品相关特征，例如产品量以及动力速率（K. <sub> d </ sub> ，K <sub>在</ sub>上）和亲和力常数（k <sub> d </ sub> ）分析。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15005/'>《Biotechnology Progress》</a> <b style="margin: 0 2px;">|</b><span>2019年第5期</span><b style="margin: 0 2px;">|</b><span>共9页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wallner Jakob&option=202" target="_blank" rel="nofollow">Wallner Jakob;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sissolak Bernhard&option=202" target="_blank" rel="nofollow">Sissolak Bernhard;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sommeregger Wolfgang&option=202" target="_blank" rel="nofollow">Sommeregger Wolfgang;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lingg Nico&option=202" target="_blank" rel="nofollow">Lingg Nico;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Striedner Gerald&option=202" target="_blank" rel="nofollow">Striedner Gerald;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Vorauer‐Uhl Karola&option=202" target="_blank" rel="nofollow">Vorauer‐Uhl Karola;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of BiotechnologyUniversity of Natural Resources and Life SciencesVienna Austria;</p> <p>Research and DevelopmentBilfinger Industrietechnik Salzburg GmbHSalzburg Austria;</p> <p>Research and DevelopmentBilfinger Industrietechnik Salzburg GmbHSalzburg Austria;</p> <p>Department of BiotechnologyUniversity of Natural Resources and Life SciencesVienna Austria;</p> <p>Department of BiotechnologyUniversity of Natural Resources and Life SciencesVienna Austria;</p> <p>Department of BiotechnologyUniversity of Natural Resources and Life SciencesVienna Austria;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/15.html" title="生物科学">生物科学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=bio‐layer interferometry&option=203" rel="nofollow">bio‐layer interferometry;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=CHO cell culture&option=203" rel="nofollow">CHO cell culture;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=fucosylation&option=203" rel="nofollow">fucosylation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=galactosylation&option=203" rel="nofollow">galactosylation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=glycosylation&option=203" rel="nofollow">glycosylation;</a> </p> <div class="translation"> 机译：生物层干涉测量;Cho细胞培养;岩藻糖基化;半乳糖基化;糖基化; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-systems-science-information_thesis/020121903076.html">Using Multi-input-layer Wavelet Neural Network to Model Product Quality of Continuous Casting Furnace and Hot Rolling Mill</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=HuanqinLi&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">HuanqinLi,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=JieCheng&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">JieCheng,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=BaiwuWan&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">BaiwuWan </a> <a href="/journal-foreign-19116/" target="_blank" rel="nofollow" class="tuijian_authcolor">系统科学与信息学报：英文版 .</a> <span>2004</span><span>,第002期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704054366.html">Interaction of the low-affinity receptor CD23/Fc epsilonRII lectin domain with the Fc epsilon3-4 fragment of human immunoglobulin E.</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Shi J&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Shi J,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ghirlando R&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Ghirlando R,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Beavil RL&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Beavil RL,</a> <a href="/journal-foreign-14809/" target="_blank" rel="nofollow" class="tuijian_authcolor">Biochemistry .</a> <span>1997</span><span>,第8期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：低亲和力受体CD23 /FcεRII凝集素域与人免疫球蛋白EFcε3-4片段的相互作用。</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-foreign_detail_thesis/0204117422102.html">Assessing <fc>HIV</fc> and <fc>AIDS</fc> treatment safety and health‐related quality of life among cohort of <fc>M</fc>alaysian patients: a discussion on methodological approach</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Imran Ahmed Syed&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Imran Ahmed Syed,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Syed Azhar Syed Sulaiman&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Syed Azhar Syed Sulaiman,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mohammad Azmi Hassali&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Mohammad Azmi Hassali,</a> <a href="/journal-foreign-32381/" target="_blank" rel="nofollow" class="tuijian_authcolor">Health expectations: an international journal of public participation in health care and health policy .</a> <span>2015</span><span>,第5期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：评估<fc> M </ fc>阿拉亚患者队列中的<fc> HIV </ fc>和<fc> AIDS </ fc>治疗安全性及与健康相关的生活质量：方法论讨论</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-foreign_detail_thesis/0204118913162.html">Mutational deglycosylation of the Fc portion of immunoglobulin G causes O‐sulfation of tyrosine adjacently preceding the originally glycosylated site</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> </a> <a href="/journal-foreign-26238/" target="_blank" rel="nofollow" class="tuijian_authcolor">FEBS Letters .</a> <span>2010</span><span>,第15期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：免疫球蛋白G Fc部分的突变去糖基化会导致酪氨酸的O-硫酸化在原糖基化位点之前</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_meeting-227392_thesis/020516745227.html">Assessing the quality of Global Human Settlement Layer products for Kampala, Uganda</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Richard Sliuzas&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Richard Sliuzas,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Monika Kuffer&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Monika Kuffer,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Thomas Kemper&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Thomas Kemper </a> <a href="/conference-foreign-227392/" target="_blank" rel="nofollow" class="tuijian_authcolor">Joint Urban Remote Sensing Event .</a> <span>2017</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：评估乌干达坎帕拉的全球人类住区层产品的质量</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/0206130533.html">Assessing quality planning development of system-to-system ISO 7 application-layer system interface definitions.</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Czerwinski, John J.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Czerwinski, John J. </a> <span>2010</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：评估系统到系统ISO 7应用层系统接口定义的质量计划开发。</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040006201448.html">Bio-Layer Interferometry Analysis of the Target Binding Activity of CRISPR-Cas Effector Complexes</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hanna Müller-Esparza&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Hanna Müller-Esparza,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Manuel Osorio-Valeriano&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Manuel Osorio-Valeriano,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Niklas Steube&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Niklas Steube,</a> <span>2020</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：CRISPR-Cas效应复合物靶结合活性的生物层干涉分析</span> </p> </li> <li> <div> <b>8. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/01000124273107.html">Lectin bio‐layer interferometry for assessing product quality of Fc‐ glycosylated immunoglobulin G</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jakob Wallner&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Jakob Wallner,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bernhard Sissolak&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Bernhard Sissolak,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wolfgang Sommeregger&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Wolfgang Sommeregger,</a> <span>2019</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用于评估Fc-糖基化免疫球蛋白G的产品质量的凝集生物层干涉测量</span> </p> </li> <li> <div> <b>9. </b><a class="enjiyixqcontent" href="/ntis-science-report_de_thesis/02071215506.html">Assessing the Economic Viability of Bio-based Products for Missouri Value-added Crop Production. Report Period August 1, 2001-July 31, 2004</a> <b>[R] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> </a> <span>2005</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：评估密苏里州增值作物生产中生物基产品的经济可行性。报告期限2001年8月1日至2004年7月31日</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06130425840092.html">Precipitating Substrate for Bio-Layer Interferometry</a> <b>[P]</b> . <span> 外国专利：  US2011236911A1 </span> <span> . 2011-09-29</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用于生物层干涉测量的沉淀基质 </span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06130429753482.html">ENZYME ACTIVITY MEASUREMENTS USING BIO-LAYER INTERFEROMETRY</a> <b>[P]</b> . <span> 外国专利：  EP1833980A4 </span> <span> . 2009-02-18</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：使用生物层干涉法测量酶活性 </span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130430847070.html">Enzyme Activity Measurements Using Bio-Layer Interferometry</a> <b>[P]</b> . <span> 外国专利：  US2009068694A1 </span> <span> . 2009-03-12</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：使用生物层干涉法测量酶活性 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704028287699','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div style="display: flex;margin-top: 69px;justify-content: space-between;"> <div class="no-sub" onclick="$('.journalsub-pop-up').hide()">暂不订阅</div> <div class="other-sub" onclick="continueSub('from=pc-detail')">继续订阅其他期刊</div> </div> </div> </div> <div class="right_btn"> <ul> <li class="gouwuche">  </li> <li class="yijian"> <a href="javascript:void(0);" onclick="link_analysis('/mycenter/auth/complaint.html',this)">意见反馈</a> </li> <li class="top"> <a href="javascript:scrollTo(0,0);">回到顶部</a> </li> <li class="shouye"> <a href="/">回到首页</a> </li> </ul> </div> <div class="xllindexfooter"> <div class="xllindexfootercenter"> <div class="xllindexfooterleft left" > <div class="xllindexfooterleftli"> <ul> <li><a href="/about.html">关于掌桥</a></li> <li><a href="/help/helpmap.html">资源导航</a></li> <li><a href="/help/helpguide.html">新手指南</a></li> <li><a href="/help/helpcenter.html">常见问题</a></li> <li><a href="/sitemap.html">网站地图</a></li> <li><a href="/help/helpcenter.html?type=9">版权声明</a></li> </ul> </div> <div class="xllindexfooterleft"> <p class="xllindexfooterlefteamil">客服邮箱：kefu@zhangqiaokeyan.com</p> <div class="xllindexfooterlefttcp"> <div class="xllindexfooterpoliceiimg"></div> <div class="xllindexfooterpoliceispan"> <span>京公网安备：11010802029741号 </span> <span>ICP备案号：<a href="https://beian.miit.gov.cn" rel="nofollow" target="_blank">京ICP备15016152号-6</a></span> <span>六维联合信息科技 (北京) 有限公司©版权所有</span> </div> </div> </div> </div> <div class="xllindexfooterright left"> <ul> <li> <p style="font-weight: bold;">客服微信</p> <div></div> </li> <li> <p style="font-weight: bold;">服务号</p> <div></div> </li> </ul> </div> </div> </div> <span id="0704028287699down" data-source="7," data-out-id="ks0fRsyRetuQt1BCw4aq66WWnLmM25trSXWHPekIdVSqzu8+VO0U2GRbLE0869vM," data-f-source-id="7" data-title="Lectin bio‐layer interferometry for assessing product quality of Fc‐ glycosylated immunoglobulin G" data-price="20" data-site-name="" data-transnum="24" style="display:none;"></span> <input type="hidden" value="4" id="sourcetype"> <input type="hidden" value="15005" id="journalid"> <input type="hidden" value="1" id="inyn_provide_service_level"> <input type="hidden" value="https://cdn.zhangqiaokeyan.com" id="imgcdn"> <input type="hidden" value="1" id="isdeatail"> <input type="hidden" value="" id="syyn_indexed_database"> <input type="hidden" value="" id="servicetype"> <input type="hidden" id="pagename" value="Lectin bio‐layer interferometry for assessing product quality of Fc‐ glycosylated immunoglobulin G"/> <input type="hidden" value="thesis_get_original" id="pageIdentification"> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery-1.12.4.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/lwlh_ajax.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zq.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/common.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery.cookie.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/top.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/tip.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/login.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/down.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/search.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/searchtype.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/user/regist.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/detail.js?v=5.7.7"></script> <script type="text/javascript" src="https://www.zhangqiaokeyan.com/statistics/static/pagecollection.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/tj.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/pushbaidu.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/history.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/util/cookie.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/weipu/weipu.js?v=5.7.7"></script> </body> <script> $(function(){ var weiPuStatus = getCookie('WeiPuStatus'); if(weiPuStatus){ tipWeiPuStatus(weiPuStatus); delCookie('WeiPuStatus'); } getFacetKeywordVoInId(); var sourcetype = $("#sourcetype").val(); var inyn_provide_service_level = $("#inyn_provide_service_level").val(); if((sourcetype ==1||sourcetype==4)&&inyn_provide_service_level!=4){ getJournal(); } }) </script> </html>