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首页> 外文期刊>Biotechnology Progress >Comprehensive studies on optimization of cellulase and xylanase production by a local indigenous fungus strain via solid state fermentation using oil palm frond as substrate
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Comprehensive studies on optimization of cellulase and xylanase production by a local indigenous fungus strain via solid state fermentation using oil palm frond as substrate

机译：用油棕榈叶作为底物通过固态发酵通过固态发酵优化纤维素酶和木聚糖酶产生的综合研究

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Abstract > The high cost of cellulases remains the most significant barrier to the economical production of bio‐ethanol from lignocellulosic biomass. The goal of this study was to optimize cellulases and xylanase production by a local indigenous fungus strain ( Aspergillus niger DWA8) using agricultural waste (oil palm frond [OPF]) as substrate. The enzyme production profile before optimization indicated that the highest carboxymethyl cellulose (CMCase), filter paper (FPase), and xylanase activities of 1.06?U/g, 2.55?U/g, and 2.93?U/g were obtained on day 5, day 4, and day 5 of fermentation, respectively. Response surface methodology was used to study the effects of several key process parameters in order to optimize cellulase production. Of the five physical and two chemical factors tested, only moisture content of 75% (w/w) and substrate amount of 2.5 g had statistically significant effect on enzymes production. Under optimized conditions of 2.5 g of substrate, 75% (w/w) moisture content, initial medium of pH 4.5, 1?×?10 6 spores/mL of inoculum, and incubation at ambient temperature (±30°C) without additional carbon and nitrogen, the highest CMCase, FPase, and xylanase activities obtained were 2.38?U/g, 2.47?U/g, and 5.23?U/g, respectively. Thus, the optimization process increased CMCase and xylanase production by 124.5 and 78.5%, respectively. Moreover, <fc> <fi>A. niger</fi> </fc> DWA8 produced reasonably good cellulase and xylanase titers using OPF as the substrate when compared with previous researcher finding. The enzymes produced by this process could be further use to hydrolyze biomass to generate reducing sugars, which are the feedstock for bioethanol production. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 纤维素酶的高成本仍然是从木质纤维素生物质的生物乙醇经济生产的最显着障碍。本研究的目标是通过局部土着真菌菌株进行纤维素酶和木聚糖酶生产（ 曲霉尼日尔</ i> DWA8）使用农业废物（油棕熔液[OPF]）作为基材。优化前的酶生产曲线表明，最高的羧甲基纤维素（CMCase），滤纸（FPase）和木聚糖酶活性为1.06？U / G，2.55？U / G和2.93？U / g在第5天获得，第4天和第5天发酵。响应表面方法用于研究几个关键过程参数的影响，以优化纤维素酶生产。在测试的五种物理和两种化学因素中，只有75％（w / w）的水分含量和2.5g的底物量对酶产生具有统计学显着的影响。在优化的条件下为2.5g底物，75％（w / w）水分含量，pH 4.5，1的初始介质，1？×10 6 </ sup> 在没有额外的碳和氮气的环境温度（±30℃）的环境温度（±30℃），最高的Cmcase，FPase和含有木聚糖酶活性的环境温度（±30℃）的孵育为2.38？U / g，2.47？U / g和5.23？U. / g分别。因此，优化过程分别将Cmcase和木聚糖酶产量增加124.5％和78.5％。而且， <FC> <fi> a。尼日尔</ fi> </ FC> 与先前的研究人员发现相比，DWA8使用OPF产生合理的纤维素酶和木聚糖酶滴度。通过该方法产生的酶可以进一步用于水解生物质以产生还原糖，这是生物乙醇生产的原料。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15005/'>《Biotechnology Progress》</a> <b style="margin: 0 2px;">|</b><span>2019年第3期</span><b style="margin: 0 2px;">|</b><span>共14页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tai Wan Yi&option=202" target="_blank" rel="nofollow">Tai Wan Yi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tan Joo Shun&option=202" target="_blank" rel="nofollow">Tan Joo Shun;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lim Vuanghao&option=202" target="_blank" rel="nofollow">Lim Vuanghao;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lee Chee Keong&option=202" target="_blank" rel="nofollow">Lee Chee Keong;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Bioprocess Technology Division School of Industrial TechnologyUniversiti Sains MalaysiaPenang Malaysia;</p> <p>Bioprocess Technology Division School of Industrial TechnologyUniversiti Sains MalaysiaPenang Malaysia;</p> <p>Integrative Medicine ClusterAdvanced Medical and Dental Institute Universiti Sains MalaysiaPenang Malaysia;</p> <p>Bioprocess Technology Division School of Industrial TechnologyUniversiti Sains MalaysiaPenang Malaysia;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/15.html" title="生物科学">生物科学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Aspergillus niger&option=203" rel="nofollow">Aspergillus niger;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=cellulases&option=203" rel="nofollow">cellulases;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=oil palm frond&option=203" rel="nofollow">oil palm frond;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=response surface methodology&option=203" rel="nofollow">response surface methodology;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=xylanase&option=203" rel="nofollow">xylanase;</a> </p> <div class="translation"> 机译：灰霉病;纤维素酶;油棕榈叶;反应表面方法;木聚糖酶; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-foreign_renewable-energy_thesis/0204120236186.html">Optimization studies on cellulase and xylanase production by Rhizopus oryzae UC2 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Krisztina Kovacs</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=George Szakacs&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,George Szakacs</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lew Christopher&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Lew Christopher</a> <span> <a href="/journal-cn-7268/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 浙江大学学报（农业与生命科学版） </a> </span> <span> . 2004</span><span>,第004期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201297953618.html">Optimization of the Chitinase Production by Different Metarhizium anisopliae Strains under Solid-State Fermentation with Silkworm Chrysalis as Substrate Using CCRD</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Cynthia Barbosa Rustiguel&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 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Sarat Babu Imandi</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sita Kumari Karanam&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Sita Kumari Karanam</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hanumantha Rao Garapati&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Hanumantha Rao Garapati</a> <span> <a href="/journal-cn-56588/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 生命科学与技术进展（英文） </a> </span> <span> . 2010</span><span>,第2期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201297044540.html">Optimization of Nutrients in Fermentative Lactic Acid Production Using Oil Palm Trunk Juice as Substrate</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nisa Saelee&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . Nisa Saelee</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Klanarong Sriroth&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Klanarong Sriroth</a> <span> <a href="/journal-cn-56588/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 生命科学与技术进展（英文） </a> </span> <span> . 2014</span><span>,第12期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_agricultural-science-technology_thesis/0201273984195.html">Production of Lipase Using Cassava Peel and Sunflower Oil in Solid-State Fermentation： Preliminary Study</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Caroline Branco Gerber&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . Caroline Branco Gerber</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Francieli Kaufmann&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Francieli Kaufmann</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gabrieli Nicoletti&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Gabrieli Nicoletti</a> <span> <a href="/journal-cn-54032/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 农业科学与技术：A </a> </span> <span> . 2013</span><span>,第012期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-19543_thesis/020222240335.html">Optimizing fermentation strategies for production of biosurfactants utilized in oil bioremediation</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wen-Fu Xin&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . Wen-Fu Xin</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yu-Hong Wei&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Yu-Hong Wei</a> <span> <a href="/conference-cn-19543/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 第五届全国化工年会 </a> <span> <span> . 2008</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06130416609226.html">STRAIN OF THE MYCELIAL FUNGUS Of Penicillium of verruculosum- THE PRODUCER OF COMPLEX OF CARBOHYDRASES, ENRICHED the Endo pyrrolidone -1.3/1.4-GLUCANASE AND OF COMPRISING - GLUCOSIDASE, CELLULASE and XYLANASE, AND METHOD OF OBTAINING COMPLEX FERMENTATION PREPARATION FOR USE AS A STERN ADDITIVES</a> <b>[P]</b> . <span> 外国专利： <!-- 俄罗斯专利： --> RU2012139860A </span> <span> . 2014-03-27</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：疣状青霉菌的真菌病原菌-碳水化合物酶复合物的生产者，富含内吡咯烷酮-1.3 / 1.4-葡聚糖酶和包含-葡糖苷酶，纤维素酶和木聚糖酶，并获得了复杂的发酵制剂作为制备方法 </span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06130405781892.html">STRAIN OF TRICHODERMA LONGIBRACHIATUM TW-14-220 FILAMENTOUS FUNGUS - PRODUCER OF CELLULASES, BETA-GLUCANASES AND XYLANASES FOR FEED PRODUCTION AND A METHOD FOR OBTAINING A FEED COMPLEX ENZYME PREPARATION</a> <b>[P]</b> . <span> 外国专利： <!-- 俄罗斯专利： --> RU2654564C1 </span> <span> . 2018-05-21</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：毛滴虫Trichoderma longibrachiatum TW-14-220丝状真菌-用于饲料生产的纤维素酶，β-葡聚糖酶和木聚糖酶生产者以及获得饲料复合酶的方法 </span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130431752099.html">MYCELIAL FUNGUS PENICILLIUM FUNICULOSUM STRAIN AS PRODUCER OF CARBOHYDRASES COMPLEX CONTAINING CELLULASES, -GLUCANASES, -GLUCOSIDASES, XYLANASES AND XYLOGLUCANASES AND METHOD FOR PREPARING ENZYME PREPARATION OF CARBOHYDRASES COMPLEX FOR SACCHARIFICATION OF LIGNOCELULOSE MATERIALS</a> <b>[P]</b> . <span> 外国专利： <!-- 俄罗斯专利： --> RU2323254C2 </span> <span> . 2008-04-27</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：真菌真菌青霉菌镰刀菌菌株，是包含纤维素酶，-葡糖酶，-葡糖苷酶，木聚糖酶和木糖葡聚糖酶的碳水化合物复合物的生产者，以及制备用于酶解的糖酶复合糖蛋白的方法 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" 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