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Case study: The characterization and implementation of dielectric spectroscopy (biocapacitance) for process control in a commercial GMP CHO manufacturing process

机译:案例研究:商业GMP COM制造过程中介电光谱(生物侵蚀率)的表征和实施

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Abstract >Dielectric spectroscopy (biocapacitance) is an up‐and‐coming technology for real time monitoring of biomass in cell culture processes and has opened the door for next‐generation cell culture process control techniques such as automated on‐demand nutrient feeding. In this case study we empirically demonstrate the lower limit of quantitation (LOQ), probe‐to‐probe consistency, and scalability of in situ biocapacitance probes using data generated from small‐ and large‐scale Chinese hamster ovary (CHO) bioreactor cultures. The process understanding experiments culminated in the use of biocapacitance for process control in the current good manufacturing practices (GMP) manufacturing environment, first to automate the dilution of seed train cultures during scale‐up stages and later as a method of predicting future glucose demand. The automated biomass‐probe‐based inoculation strategy yielded consistent results in six consecutive seed trains in the GMP manufacturing suite. In the process of improving our understanding of the technology we determined that biocapacitance could additionally be used as an indicator of a shift in the salt balance of a cell culture, and that collecting real time biomass data via biocapacitance has the potential to reduce the total timeline for feed strategy development by providing additional insights into culture performance which are not otherwise apparent using conventional optical cell counting methods. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译:</span><Abstract Type =“Main”XML:Lang =“en”> <标题类型=“main”>抽象</ title> >介电光谱(生物侵蚀率)是一种即将到来的技术,用于在细胞培养过程中实时监测生物量,并为下一代细胞培养过程控制技术打开了门,例如自动按需营养饲料。在这种情况下,我们经验证明了定量的下限(LOQ),探针探测级度和使用从小型和大规​​模的汉姆斯卵巢(CHO)生物反应器培养物产生的数据的原位生物侵蚀性探针的可扩展性。该过程理解实验在目前良好的制造实践(GMP)制造环境中使用生物侵害的生物侵蚀性,首先在扩大阶段稀释稀释种子列车培养,并作为预测未来血糖需求的方法。自动化生物量探针的接种策略在GMP制造套件中六个连续的种子列车产生一致的结果。在提高我们对技术的理解过程中,我们确定的是生物侵蚀性可以另外用作细胞培养物的盐平衡的转变的指标,并且通过生物侵蚀性收集实时生物量数据有可能减少总时间表用于进料策略开发,通过提供涉及培养性能的额外洞察,所述培养性能与常规光学电池计数方法没有另外明显。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15005/'>《Biotechnology Progress》</a> <b style="margin: 0 2px;">|</b><span>2019年第3期</span><b style="margin: 0 2px;">|</b><span>共11页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Moore Brandon&option=202" target="_blank" rel="nofollow">Moore Brandon;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sanford Ryan&option=202" target="_blank" rel="nofollow">Sanford Ryan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang An&option=202" target="_blank" rel="nofollow">Zhang An;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Cell Culture DevelopmentBiogen Research Triangle ParkNorth Carolina;</p> <p>Cell Culture DevelopmentBiogen Research Triangle ParkNorth Carolina;</p> <p>Cell Culture DevelopmentBiogen Research Triangle ParkNorth Carolina;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/15.html" title="生物科学">生物科学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> </p> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_particuology_thesis/0201249092637.html">Ultrasonic attenuation spectroscopy for multivariate statistical process control in nanomaterial processing</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bundit Boonkhao&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Bundit Boonkhao,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xue Z. 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Kannan摘要的分子系统PDF与文本 DOI 10.17148 / IARJSET.2016.3302在飞机维修中的人类因素Suhas H Begur,J Ashok Babu摘要博士 PDF与文本 DOI 10.17148 / IARJSET.2016.3303人类因素在飞机维修SUHAS H Begur,J Ashok Babu摘要摘要 PDF与文本 DOI 10.17148 / IARJSET.2016.3304叶面植物和抗氧化剂的Diospyros Lancefolia Roxb。 (eBenaceae) - 印度Assam的重要药用植物Dipjyoti Kalita,N. Devi和D.Baishya摘要 PDF与文本 DOI 10.17148 / IARJSET.2016.3305离子迁移性特性和某些电化学综合的多滤网的形态的研究Danesh Roudini,Peter J. S. Stock PDF与文本 DOI 10.17148 / IARJSET.2016.3306人工池塘的物理化学表征控制富营养化过程:一个案例研究同样的Al-Asheh,Hani Abu Qdais,Adnan Alquraishi,Osama Husain,Ismail Sadoon Abstract PDF与文本 DOI 10.17148 / IARJSET.2016.3307调查显示:Web门户网站推荐系统使用客户细分Neha Badami,vipul Wakkar,Monica Jain,Devendra Pandit摘要 PDF与文本 DOI 10.17148 / IARJSET.2016.3308 Web归档:过去的现状和不断发展的多媒体遗产Meenakshi Srivastava,S.K. Singh,S.Q博士。 ABBAS摘要 PDF与文本 DOI 10.17148 / IARJSET.2016.3309劳动合同管理系统Kajol Bhutada,Ketaki Kivade,Vishakha Gokhalale,Pallavi Bhore,Shiv Prasad P. Putil摘要 PDF与文本 DOI 10.17148 / IARJSET.2016.310使用模糊逻辑控制器P.RAMESH BABU,S.RAMPRASATH,N.Vijayasarath,N.VijayasArathi摘要,最小化扭矩纹波和三相感应电动机直接扭矩控制的多象限操作。 PDF与文本 DOI 10.17148 / IARJSET.2016.3311提醒我:实时视频监控系统实施物联网D.P Gaikwad,Pooja Kumawat,Saurabh Bhalerao,Akhilesh Khalate,Hrishikesh Dongre摘要 PDF与文本 DOI 10.17148 / IARJSET.2016.3312 AMAIB录取测试的有效性,可靠性和物品分析林纳·卡卢格博士和Danilo A. Tabalan摘要 PDF与文本 DOI 10.17148 / IARJSET.2016.3313高速通信轨道和保持电路的设计与分析SMITA D. Waghmare,U. A. Kshirsagar摘要 PDF与文本 DOI 10.17148 / IARJSET.2016.3314使用CMOS Technology Nikita V. Dhomane的低功耗数字操作电压调节器设计,Dhomane,U. A. Kshirsagar摘要 PDF与文本 DOI 10.17148 / IARJSET.2016.3315配给分配系统RAJESH B.Shinde,A.G.GAIKWAD教授,Sonali Chincholikar教授摘要 PDF与文本 DOI 10.17148 / IARJSET.2016.3316使用MNSO4污泥作为水泥的局部替代品在混凝土戈霍尔ankush,Jogdand Mohini,Malvi Ketan,Salunke Swanand,Gorade Swapnil摘要 PDF与文本 DOI 10.17148 / IARJSET.2016.3317 Zhanthasagaram Mandal,Nellore District,Andhra Pradesh,India K. Sasdhar,P. Brahmajiroao和A.苏公爵Kumar摘要PDF与文本 DOI 10.17148 / IARJSET.2016.318土壤结构互动对多层建筑苏里亚TEJA CH,SAI KIRAN T摘要的楼层横向位移PDF与文本 DOI 10.17148 / IARJSET.2016.3311概述NARCHEPSY TOUTEEF RAHMAN,OMER FAROOK,MD BELAL BIN HEYAT,MOHD Maroof Siddiqui摘要 PDF与文本 DOI 10.17148 / IARJSET.2016.330在教育建筑中热舒适性的空气运动的意义,案例研究麦德鲁普里亚,Nagaraju Kaja摘要 PDF与文本 DOI 10.17148 / IARJSET.2016.3321一种负载均衡方法,以最大限度地减少云计算Sachin Soni,前列yadav摘要的资源浪费 PDF与文本 DOI 10.17148 / IARJSET.2016.3322 Chickpea S.N流化床干燥的建模与仿真萨哈,G.P.德湾,R.S. Thakur摘要 PDF与文本 DOI 10.17148 / IARJSET.2016.3323光催化纺织染料废水采用固定催化剂系统Rajendiran S,Shriram B,Kanmani S摘要 PDF与文本 DOI 10.17148 / IARJSET.2016.3324网眼较少分析正弦偏斜板在正弦线载荷kumari shipra suman,jeeoot singh摘要 PDF与文本 DOI 10.17148 / IARJSET.2016.325 2 * 2双频宽带圆形贴片天线阵列P. Sai Vinay Kumar,P.Jagadamba,M. N.Giri Prasad摘要摘要 PDF与文本 DOI 10.17148 / IARJSET.2016.3326一种解决云安全问题的多云方法:Qumar M.V,Poornima A. S Abstract PDF与文本 DOI 10.17148 / IARJSET.2016.3327锅炉厂具有不同GCV和碳百分比的升高效率。 P. Bhatt,C.P. Panchal摘要 PDF与文本 DOI 10.17148 / IARJSET.2016.3328工业自动化使用基于传感的应用程序的东西,Geething Chaudhari,Sudarshan Jadhav,Sandeep Batule,Sandeep Helkar摘要 PDF与文本 DOI 10.17148 / IARJSET.2016.3329工程学生学习的评估</span> </p> </li> <li> <div> <b>13. </b><a class="enjiyixqcontent" href="/ntis-science-report_de_thesis/02071957405.html">Laser absorption spectroscopy system for vaporization process characterization and control.</a> <b>[R] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Galkowski, J.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Galkowski, J.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext= Hagans, K.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> Hagans, K. </a> <span>1993</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译:用于汽化过程表征和控制的激光吸收光谱系统。</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-27671_thesis/020221869040.html">Research On Management Pattern for Process Industry In Computer Integrated Manufacturing System</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang Yuan&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . Wang Yuan</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ji GuoLi&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Ji GuoLi</a> <span> <a href="/conference-cn-27671/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 2004中国(福州)国际电子商务学术交流大会暨2004中国国际电子商务高级学术论坛、中国国际电子商务高等教育论坛、中国国际电子商务立法研究学术论坛、中国国际电子商务应用技术研究高级论坛 </a> <span> <span> . 2004</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020315245309.html">Implementation of Process Management in manufacturing industries:A comparative study on manufacturing industries in South Sudan and China</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Atem David DAU&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . Atem David DAU</a> <span> . 2019</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06130479683195.html">electro chemical process characterization through pores of a dielectric covering a metal surface, and device for implementing the process</a> <b>[P]</b> . <span> 外国专利: <!-- 德国专利: --> DE2106593C3 </span> <span> . 1979-01-18</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译:通过覆盖金属表面的电介质的孔进行电化学过程表征以及用于实现该过程的装置 </span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06130480742504.html">electro chemical process characterization through pores of a dielectric covering a metal surface, and device for implementing the process</a> <b>[P]</b> . <span> 外国专利: <!-- 德国专利: --> DE2106593B2 </span> <span> . 1978-05-18</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译:通过覆盖金属表面的电介质的孔进行电化学过程表征以及用于实现该过程的装置 </span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130486836101.html">electro chemical process characterization through pores of a dielectric covering a metal surface, and device for implementing the process</a> <b>[P]</b> . <span> 外国专利: <!-- 瑞士专利: --> CH520331A </span> <span> . 1972-03-15</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译:通过覆盖金属表面的电介质的孔进行电化学过程表征以及用于实现该过程的装置 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon 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