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首页> 外文期刊>Biotechnology Progress >Polyunsaturated fatty acids production by solid-state fermentation on polyurethane foam by Mortierella alpina
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Polyunsaturated fatty acids production by solid-state fermentation on polyurethane foam by Mortierella alpina

机译：Mortierella Alpina的固态发酵产生多不饱和脂肪酸

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摘要

Polyunsaturated fatty acids (PUFAs) are essential in healthy diets and their production is extremely important. Natural sources of PUFAs includes animal and aquatic products such as marine fish oil, however there are several limitations such as the decrease of fish stocks throughout the world. Thus, microbial oils are a preferable source of PUFAs. Herein, it was studied the production of PUFAs by Mortierella alpina under solid-state fermentation (SSF) using polyurethane foam as inert substrate and synthetic medium or lignocellulosic hydrolysate as source of C, N, and other nutrients. Several parameters of fermentation conditions were evaluated as carbon source, inductors addition, ratio C/N and temperature. The highest amount of total PUFAs per mass of solid (535.41 +/- 24.12 mg/g), linoleic acid (129.66 +/- 5.84 mg/g), and alpha-linoleic acid (401.93 +/- 18.10 mg/g) were produced when the culture medium contained 20 g/L glucose, 10% (w/v) linseed oil, the C/N ratio was adjusted to 25 and the incubation temperature was 25 degrees C for 3 days decreasing to 16 degrees C on the remaining 4 days of fermentation. In addition, a hemicellulosic hydrolysate can be used as low-cost substrate to produce PUFAs, although the production was lower than the achieved with synthetic medium. SSF showed an interesting technology for microbial PUFAs production.
机译：多不饱和脂肪酸（PUFA）在健康饮食中至关重要，其生产非常重要。 Pufas的自然来源包括诸如海洋鱼油等动物和水产品，但是有几个局限性，如全世界的鱼类股减少。因此，微生物油是Pufas的优选来源。在此，通过用聚氨酯泡沫作为惰性底物和合成培养基或木质纤维素水解产物作为C，N和其他营养素来源，在固态发酵（SSF）下由Martierella Alpina的生产在固态发酵（SSF）下进行Pufas的生产。评价几个发酵条件参数作为碳源，引物加成，比率C / N和温度。每种固体的最高PUFA（535.41 +/- 24.12 mg / g），亚油酸（129.66 +/- 5.84 mg / g）和α-亚油酸（401.93 +/- 18.10 mg / g）当培养基含有20g / l葡萄糖时，将10％（w / v）亚麻籽油，将C / N比调节至25，孵育温度为25℃，剩余持续16℃下降至16℃ 4天发酵。此外，半纤维素水解产物可用作低成本基质以产生PUFA，尽管生产低于用合成培养基的实现。 SSF为微生物PUFA生产显示了一个有趣的技术。

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