> Present study used Aspergillus terreus strain C1 isolated from mangrove soil for itaconic acid (IA) production from potato starch waste. Fermen'/> Optimization and scale up of itaconic acid production from potato starch waste in stirred tank bioreactor
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Optimization and scale up of itaconic acid production from potato starch waste in stirred tank bioreactor

机译：搅拌釜生物反应器马铃薯淀粉废物的优化与扩大衣康酸生产

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> Present study used Aspergillus terreus strain C1 isolated from mangrove soil for itaconic acid (IA) production from potato starch waste. Fermentation parameters were optimized by classical one factor approach and statistical experimental designs, such as Plackett‐Burman and response surface designs. Anionic deionization of potato waste was found to be a very effective, economic, and easy way of improving IA production. The increase in IA production by deionization was found to correlate with removal of phosphate. In our knowledge, this is the first report on application of deionization of potato waste to enhance IA production. Other parameters like inoculum development conditions, pH, presence of peptone and certain salts in the medium also significantly affected IA production. IA production by strain C1 increased 143‐fold during optimization when compared with the starting condition. The optimized IA level (35.75?g/L) was very close to the maximum production predicted by RSM (38.88?g/L). Bench scale production of IA was further optimized in 3‐L stirred tank reactor by varying parameters like agitation and aeration rate. The maximum IA production of 29.69?g/L was obtained under the agitation speed of 200?rpm and aeration rate of 0.25 vvm. To the best of our knowledge, it is the first report on IA production from potato starch waste at bioreactor level. ? 2019 American Institute of Chemical Engineers Biotechnol. Prog ., 35: e2774, 2019.
机译： > 目前的研究用法从红树林中分离的叶片Terreus菌株C1从马铃薯淀粉废物中脱辣椒酸（IA）生产。通过经典的一种因子方法和统计实验设计优化了发酵参数，例如Plackett-Burman和响应表面设计。发现土豆废物的阴离子去离子是一种非常有效，经济，简单的改善IA生产的方法。发现通过去离子产生的含量的增加与除去磷酸盐相关。在我们的知识中，这是第一份关于将马铃薯废物的去离子应用于增强IA生产的报告。其他参数如接种症状，pH，蛋白胨的存在和培养基中的某些盐也显着影响了IA生产。与起始条件相比，在优化期间，菌株C1的产生增加143倍。优化的IA水平（35.75？G / L）非常接近RSM预测的最大产量（38.88？G / L）。通过改变像搅拌和通气率的参数，在3-L搅拌罐反应器中进一步优化IA的替补尺寸。在200μm的搅拌速度和0.25 VVM的通气率为0.25 VVM的搅拌速度下获得29.69μm的最大产量。据我们所知，这是生物反应器水平的马铃薯淀粉废物中的第一个关于IA生产的报告。还2019年美国化学工程研究所 生物技术。 PROG 。，35：E2774,2019。

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