A peroxidase coordinating to Zn (II) preventing heme bleaching and resistant to the interference of H2O2

Fu Yaqi; Jiang Zhengfeng; Feng Wei

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A peroxidase coordinating to Zn (II) preventing heme bleaching and resistant to the interference of H2O2

机译：将Zn（II）的过氧化物酶坐标，防止血红素漂白和抗干扰H2O2的干扰

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Dehaloperoxidase (DHP) catalyzes detoxifying halophenols. It is a heme-containing enzyme using H(2)O(2)as the oxidant. Heme bleaching from the active site is of great concern. In addition, the interference of DHP by H(2)O(2)leads to the inactivation of the enzyme. To solve these two problems, DHP is coordinated to Zn (II) in PBS buffer to form a biomineralized composite (DHP&Zn-CP). DHP&Zn-CP was characterized by measuring SEM and confocal images, as well as energy dispersive X-ray spectrometry mapping. Fluorescence spectra demonstrated that DHP&Zn-CP can prevent heme bleaching. Two-dimensional FTIR spectra were measured, dynamically providing insight into the structural change of DHP along the coordination process. Raman spectra were performed to analyze the structural change. The optical spectra confirmed that the forming of DHP&Zn-CP had a little effect on the structures of DHP. For the dehalogenation of 2,4,6-trichlorophenol, DHP&Zn-CP can tolerate the presence of H(2)O(2)and is resistant to the interference by H2O2. The catalytic efficiency of DHP&Zn-CP is much higher than that of free DHP.

机译：除山氧化酶（DHP）催化解毒卤代酚。它是使用H（2）O（2）作为氧化剂的含血红素的酶。从活跃点漂白的血红素漂白是非常关注的。另外，通过H（2）O（2）的DHP的干扰导致酶的失活。为了解决这两个问题，DHP与PBS缓冲液中的Zn（II）配位，形成生物丙原化复合物（DHP＆Zn-CP）。 DHP＆Zn-CP的特征在于测量SEM和共聚焦图像，以及能量分散X射线光谱法图。荧光光谱表明DHP＆Zn-CP可以防止血红素漂白。测量二维FTIR光谱，动态地沿着协调过程沿着DHP的结构变化的洞察。进行拉曼光谱以分析结构变化。光谱证实，DHP＆Zn-CP的形成对DHP的结构有点影响。对于2,4,6-三氯化酚苯酚的脱卤，DHP＆Zn-CP可以耐受H（2）O（2）的存在，并且对H 2 O 2的干扰耐干扰。 DHP＆Zn-Cp的催化效率远高于免费的DHP。

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《Biotechnology Progress》 |2021年第1期|共10页
作者
Fu Yaqi; Jiang Zhengfeng; Feng Wei;
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Beijing Univ Chem Technol Dept Biol Engn Beijing 100029 Peoples R China;

Beijing Univ Chem Technol Dept Biol Engn Beijing 100029 Peoples R China;

Beijing Univ Chem Technol Dept Biol Engn Beijing 100029 Peoples R China;

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正文语种 eng
中图分类生物科学;
关键词
coordination; dehaloperoxidase; H2O2; halophenol; heme bleaching;

机译：协调;除山氧化酶;H2O2;卤代苯酚;血红素漂白;

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A peroxidase coordinating to Zn (II) preventing heme bleaching and resistant to the interference of H2O2

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