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Mid‐manufacturing storage: Antibody stability after chromatography and precipitation based capture steps

机译：中间制造储存：色谱分离后的抗体稳定性和基于沉淀的捕获步骤

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Abstract >Antibodies of the IgG2 subclass were captured from the clarified cell culture fluid either by protein A chromatography or by polyethylene glycol precipitation. The captured intermediates were stored as neutralized eluates (protein A chromatography) or in solid form as polyethylene glycol precipitates over a period of 13?months at three temperatures, ?20°C, 5°C, and room temperature to compare the capture technologies in regard of the resulting product storability. Monomer content, high molecular mass impurities product loss and changes in the composition of the charge variants were determined at six time points during the storage. At the beginning and end of the study, samples were additionally tested by differential scanning calorimetry, differential scanning fluorimetry, and circular dichroism to determine structural alterations occurring during storage. Protein A purified material was highly stable at all tested temperatures in regard of monomer content and product losses. A transient, acidic isoform was formed during the chromatography step which re‐converted to the main charged variant upon storage within a matter of days. Precipitated antibodies could be stored at ?20 or 5°C for 3?months without product losses but afterwards recovery yields dropped to 65%. At room temperature, the precipitated antibody was not stable and degraded within 3 months. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> 通过蛋白质A色谱或通过聚乙二醇沉淀，从澄清的细胞培养液中捕获IgG2亚类的抗体。捕获的中间体被储存为中和的洗脱液（蛋白质A色谱）或以固体形式储存，因为聚乙二醇在三个温度下的13个月内沉淀出13℃，Δ20℃，5°C和室温，以比较捕获技术鉴于所产生的产品储存性。单体含量，高分子质量杂质产物损失和电荷变体组合物的变化在储存期间六个时间点测定。在研究的开始和结束时，通过差示扫描量热法，差示扫描荧光测定法和圆形二色性循环测试样品以确定在储存期间发生的结构改变。在单体含量和产物损失方面，蛋白质纯化材料在所有测试温度下高度稳定。在色谱步骤期间形成瞬态酸性同种型，该色谱步骤在储存时重新转化为主电荷变体在几天内。沉淀的抗体可以储存在α0或5℃下3℃，没有产品损失，但后续恢复产量下降至65％。在室温下，沉淀的抗体在3个月内不稳定并降解。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15005/'>《Biotechnology Progress》</a> <b style="margin: 0 2px;">|</b><span>2020年第2期</span><b style="margin: 0 2px;">|</b><span>共11页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Krepper Walpurga&option=202" target="_blank" rel="nofollow">Krepper Walpurga;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Burgstaller Daniel&option=202" target="_blank" rel="nofollow">Burgstaller Daniel;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jungbauer Alois&option=202" target="_blank" rel="nofollow">Jungbauer Alois;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Satzer Peter&option=202" target="_blank" rel="nofollow">Satzer Peter;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of BiotechnologyUniversity of Natural Resources and Life SciencesVienna Austria;</p> <p>Department of BiotechnologyUniversity of Natural Resources and Life SciencesVienna Austria;</p> <p>Department of BiotechnologyUniversity of Natural Resources and Life SciencesVienna Austria;</p> <p>Department of BiotechnologyUniversity of Natural Resources and Life SciencesVienna Austria;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/15.html" title="生物科学">生物科学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=chromatography&option=203" rel="nofollow">chromatography;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=immunoglobulin&option=203" rel="nofollow">immunoglobulin;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=precipitation&option=203" rel="nofollow">precipitation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=storability&option=203" rel="nofollow">storability;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=storage&option=203" rel="nofollow">storage;</a> </p> <div class="translation"> 机译：色谱法;免疫球蛋白;降水;储存性;储存; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-integrative-agriculture_thesis/020126186004.html">Strategies for increasing the capture, storage, and utilization of precipitation in semiarid regions</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=B A Stewart&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">B A Stewart,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=LIANG Wei-li&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">LIANG Wei-li </a> <a href="/journal-foreign-7366/" target="_blank" rel="nofollow" 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B.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Coppock, M. B.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext= Farrow, B.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> Farrow, B.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext= Warner, C.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> Warner, C.,</a> <span>2014</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于肽的蛋白质捕获剂具有高亲和力，选择性和稳定性，作为生物检测分析中的抗体替代品。</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-journal-rare-earths-english_thesis/0201297178063.html">Phase Structure and Cycle Stabilities of Mg_2Ni/Mm_(0.3)Ml_(0.7)Ni_(3.55)Co_(0.75)Mn_(0.4)Al_(0.3) Composite Hydrogen Storage Alloys Prepared by Two-step re-melting</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=黄丽宏&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 黄丽宏</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘向东&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,刘向东</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=闫淑芳&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,闫淑芳</a> <span> <a href="/journal-cn-52787/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 稀土学报：英文版 </a> </span> <span> . 2007</span><span>,第S2期</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06130453695205.html">please keep the stability of a single composition, enrichment, the manufacturing method and cathode precipitation - dip coating to be made in his use of (SINGLE - COMPONENT CONCENTRATE WHICH IS STABLE DURING STORAGE, ITS PRODUCTION and USE IN PREPARING CATHODIC - PRECIPITATION ELETCRO - DIPCOAT BATHS)</a> <b>[P]</b> . <span> 外国专利：  KR987000382A </span> <span> . 1998-03-30</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：请保持单一组成，浓缩，制造方法和阴极沉淀的稳定性-他在使用（单组分浓缩液的过程中要稳定地储存，生产和在制备阴极漆中使用-沉淀电沉积-双涂层） BATHS） </span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06130432832305.html">method of separating one or more antibodies from one or more other compounds in a liquid sample, kits for purifying antibodies of one or more other components in a liquid, and for capturing antibodies from one or more other components in a liquid , chromatography column, and disposable filter for antibody purification</a> <b>[P]</b> . <span> 外国专利：  BRPI0518207A </span> <span> . 2008-11-04</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：从液体样品中的一种或多种其他化合物中分离一种或多种抗体的方法，用于纯化液体中一种或多种其他组分的抗体以及从液体中的一种或多种其他组分中捕获抗体的试剂盒，色谱柱和一次性过滤器用于抗体纯化 </span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130443538108.html">Preparing human immunoglobulin concentrate, useful e.g. for treating infections, by precipitation of plasma then single anion-exchange chromatography step</a> <b>[P]</b> . <span> 外国专利：  FR2824568A1 </span> <span> . 2002-11-15</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：制备人免疫球蛋白浓缩物，例如用于治疗感染，方法是先沉淀血浆，然后再进行阴离子交换层析 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div 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