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Devices and techniques used to obtain and analyze three-dimensional cell cultures

机译：用于获得和分析三维细胞培养物的装置和技术

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摘要

Cell cultures are indispensable for both basic and applied research. Advancements in cell culture and analysis increase their utility for basic research and translational applications. A marked development in this direction is advent of three-dimensional (3D) cultures. The extent of advancement in 3D cell culture methods over the past decade has warranted referring to a single cell type being cultured as an aggregate or spheroid using simple scaffolds as "traditional." In recent years, the development of "next-generation" devices has enabled cultured cells to mimic their natural environments much better than the traditional 3D culture systems. Automated platforms like chip-based devices, magnetic- and acoustics-based assembly devices, di-electrophoresis (DEP), micro pocket cultures (MPoC), and 3D bio-printing provide a dynamic environment compared to the rather static conditions of the traditional simple scaffold-based 3D cultures. Chip-based technologies, which are centered on principles of microfluidics, are revolutionizing the ways in which cell culture and analysis can be compacted into table-top instruments. A parallel evolution in analytical devices enabled efficient assessment of various complex physiological and pathological endpoints. This is augmented by concurrent development of software enabling rapid large-scale automated data acquisition and analysis like image cytometry, elastography, optical coherence tomography, surface-enhanced Raman scattering (SERS), and biosensors. The techniques and devices utilized for the purpose of 3D cell culture and subsequent analysis depend primarily on the requirement of the study. We present here an in-depth account of the devices for obtaining and analyzing 3D cell cultures.
机译：细胞培养对于基础和应用研究是必不可少的。细胞培养和分析的进步增加了他们对基础研究和翻译应用的效用。这个方向的明显发展是三维（3D）培养的出现。在过去十年中，3D细胞培养方法的进步程度是指用作培养为骨料或球形的单细胞类型，使用简单的支架为“传统”。近年来，“下一代”设备的发展使培养细胞能够比传统的3D培养系统更好地模仿它们的自然环境。基于芯片的设备，磁性和声学的组装装置，二电泳（DEP），微口袋培养物（MPOC）等自动化平台，与传统简单的相当静态条件相比，提供了一种动态环境。基于脚手架的3D文化。基于芯片的技术，以微流体的原则为中心，正在彻底改变细胞培养和分析可以压制成桌面仪器的方式。分析装置中的平行进化使得能够有效评估各种复杂的生理和病理终点。这是通过软件的并发开发来增强快速大规模自动数据采集和分析，如图像细胞计，弹性造影，光学相干断层扫描，表面增强拉曼散射（SERS）和生物传感器。用于3D细胞培养的目的和随后分析的技术和装置主要取决于研究的要求。我们在此提供了用于获得和分析3D细胞培养的设备的深入叙述。

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