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首页> 外文期刊>Biotechnology Progress >The effect of acetate on population heterogeneity in different cellular characteristics of Escherichia coli in aerobic batch cultures
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The effect of acetate on population heterogeneity in different cellular characteristics of Escherichia coli in aerobic batch cultures

机译：有氧批量培养物中大肠杆菌不同细胞特征中醋酸血液异质性的影响

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Abstract > Acetate as the major by‐product in industrial‐scale bioprocesses with <fc> <fi>Escherichia coli</fi> </fc> is found to decrease process efficiency as well as to be toxic to cells, which has several effects like a significant induction of cellular stress responses. However, the underlying phenomena are poorly explored. Therefore, we studied time‐resolved population heterogeneity of the <fc> <fi>E. coli</fi> </fc> growth reporter strain MG1655/pGS20P rrnB GFPAAV expressing destabilized green fluorescent protein during batch growth on acetate and glucose as sole carbon sources. Additionally, we applied five fluorescent stains targeting different cellular properties (viability as well as metabolic and respiratory activity). Quantitative analysis of flow cytometry data verified that bacterial populations in the bioreactor are more heterogeneous in growth as well as stronger metabolically challenged during growth on acetate as sole carbon source, compared to growth on glucose or acetate after diauxic shift. Interestingly, with acetate as sole carbon source, significant subpopulations were found with some cells that seem to be more robust than the rest of the population. In conclusion, following batch cultures population heterogeneity was evident in all measured parameters. Our approach enabled a deeper study of heterogeneity during growth on the favored substrate glucose as well as on the toxic by‐product acetate. Using a combination of activity fluorescent dyes proved to be an accurate and fast alternative as well as a supplement to the use of a reporter strain. However, the choice of combination of stains should be well considered depending on which population traits to aim for. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 作为工业规模生物过程中的主要副产物 <FC> <fi>大肠杆菌</ fi> </ FC> 发现降低过程效率以及对细胞有毒，其具有几种效果，如细胞应激反应的显着诱导。然而，潜在的现象探索着差不多。因此，我们研究了时间分辨的人口异质性 <FC> <fi> e。 COLI </ FI> </ FC> 生长记者菌株MG1655 / PGS20P rrnb </ i> GFPAAV在乙酸盐和葡萄糖的批量生产期间表达了不稳定的绿色荧光蛋白作为唯一碳源。另外，我们施加了靶向不同细胞性质的五种荧光污渍（可行性以及代谢性和呼吸活动）。流式细胞术数据的定量分析验证了生物反应器中的细菌群体在生长中更为异质，并且在乙酸盐的生长期间与唯一碳源的生长期间更强的代谢攻击，与杂糖叶片葡萄糖或乙酸盐的生长相比。有趣的是，用醋酸盐作为唯一的碳源，发现有显着的亚群与一些似乎比其余人口更强大的细胞。总之，在批量培养物之后，在所有测量的参数中都显而易见。我们的方法使得在有利的底物葡萄糖以及毒性乙酸盐上的生长期间能够更深入研究异质性。使用活性荧光染料的组合被证明是准确和快速的替代方案以及使用报告菌株的补充剂。然而，根据瞄准的人口特征，应考虑污渍组合的选择。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15005/'>《Biotechnology Progress》</a> <b style="margin: 0 2px;">|</b><span>2019年第3期</span><b style="margin: 0 2px;">|</b><span>共14页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Heins Anna‐Lena&option=202" target="_blank" rel="nofollow">Heins Anna‐Lena;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lundin Luisa&option=202" target="_blank" rel="nofollow">Lundin Luisa;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nunes Inês&option=202" target="_blank" rel="nofollow">Nunes Inês;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gernaey Krist V.&option=202" target="_blank" rel="nofollow">Gernaey Krist V.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=S?rensen S?ren J.&option=202" target="_blank" rel="nofollow">S?rensen S?ren J.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lantz Anna Eliasson&option=202" target="_blank" rel="nofollow">Lantz Anna Eliasson;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Chemical and Biochemical EngineeringTechnical University of DenmarkKongens Lyngby Denmark;</p> <p>Department of Biology Section of MicrobiologyUniversity of CopenhagenCopenhagen Denmark;</p> <p>Department of Biology Section of MicrobiologyUniversity of CopenhagenCopenhagen Denmark;</p> <p>Department of Chemical and Biochemical EngineeringTechnical University of DenmarkKongens Lyngby Denmark;</p> <p>Department of Biology Section of MicrobiologyUniversity of CopenhagenCopenhagen Denmark;</p> <p>Department of Chemical and Biochemical EngineeringTechnical University of DenmarkKongens Lyngby Denmark;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/15.html" title="生物科学">生物科学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=flow cytometry&option=203" rel="nofollow">flow cytometry;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=growth on acetate&option=203" rel="nofollow">growth on acetate;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=metabolic activity&option=203" rel="nofollow">metabolic activity;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=population heterogeneity&option=203" rel="nofollow">population heterogeneity;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=reporter strain&option=203" rel="nofollow">reporter strain;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=viability&option=203" rel="nofollow">viability;</a> </p> <div class="translation"> 机译：流式细胞术;醋酸盐生长;代谢活动;人口异质性;记者菌株;可行性; </div> </li> </ul> </div> </div> <div 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</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：通过分批分批培养将乙酸香叶酯生物转化为香叶醇的工程化大肠杆菌可高产香叶醇</span> </p> </li> <li> <div> <b>11. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/01000131804079.html">The effect of acetate on population heterogeneity in different cellular characteristics ofEscherichia coliin aerobic batch cultures</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Anna‐Lena Heins&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Anna‐Lena Heins,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Luisa Lundin&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Luisa Lundin,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Inês Nunes&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Inês Nunes,</a> <span>2019</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：醋酸盐对不同细胞特征群体异质性的影响，Coliin Aerobic分批培养物不同细胞特征</span> </p> </li> <li> <div> <b>12. </b><a class="enjiyixqcontent" href="/ntis-science-report_pb_thesis/020711230710.html">Disinfection Resistance of 'Legionella pneumophila' and 'Escherichia coli' Grown in Continuous and Batch Culture</a> <b>[R] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hoff, J. C. &option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Hoff, J. C. ,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Roberts, P. V. &option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Roberts, P. V. ,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Matin, A. &option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Matin, A. ,</a> <span>1985</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：连续和分批培养的'嗜肺军团菌'和'大肠杆菌'的消毒能力</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_academic-abstracts-chemical-engineering-chinese-colleges-universities_thesis/0201249304526.html">The rpoS deficiency suppresses acetate accumulation in glucose-enriched culture of Escherichia coli under an aerobic condition</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Prayoga SURYADARMA&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . Prayoga SURYADARMA</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yoshihiro OJIMA&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Yoshihiro OJIMA</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yuto FUKUDA&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Yuto FUKUDA</a> <span> <a href="/journal-cn-9263/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国高等学校学术文摘·化学工程 </a> </span> <span> . 2012</span><span>,第002期</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06130412056835.html">ESCHERICHIA COLI STRAIN WITH MUTATIONS FOR THE HIGH PRODUCTION OF RECOMBINANT NUCLEIC ACIDS WITH A LOW ACCUMULATION OF ACETATE AND THE USE THEREOF IN CULTURES OF HIGH MOLECULAR DENSITY IN BATCH MODE.</a> <b>[P]</b> . <span> 外国专利： <!-- --> MX342245B </span> <span> . 2016-09-14</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：带有突变的大肠埃希氏菌以高产量生产重组核酸且乙酸盐含量低，并且在批量模式下在高分子密度培养中的应用。 </span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06130420289536.html">ESCHERICHIA COLI STRAIN WITH MUTATIONS FOR THE HIGH PRODUCTION OF RECOMBINANT NUCLEIC ACIDS WITH A LOW ACCUMULATION OF ACETATE AND THE USE THEREOF IN CULTURES OF HIGH MOLECULAR DENSITY IN BATCH MODE.</a> <b>[P]</b> . <span> 外国专利： <!-- --> MX2011013392A </span> <span> . 2013-06-17</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：带有突变的大肠埃希氏菌菌株，用于以低积累乙酸盐的方式高产量生产重组核酸，并用于批量生产高分子密度的文化。 </span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130442509342.html">Vaccine composition, use of enteroemorrhagic escherichia coli cell culture supernatant (ehec), and methods for generating an immune response in a mammal against a secreted enteroemorrhagic escherichia coli (ehec) antigen, and enteroemorrhagic 0157: h7 (ehec 0157) : h7) secreted, and for reduction of colonization of enteroemorrhagic escherichia coli (ehec) in a ruminant</a> <b>[P]</b> . <span> 外国专利： <!-- --> BR0206312A </span> <span> . 2004-02-17</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：疫苗的组成，肠出血性大肠杆菌细胞培养上清液（ehec）的使用以及在哺乳动物中产生针对分泌型肠出血性大肠杆菌（ehec）抗原的免疫反应以及分泌的肠出血性0157：h7（ehec 0157）：h7）的方法，和减少反刍动物肠出血性大肠杆菌（ehec）的定殖 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 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