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首页> 外文期刊>Biotechnology Journal: Healthcare,Nutrition,Technology >Naming CHO cells for bio-manufacturing: Genome plasticity and variant phenotypes of cell populations in bioreactors question the relevance of old names
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Naming CHO cells for bio-manufacturing: Genome plasticity and variant phenotypes of cell populations in bioreactors question the relevance of old names

机译：用于生物制造的命名CHO细胞：生物反应器中细胞群的基因组可塑性和变异表型质疑旧名称的相关性

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摘要

Chinese Hamster Ovary [CHO] cells are the workhorse for production of modern biopharmaceuticals. They are however immortalized cells with a high propensity for genetic change. Judging from published culture records, CHO cell populations have undergone hundreds of population doublings since their origin in the late 1950s. Different cell populations were established and named from 1 to 3 decades after their generation, such as CHO-Pro-, CHO-K1, CHO-DG44, CHO-S, CHO-DUK, CHO-DXB-11 to indicate origin and certain phenotypic features. These names are commonly used in scientific publications still today. This article discusses the relevance of such names. We argue that they provide a false sense of identity. To substantiate this, we provide the long (and poorly recorded) history of CHO cells as well as their highly complex genetics. Finally, we suggest an alternative naming system for CHO cells which provides more relevant information. While the implementation of a new naming convention will require substantial discussions among members of the relevant community, it should improve interpretation and comparability between laboratories. This, in turn will help scientific communities and industrial users to attain and further the full potential of CHO cells.
机译：中国仓鼠卵巢[CHO]细胞是制作现代生物制药的主力。然而，它们是永生化的细胞，具有高遗传变化倾向。从发表的文化记录中判断，由于20世纪50年代后期，Cho Cell opmulations自从他们的起源以来经历了数百人口倍增。在他们的生成之后建立和命名为不同的细胞群，例如Cho-Pro-，Cho-K1，Cho-DG44，Cho-S，Cho-Duk，Cho-DXB-11表示起源和某些表型特征。这些名称常用于今天的科学出版物。本文讨论了此类名称的相关性。我们认为他们提供了一个虚假的身份感。为了证实这一点，我们提供Cho细胞的长（录制且较差）的历史以及它们的高度复杂的遗传学。最后，我们建议为CHO单元提供替代命名系统，其提供更多相关信息。虽然新的命名公约的实施将需要有关社区成员之间的大量讨论，但它应该改善实验室之间的解释和可比性。这反过来，这将有助于科学的社区和工业用户获得和进一步的愈合细胞的全部潜力。

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