...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Journal: Healthcare,Nutrition,Technology >Biotechnological production of the European corn borer sex pheromone in the yeast Yarrowia lipolytica
【24h】

Biotechnological production of the European corn borer sex pheromone in the yeast Yarrowia lipolytica

机译：欧洲玉米螟的生物技术生产在酵母Yarrowia Lipolytica酵母中的玉米螟性信息素

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The European corn borer (ECB) Ostrinia nubilalis is a widespread pest of cereals, particularly maize. Mating disruption with the sex pheromone is a potentially attractive method for managing this pest; however, chemical synthesis of pheromones requires expensive starting materials and catalysts and generates hazardous waste. The goal of this study was to develop a biotechnological method for the production of ECB sex pheromone. Our approach was to engineer the oleaginous yeast Yarrowia lipolytica to produce (Z)-11-tetradecenol (Z11-14:OH), which can then be chemically acetylated to (Z)-11-tetradecenyl acetate (Z11-14:OAc), the main pheromone component of the Z-race of O. nubilalis. First, a C14 platform strain with increased biosynthesis of myristoyl-CoA was obtained by introducing a point mutation into the alpha-subunit of fatty acid synthase, replacing isoleucine 1220 with phenylalanine (Fas2p(I1220F)). The intracellular accumulation of myristic acid increased 8.4-fold. Next, fatty acyl-CoA desaturases (FAD) and fatty acyl-CoA reductases (FAR) from nine different species of Lepidoptera were screened in the C14 platform strain, individually and in combinations. A titer of 29.2 +/- 1.6 mg L-1 Z11-14:OH was reached in small-scale cultivation with an optimal combination of a FAD (Lbo_PPTQ) from Lobesia botrana and FAR (HarFAR) from Helicoverpa armigera. When the second copies of FAD and FAR genes were introduced, the titer improved 2.1-fold. The native FAS1 gene's overexpression led to a further 1.5-fold titer increase, reaching 93.9 +/- 11.7 mg L-1 in small-scale cultivation. When the same engineered strain was cultivated in controlled 1 L bioreactors in fed-batch mode, 188.1 +/- 13.4 mg L-1 of Z11-14:OH was obtained. Fatty alcohols were extracted from the biomass and chemically acetylated to obtain Z11-14:OAc. Electroantennogram experiments showed that males of the Z-race of O. nubilalis were responsive to biologically-derived pheromone blend. Behavioral bioassays in a wind tunnel revealed attraction of male O. nubilalis, although full precopulatory behavior was observed less often than for the chemically synthesized pheromone blend. The study paves the way for the production of ECB pheromone by fermentation.
机译：欧洲玉米螟（欧洲贺卡）Ostrinia Nubilalis是一种普遍的谷物谷物，特别是玉米。与性信息素交配中断是管理这个害虫的潜在有吸引力的方法;然而，信息素的化学合成需要昂贵的原料和催化剂并产生危险废物。本研究的目标是为生产ECB性信息素的生物技术方法进行生物技术方法。我们的方法是将糖胺酵母Yarrowia Lipolytica工程生产（Z）-11-四苯酚（Z11-14：OH），然后可以将其化学乙酰化至（Z）-11-四烯基乙酸（Z11-14：OAC）， O. Nubilalis的Z比的主要信息素组分。首先，通过将点突变引入脂肪酸合成酶的α-亚基，用苯丙氨酸（FAS2P（I1220F））替代异氨酸1220来获得具有MyRistoyl-CoA的生物合成的C14平台菌株。肉豆蔻酸的细胞内积累增加了8.4倍。接下来，在C14平台菌株，单独和组合中筛选脂肪酰基-CoA去饱和酶（FAD）和脂肪酰基-COA还原酶（FAT）和脂肪酰基-COA还原酶（FAT）。滴度为29.2 +/- 1.6 mg L-1 Z11-14：OH是以小规模培养达到的，具有来自Helicoverpa Armigera的Lobesia Botrana和Fax（Harfar）的FAD（LBO_PPTQ）的最佳组合。当引入时的第二份和远基因的第二份拷贝时，滴度提高了2.1倍。天然FAS1基因的过度表达导致进一步的1.5倍滴度增加，达到93.9 +/- 11.7mg L-1，小规模栽培。当在加入批量模式的控制1L生物反应器中培养相同的工程菌株时，获得Z11-14的188.1 +/- 13.4mg L-1：OH。从生物质中萃取脂肪醇并在化学上乙酰化以获得Z11-14：OAC。电器环图实验表明，O. Nubilalis的Z比的雄性对生物衍生的信息素共混物反应。风洞中的行为生物测定揭示了雄性O. Nubilalis的吸引力，尽管较少观察到较少的预备性行为而不是化学合成的信息素共混物。该研究铺平了通过发酵生产欧洲央行信息素的方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号