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首页> 外文期刊>Biomedical Chromatography: An International Journal Devoted to Research in Chromatographic Methodologies and Their Applications in the Biosciences >Determination of free clindamycin, flucloxacillin or tedizolid in plasma: Pay attention to physiological conditions when using ultrafiltration
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Determination of free clindamycin, flucloxacillin or tedizolid in plasma: Pay attention to physiological conditions when using ultrafiltration

机译：测定自由克林霉素，氟氯苄嘧啶或泰迪唑啉在血浆中的注意力：使用超滤时注意生理条件

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摘要

Pharmacokinetic/pharmacodynamic indices of anti-infective drugs should be referenced to free drug concentrations. In the present study, clindamycin, flucloxacillin and tedizolid have been determined in human plasma by HPLC-UV. The drugs were separated isocratically within 3-6 min on a C_18 column using mixtures of phosphate buffer-acetonitrile of pH 7.1-7.2. Sample treatment for the determination of total drug concentrations in plasma included extraction/back-extraction (clindamycin) or protein precipitation (flucloxacillin, tedizolid). The free drug concentrations were determined after ultrafiltration. An ultrafiltration device with a membrane consisting of regenerated cellulose proved to be suitable for all drugs. Maintaining a physiological pH was crucial for clindamycin, whereas maintaining body temperature was essential for tedizolid. The methods were applied to the analysis of total and free drug concentrations in clinical samples and were sufficiently sensitive for pharmacokinetic studies and therapeutic drug monitoring.
机译：应参考抗感染性药物的药代动力学/药效学索引免受游离药物浓度。在本研究中，通过HPLC-UV在人血浆中测定了Clindamycin，Flycloxacillin和Tedizolid。使用pH7.1-7.2的磷酸盐缓冲液的混合物，在C_18柱上在3-6分钟内分离各自的药物。用于测定血浆中总药物浓度的样品处理包括萃取/后萃取（Clindamycin）或蛋白质沉淀（氟氯苄三种蛋白，泰迪唑啉）。超滤后测定游离药物浓度。具有由再生纤维素组成的膜的超滤装置被证明适用于所有药物。保持生理pH对于克林霉素至关重要，而维持体温对于泰迪唑啉是必不可少的。将该方法应用于临床样品中总量和游离药物浓度的分析，对药代动力学研究和治疗药物监测足够敏感。

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