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首页> 外文期刊>Biodegradation >Biodegradation of petroleum refining industry oil sludge by microbial-assisted biocarrier matrix: process optimization using response surface methodology
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Biodegradation of petroleum refining industry oil sludge by microbial-assisted biocarrier matrix: process optimization using response surface methodology

机译：微生物辅助生物围流基质的石油精炼工业油污泥生物降解：使用响应面法测定过程优化

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Safe disposal of petroleum oil sludge generated from crude oil storage tank bottom is a major challenge for petroleum refineries across the globe. The presence of long chain hydrocarbons in petroleum oil sludge are known to have effects on the environment through bioaccumulation or biosorption. The present study was focused to develop a modified bioremediation process using hydrocarbonoclastic microbial-assisted biocarrier matrix (MABC) mediated through biosurfactants and biocatalysts for the efficient treatment of petroleum industrial oily sludge. The development of hydrocarbonoclastic microbial-assisted biocarrier matrix was confirmed by scanning electron microscopy analysis. The biocatalysts such as lipase, laccase, esterase and biosurfactant produced by MABC system were found to be 40 U/mg, 18 U/mg, 36 U/mg and 220 mg/g of oil sludge respectively using one variable at a time approach. Further, the response surface methodology was used to determine the optimum treatment conditions (Time, pH, Mass of biocarrier matrix and Amount of oil sludge) for the enhanced removal of total petroleum hydrocarbons (TPH) present in the oil sludge and TPH was degraded by 88.78% at Hydraulic Retention Time of 7 days. The biodegradation of oil sludge was confirmed using Gas Chromatography-Mass Spectrometry analysis.
机译：安全处理原油储罐底部产生的石油污泥是全球石油炼油厂的主要挑战。已知通过生物累积或生物吸附对环境长链碳氢化合物的存在。本研究的重点是使用通过生物表面活性剂和生物催化剂介导的烃类细胞癌微生物辅助生物载体基质（MABC）来开发改性的生物化方法，用于高效地处理石油工业油性污泥。通过扫描电子显微镜分析证实了烃类细胞型微生物辅助生物载波基质的发展。通过MABC系统产生的脂肪酶，漆酶，酯酶和生物活性剂等生物催化剂被发现为40u / mg，18U / mg，36U / mg和220mg / g分别使用一个变量以时间方法使用一个变量。此外，响应表面方法用于确定用于在油污泥中存在的总石油烃（TPH）的增强除去的最佳处理条件（时间，pH，生物载体基质和油污泥量的量）液压保留时间为7天的88.78％。使用气相色谱 - 质谱分析证实了油污泥的生物降解。

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