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首页> 外文期刊>British Poultry Science >Recombinant lactobacillin PlnK adjusts the gut microbiome distribution in broilers
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Recombinant lactobacillin PlnK adjusts the gut microbiome distribution in broilers

机译：重组乳酸杆菌PLNK调节肉鸡中的肠道微生物组分布

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1. The heterologous expression and biological function of the Lactobacillus bacteriocin plantaricin K (PlnK) remain largely unknown. 2. In this study, PlnK was efficiently expressed in competentE. coliBL21 (used in transformation and protein expression) after 12 h, at 37 degrees C and in 0.4 mmol/l isopropyl beta- d-1-thiogalactopyranoside (IPTG). 3. The inhibitory bacterial spectrum of recombinant PlnK was investigated and indicated that levels of PlnK above 0.10 mg/ml produced an obvious inhibitory effect on gram-positive bacteria and gram-negative bacteriain vitro. 4. The effects of PlnK on intestinal immune function and the gut microbiome distribution in broilers were studied. The results revealed that, after consuming 2.50 x 10(3) mg/ml of PlnK in water for one week, at the phylum level, the abundance ofFirmicuteswas increased and the abundance ofBacleroideteswas decreased. At the family level, the abundance of Lachnospiraceae, Ruminococcaceae and Streptococcaceae were significantly improved, but the abundance of Bacteroidaceae was reduced. At the genus level, the abundances ofLachnoclostridium, StreptococcusandRuminococcaceae-UCG-013, were significantly up-regulated, and the abundance ofBacteroideswas down-regulated. 5. After oral liquid intake of PlnK for one week, levels of secretory immunoglobulin A (sIgA) in the duodenal mucus were not significantly increased, but the mRNA levels ofTLR3,MDA5,IFN-alpha, IFN-beta, IFITM3 andIFITM10 in the duodenum were significantly reduced. 6. This study demonstrated that the recombinant PlnK could adjust the intestinal microbiome distribution and downregulate the IFN pathway.
机译：1.乳酸杆菌菌株植物蛋白K（PLNK）的异源表达和生物学功能仍然很大程度上是未知的。在本研究中，PLNK有效地表达于竞争力。在12小时后的ColiBl21（用于转化和蛋白质表达），在37℃和0.4mmol / L异丙基β-D-1-硫酰基吡喃糖苷（IPTG）。 3.研究了重组PLNK的抑制细菌谱，并表明PLNK水平以上0.10mg / mL对革兰氏阳性细菌和革兰氏阴性杀菌素体外产生明显的抑制作用。 4.研究了PLNK对肠道免疫功能的影响和肉鸡肠道微生物组分布。结果表明，在水中消耗2.50×10（3）毫克/ mL / mL的PLNK之后，在门槛水平上，丰富的Defirmicutesswas增加，丰富的Bacleroidetesswas降低。在家庭层面，大量的Lachnospiraceae，喇叭杆菌菌和链球菌显着改善，但抗菌痤疮的丰度降低。在属级，洛氏细胞肺炎链球菌的丰度被显着上调，高度调节的亚替葡萄球菌的丰度。 5.在口腔液体摄入量1周后，十二指肠粘液中分泌免疫球蛋白A（SIGA）的水平未显着增加，但是在十二指肠中的第三，MDA5，IFN-α，IFN-Beta，IFITM3 Andifitm10的mRNA水平显着减少了。本研究表明，重组PLNK可以调节肠道微生物组分布并下调IFN途径。

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