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首页> 外文期刊>Biochemistry and molecular biology education >Simple and rapid system for two-dimensional gel electrophoresis technique: A laboratory exercise for high school students
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Simple and rapid system for two-dimensional gel electrophoresis technique: A laboratory exercise for high school students

机译：二维凝胶电泳技术简单快捷的系统：高中生的实验室运动

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摘要

Polyacrylamide gel electrophoresis (PAGE) is the most classical technique favored worldwide for resolution of macromolecules in many biochemistry laboratories due to its incessant advanced developments and wide modifications. These ever-growing advancements in the basic laboratory equipments lead to emergence of many expensive, complex, and tricky laboratory equipments. Practical courses of biochemistry at high school or undergraduate levels are often affected by these complications. Two dimensional gel electrophoresis technique (2D-PAGE) used for resolving thousands of proteins in a gel is a combination of isoelectric focusing (first dimension gel electrophoresis technique) and sodium-dodecylsulphate PAGE (second dimension gel electrophoresis technique or SDS-PAGE). Two different laboratory equipments are needed to carry out effective 2D-PAGE technique, which also invites extra burden to the school laboratory. Here, we describe a low cost, time saving and simple gel cassette for protein 2D-PAGE technique that uses easily fabricated components and routine off-the-shelf materials. The performance of the apparatus was verified in a practical exercise by a group of high school students with positive outcomes. (c) 2018 by The International Union of Biochemistry and Molecular Biology, 46(3):237-244, 2018.
机译：聚丙烯酰胺凝胶电泳（PAGE）是最古典的技术，用于解决许多生物化学实验室中的大分子，由于其不断的先进发展和宽泛的修改。基本实验室设备的这些越来越长的进步导致了许多昂贵，复杂和棘手的实验室设备的出现。高中或本科水平的实际生物化学课程往往受这些并发症的影响。用于在凝胶中解析成千上万蛋白质的二维凝胶电泳技术（2D-PAGE）是等电聚焦（第一尺寸凝胶电泳技术）和钠 - 十二烷基硫酸盐页面（第二尺寸凝胶电泳技术或SDS-PAGE）的组合。需要两种不同的实验室设备来执行有效的2D页技术，这也邀请了学校实验室的额外负担。在这里，我们描述了用于蛋白质2D-PAGE技术的低成本，时空和简单的凝胶盒，其使用易于制造的部件和常规现成的材料。该装置的性能在一群高中学生的实际运动中验证了一群积极的结果。 （c）2018由国际生物化学联盟和分子生物学，46（3）：237-244,2018。

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