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首页> 外文期刊>Cell biology international. >The optimization conditions of establishing an H9c2 cardiomyocyte hypoxia/reoxygenation injury model based on an AnaeroPack System
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The optimization conditions of establishing an H9c2 cardiomyocyte hypoxia/reoxygenation injury model based on an AnaeroPack System

机译：基于Anaeropack系统建立H9C2心肌细胞缺氧/雷诺损伤模型的优化条件

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摘要

Ischemia-reperfusion (I/R) injury is a major cause of cardiomyocyte apoptosis after vascular recanalization, which was mimicked by a hypoxia/reoxygenation (H/R) injury model of cardiomyocytes in vitro. In this study, we explored an optimal H/R duration procedure using the AnaeroPack System. To study the H/R procedure, cardiomyocytes were exposed to the AnaeroPack System with sugar and serum-free medium, followed by reoxygenation under normal conditions. Cell injury was detected through lactate dehydrogenase (LDH) and cardiac troponin (c-Tn) release, morphological changes, cell apoptosis, and expression of apoptosis-related proteins. The results showed that the damage to H9c2 cells increased with prolonged hypoxia time, as demonstrated by increased apoptosis rate, LDH and c-Tn release, HIF-1 alpha expression, as well as decreased expression of Bcl-2. Furthermore, hypoxia for 10 h and reoxygenation for 6 h exhibited the highest apoptosis rate and damage and cytokine release; in addition, cells were deformed, small, and visibly round. After 12 h of hypoxia, the majority of the cells were dead. Taken together, this study showed that subjecting H9c2 cells to the AnaeroPack System for 10 h and reoxygenation for 6 h can achieve a practicable and repeatable H/R injury model.
机译：缺血再灌注（I/R）损伤是血管再通后心肌细胞凋亡的主要原因，体外心肌细胞缺氧/复氧（H/R）损伤模型对此进行了模拟。在这项研究中，我们使用Anaropack系统探索了最佳的H/R持续时间程序。为了研究H/R程序，将心肌细胞暴露于含有糖和无血清培养基的厌氧回输系统中，然后在正常条件下进行复氧。通过乳酸脱氢酶（LDH）和心肌肌钙蛋白（c-Tn）的释放、形态学变化、细胞凋亡和凋亡相关蛋白的表达检测细胞损伤。结果表明，随着缺氧时间的延长，H9c2细胞的损伤程度增加，表现为凋亡率增加、LDH和c-Tn释放增加、HIF-1α表达增加以及Bcl-2表达降低。缺氧10h和复氧6h的细胞凋亡率、损伤和细胞因子释放最高；此外，细胞变形，小，可见圆形。缺氧12小时后，大部分细胞死亡。综上所述，这项研究表明，将H9c2细胞置于厌氧回输系统中10小时，再给氧6小时，可以获得一个实用且可重复的h/R损伤模型。

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  • 来源
    《Cell biology international.》 |2021年第4期|共9页
  • 作者

    Wen Jingyi; Wang Dan; Cheng Lichun; Wu Di; Qiu Lulu; Li Miao; Xie Yu; Wu Si; Jiang Yan; Bai Hansheng; Xu Bing; Lv Huiyi;

  • 作者单位

    Dalian Med Univ Affiliated Hosp 2 Dept Pharm Dalian 116023 Liaoning Peoples R China;

    Dalian Med Univ Affiliated Hosp 2 Dept Pharm Dalian 116023 Liaoning Peoples R China;

    Dalian Med Univ Affiliated Hosp 2 Dept Pharm Dalian 116023 Liaoning Peoples R China;

    Dalian Med Univ Affiliated Hosp 2 Dept Pharm Dalian 116023 Liaoning Peoples R China;

    Dalian Med Univ Affiliated Hosp 2 Dept Pharm Dalian 116023 Liaoning Peoples R China;

    Dalian Med Univ Affiliated Hosp 2 Dept Pharm Dalian 116023 Liaoning Peoples R China;

    Dalian Med Univ Affiliated Hosp 2 Dept Pharm Dalian 116023 Liaoning Peoples R China;

    Dalian Med Univ Affiliated Hosp 2 Dept Pharm Dalian 116023 Liaoning Peoples R China;

    Dalian Med Univ Affiliated Hosp 2 Dept Pharm Dalian 116023 Liaoning Peoples R China;

    Dalian Med Univ Affiliated Hosp 2 Dept Pharm Dalian 116023 Liaoning Peoples R China;

    Dalian Med Univ Affiliated Hosp 2 Dept Pharm Dalian 116023 Liaoning Peoples R China;

    Dalian Med Univ Affiliated Hosp 2 Dept Pharm Dalian 116023 Liaoning Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    AnaeroPack System; apoptosis; cardiomyocytes; hypoxia; reoxygenation injury model; ischemiaamp; 8211; reperfusion injury;

    机译：Anaeropack系统;细胞凋亡;心肌细胞;缺氧;Reoxygenation损伤模型;缺血＆amp;8211;再灌注损伤;

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