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首页> 外文期刊>Cell and Tissue Research >MiR-150-5p regulate T cell activation in severe aplastic anemia by targeting Bach2
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MiR-150-5p regulate T cell activation in severe aplastic anemia by targeting Bach2

机译：miR-150-5p通过靶向bach2调节严重的血栓性贫血中的T细胞活化

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摘要

MiR-150-5p is an immune-related miRNA and elevated in the plasma of patients with aplastic anemia (AA), but its role in T cell activation in patients with severe aplastic anemia (SAA) is unclear. This study aims to explore the role of miR-150-5p in T cell activation of SAA. RT-PCR and Western blot were used to detect the expression of mRNA and protein. The cell proportion was detected by flow cytometry. The lentiviruses expressing miR-150-5p inhibitor and Bach2 shRNA were respectively infected to produce stable miR-150-5p or Bach2 knockout cells. Brdu incorporation method was used to detect T cell proliferation. SAA mouse model was induced with cyclophosphamide and busulfan, and intravenous injection of LV inhibitor NC and LV-miR-150-5p inhibitor. The miR-150-5p expression is up-regulated in SAA, which is negatively correlated with Bach2. Inhibition of miR-150-5p reduces the activation of T cells. MiR-150-5p directly targeted 3 ' UTR of Bach2. Moreover, the expression of miR-150-5p and the activation of T cells were decreased in SAA mouse model. MiR-150-5p promotes T cell activation in SAA by targeting Bach2. Targeting miR-150-5p may be a new molecular therapy for SAA patients.
机译：MiR-150-5p是一种免疫相关的miRNA，在再生障碍性贫血（AA）患者血浆中升高，但其在严重再生障碍性贫血（SAA）患者T细胞活化中的作用尚不清楚。本研究旨在探讨miR-150-5p在SAA T细胞活化中的作用。RT-PCR和westernblot检测mRNA和蛋白的表达。流式细胞仪检测细胞比例。分别感染表达miR-150-5p抑制剂和Bach2 shRNA的慢病毒以产生稳定的miR-150-5p或Bach2敲除细胞。Brdu掺入法检测T细胞增殖。用环磷酰胺和白消安诱导SAA小鼠模型，静脉注射LV抑制剂NC和LV-miR-150-5p抑制剂。miR-150-5p在SAA中表达上调，与Bach2呈负相关。抑制miR-150-5p可降低T细胞的激活。MiR-150-5p直接针对Bach2的3'UTR。此外，在SAA小鼠模型中，miR-150-5p的表达和T细胞的激活降低。MiR-150-5p通过靶向Bach2促进SAA中的T细胞活化。靶向miR-150-5p可能是治疗SAA患者的一种新的分子疗法。

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  • 来源
    《Cell and Tissue Research》 |2021年第2期|共12页
  • 作者

    Li Yingmei; Yu Jifeng; Wang Fang; Guo Rong; Xing Haizhou; Chen Yali; Chen Dandan; Xie Xinsheng; Wan Dingming; Jiang Zhongxing;

  • 作者单位

    Zhengzhou Univ Affiliated Hosp 1 Dept Hematol Zhengzhou 450052 Henan Peoples R China;

    Zhengzhou Univ Affiliated Hosp 1 Dept Hematol Zhengzhou 450052 Henan Peoples R China;

    Zhengzhou Univ Affiliated Hosp 1 Dept Hematol Zhengzhou 450052 Henan Peoples R China;

    Zhengzhou Univ Affiliated Hosp 1 Dept Hematol Zhengzhou 450052 Henan Peoples R China;

    Zhengzhou Univ Affiliated Hosp 1 Dept Hematol Zhengzhou 450052 Henan Peoples R China;

    Zhengzhou Univ Affiliated Hosp 1 Dept Hematol Zhengzhou 450052 Henan Peoples R China;

    Zhengzhou Univ Affiliated Hosp 1 Dept Hematol Zhengzhou 450052 Henan Peoples R China;

    Zhengzhou Univ Affiliated Hosp 1 Dept Hematol Zhengzhou 450052 Henan Peoples R China;

    Zhengzhou Univ Affiliated Hosp 1 Dept Hematol Zhengzhou 450052 Henan Peoples R China;

    Zhengzhou Univ Affiliated Hosp 1 Dept Hematol Zhengzhou 450052 Henan Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    Severe aplastic anemia; T cell activation; miR-150-5p; Bach2;

    机译：严重的血栓性贫血;T细胞激活;miR-150-5p;bach2;

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