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首页> 外文期刊>Cell and Tissue Research >Kinesin-7 CENP-E regulates the formation and structural maintenance of the acrosome
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Kinesin-7 CENP-E regulates the formation and structural maintenance of the acrosome

机译：Kinesin-7 CENP-E调节了uphosome的形成和结构维持

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摘要

The acrosome is a special organelle that develops from the Golgi apparatus and the endolysosomal compartment in the spermatids. Centromere protein E (CENP-E) is an essential kinesin motor in chromosome congression and alignment. This study is aimed at investigating the roles and mechanisms of kinesin-7 CENP-E in the formation of the acrosome during spermatogenesis. Male ICR mice are injected with GSK923295 for long-term inhibition of CENP-E. Chemical inhibition and siRNA-mediated knockdown of CENP-E are carried out in the GC-2 spd cells. The morphology of the acrosomes is determined by the HE staining, immunofluorescence, and transmission electron microscopy. We have identified CENP-E is a key factor in the formation and structural maintenance of the acrosome during acrosome biogenesis. Long-term inhibition of CENP-E by GSK923295 results in the asymmetric acrosome and the dispersed acrosome. CENP-E depletion leads to the malformation of the Golgi complex and abnormal targeting of the PICK1- and PIST-positive Golgi-associated vesicles. Our findings uncover an essential role of CENP-E in membrane trafficking and structural organization of the acrosome in the spermatids during spermatogenesis. Our results shed light on the molecular mechanisms involved in vesicle trafficking and architecture maintenance of the acrosome.
机译：顶体是一种特殊的细胞器，由精子细胞的高尔基体和内溶酶体室发育而来。着丝粒蛋白E（CENP-E）是一种重要的运动蛋白，参与染色体的整合和排列。本研究旨在探讨驱动蛋白-7 CENP-E在精子发生过程中顶体形成中的作用和机制。雄性ICR小鼠注射GSK923295长期抑制CENP-E。在GC-2 spd细胞中进行化学抑制和siRNA介导的CENP-E敲除。顶体的形态由HE染色、免疫荧光和透射电镜确定。我们已经确定CENP-E是顶体生物发生过程中顶体形成和结构维持的关键因素。GSK923295对CENP-E的长期抑制导致不对称顶体和分散顶体。CENP-E缺失导致高尔基复合体畸形和PICK1和PIST阳性高尔基相关小泡的异常靶向性。我们的发现揭示了CENP-E在精子发生过程中精子细胞顶体的膜运输和结构组织中的重要作用。我们的研究结果揭示了囊泡运输和顶体结构维持的分子机制。

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  • 来源
    《Cell and Tissue Research》 |2021年第3期|共16页
  • 作者

    She Zhen-Yu; Yu Kai-Wei; Wei Ya-Lan; Zhong Ning; Lin Yang;

  • 作者单位

    Fujian Med Univ Sch Basic Med Sci Dept Cell Biol &

    Genet Fuzhou 350122 Fujian Peoples R China;

    Fujian Med Univ Sch Basic Med Sci Dept Cell Biol &

    Genet Fuzhou 350122 Fujian Peoples R China;

    Fujian Obstet &

    Gynecol Hosp Fuzhou 350011 Fujian Peoples R China;

    Fujian Med Univ Sch Basic Med Sci Dept Cell Biol &

    Genet Fuzhou 350122 Fujian Peoples R China;

    Fujian Med Univ Sch Basic Med Sci Dept Cell Biol &

    Genet Fuzhou 350122 Fujian Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    Kinesin-7; CENP-E; Acrosome; The Golgi complex; Spermatogenesis;

    机译：kinesin-7;cenp-e;ugosome;golgi复合物;精子发生;

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