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首页> 外文期刊>Cell and Tissue Research >Prosaposin in the rat oviductal epithelial cells
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Prosaposin in the rat oviductal epithelial cells

机译：大鼠产卵上皮细胞的蛋白酶蛋白酶

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Prosaposin (PSAP) has two forms: a precursor and a secreted form. The secreted form has neurotrophic, myelinotrophic, and myotrophic properties. The precursor form is a precursor protein of saposins A-D. Although the distribution of PSAP in male reproductive organs is well known, its distribution in female reproductive organs, especially in the oviduct, is unclear. Immunoblots and immunohistochemistry of oviducts showed that oviductal tissues contain PSAP proteins, and a significant increase in PSAP was observed in the estrus-metestrus phase compared to the diestrus-proestrus phase in the ampulla. To identify PSAP trafficking in cells, double-immunostaining was performed with antibodies against PSAP in combination with sortilin, mannose 6 phosphate receptor (M6PR), or low-density lipoprotein receptor-related protein 1 (LRP1). PSAP and sortilin double-positive reactions were observed near the nuclei, as well as in the apical portion of microvillous epithelial cells, whereas these reactions were only observed near the nuclei of ciliated epithelial cells. PSAP and M6PR double-positive reactions were observed near the nuclei of microvillous and ciliated epithelial cells. PSAP and M6PR double-positive reactions were also observed in the apical portion of microvillous epithelial cells. PSAP and LRP1 double-positive reactions were observed in the plasma membrane and apical portion of both microvillous and ciliated epithelial cells. Immunoelectron staining revealed PSAP immunoreactive small vesicles with exocytotic features at the apical portion of microvillous epithelial cells. These findings suggest that PSAP is present in the oviductal epithelium and has a pivotal role during pregnancy in providing an optimal environment for gametes and/or sperm in the ampulla.
机译：前载脂蛋白（PSAP）有两种形式：前体形式和分泌形式。分泌型具有神经营养、髓鞘营养和肌营养特性。前体形式是saposins a-D的前体蛋白。虽然已知PSAP在男性生殖器官中的分布，但其在女性生殖器官，尤其是输卵管中的分布尚不清楚。输卵管的免疫印迹和免疫组织化学显示，输卵管组织含有PSAP蛋白，与壶腹发情间期-发情前期相比，在发情中期观察到PSAP显著增加。为了确定细胞中的PSAP转运，使用抗PSAP抗体与sortilin、甘露糖6磷酸受体（M6PR）或低密度脂蛋白受体相关蛋白1（LRP1）联合进行双重免疫染色。PSAP和sortilin双阳性反应在微绒毛上皮细胞的细胞核附近以及顶端部分观察到，而这些反应仅在纤毛上皮细胞的细胞核附近观察到。在微绒毛和纤毛上皮细胞核附近观察到PSAP和M6PR双重阳性反应。在微绒毛上皮细胞顶端也观察到PSAP和M6PR双重阳性反应。在微绒毛和纤毛上皮细胞的质膜和顶端均观察到PSAP和LRP1双重阳性反应。免疫电子染色显示PSAP免疫反应性小泡在微绒毛上皮细胞顶端具有胞吐特征。这些发现表明，PSAP存在于输卵管上皮中，在怀孕期间为壶腹中的配子和/或精子提供最佳环境方面起着关键作用。

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