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首页> 外文期刊>Cell and Tissue Research >Quantitative analysis of enteric neurons containing choline acetyltransferase and nitric oxide synthase immunoreactivities in the submucosal and myenteric plexuses of the porcine colon
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Quantitative analysis of enteric neurons containing choline acetyltransferase and nitric oxide synthase immunoreactivities in the submucosal and myenteric plexuses of the porcine colon

机译：含胆碱乙酰转移酶和一氧化氮合酶免疫功能性的肠道神经元的定量分析和猪结肠的神经丛

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摘要

The enteric nervous system (ENS) controls gastrointestinal functions. In large mammals' intestine, it comprises an inner (ISP) and outer (OSP) submucous plexus and a myenteric plexus (MP). This study quantifies enteric neurons in the ISP, OSP, and MP of the pig ascending (AC) and descending colon (DC) using the HuC/D, choline acetyltransferase (ChAT), and neuronal nitric oxide synthase (nNOS) neuronal markers in whole mount preparations with multiple labeling immunofluorescence. We established that the ISP contains the highest number of HuC/D neurons/mm(2), which were more abundant in AC vs. DC, followed by OSP and MP with similar density in AC and DC. In the ISP, the density of ChAT immunoreactive (IR) neurons was very similar in AC and DC (31% and 35%), nNOS-IR neurons were less abundant in AC than DC (15% vs. 42%,P < 0.001), and ChAT/nNOS-IR neurons were 5% and 10%, respectively. In the OSP, 39-44% of neurons were ChAT-IR in AC and DC, while 45% and 38% were nNOS-IR and 10-12% were ChAT/nNOS-IR (AC vs. DCP < 0.05). In the MP, ChAT-IR neurons were 44% in AC and 54% in DC (P < 0.05), nNOS-IR neurons were 50% in both, and ChAT/nNOS-IR neurons were 12 and 18%, respectively. The ENS architecture with multilayered submucosal plexuses and the distribution of functionally distinct groups of neurons in the pig colon are similar to humans, supporting the suitability of the pig as a model and providing the platform for investigating the mechanisms underlying human colonic diseases.
机译：肠道神经系统（ENS）控制胃肠功能。在大型哺乳动物的肠道中，它由内（ISP）和外（OSP）粘膜下丛和肌间丛（MP）组成。本研究使用HuC/D、胆碱乙酰转移酶（ChAT）和神经元型一氧化氮合酶（nNOS）神经元标记物，采用多重标记免疫荧光法对猪升结肠（AC）和降结肠（DC）的ISP、OSP和MP中的肠道神经元进行定量。我们确定，ISP含有最高数量的HuC/D神经元/mm（2），在AC和DC中更丰富，其次是在AC和DC中密度相似的OSP和MP。在ISP中，ChAT免疫反应（IR）神经元在AC和DC中的密度非常相似（分别为31%和35%），nNOS IR神经元在AC中的含量低于DC（分别为15%和42%，P<0.001），ChAT/nNOS IR神经元分别为5%和10%。在OSP中，39-44%的神经元在AC和DC中为ChAT-IR，45%和38%为nNOS-IR，10-12%为ChAT/nNOS-IR（AC和DCP<0.05）。在MP中，ChAT-IR神经元在AC和DC中分别为44%和54%（P<0.05），nNOS-IR神经元在两者中均为50%，ChAT/nNOS-IR神经元分别为12%和18%。猪结肠内具有多层粘膜下神经丛的ENS结构和功能不同的神经元群的分布与人类相似，支持猪作为模型的适用性，并为研究人类结肠疾病的潜在机制提供了平台。

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