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首页> 外文期刊>Cell cycle >Long non-coding RNA GASL1 restrains gastric carcinoma cell proliferation and metastasis by sponging microRNA-106a
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Long non-coding RNA GASL1 restrains gastric carcinoma cell proliferation and metastasis by sponging microRNA-106a

机译：长期非编码RNA Gasl1通过海绵MicroRNA-106A限制胃癌细胞增殖和转移

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摘要

Background Gastric carcinoma (GC) is a common malignant tumor. Recently, it has been found that long non-coding RNAs (lncRNAs) play important role in cancer. In this paper, we investigated the effects and mechanism of lncRNA GASL1 in GC cells. Methods GASL1 level in GC cells was up-regulated via cell transfection. Cell proliferation, migration, invasion were detected by CCK-8, BrdU, Transwell assays and western blot. In addition, the regulation of GASL1 on microRNA (miR)-106a level was detected using RT-qPCR and the binding between GASL1 and miR-106a was confirmed by bioinformatic prediction and luciferase reporter assay. The effects of overexpressing miR-106a on GASL1-regulated GC cell behaviors were further explored. Moreover, western blot also was used to detect the pathway-related proteins. Results Overexpression of GASL1 decreased the viability and BrdU levels. Meanwhile, CyclinD1 level was decreased while p53 and p21 levels were strengthened by overexpression of GASL1. On cell metastasis, up-regulation of GASL1 decreased cell migration, invasion and related proteins matrix metalloproteinase (MMP)-9 and Vimentin levels. Meanwhile, silencing GASL1 exerted opposite effects on GC cells. Moreover, GASL1 negatively regulated and targeted miR-106a. Up-regulation of miR-106a weakened the functions of GASL1 in cell proliferation and metastasis. Besides, GASL1 decreased the relate-protein levels of PI3K/AKT and ras/raf/MEK/ERK pathways while miR-106a weakened these changes. Conclusion GASL1 restrained GC cell proliferation and metastasis and blocked PI3K/AKT and ras/raf/MEK/ERK pathways by sponging miR-106a.
机译：背景胃癌是一种常见的恶性肿瘤。最近，人们发现长非编码RNA（lncRNAs）在癌症中起着重要作用。本文研究了lncRNA GASL1在GC细胞中的作用及其机制。方法通过细胞转染，使GC细胞中GASL1水平上调。通过CCK-8、BrdU、Transwell分析和western blot检测细胞增殖、迁移和侵袭。此外，使用RT-qPCR检测GASL1对microRNA（miR）-106a水平的调节，并通过生物信息学预测和荧光素酶报告分析确认GASL1和miR-106a之间的结合。进一步探讨了过度表达miR-106a对GASL1调节的GC细胞行为的影响。此外，westernblot也用于检测通路相关蛋白。结果GASL1的过度表达降低了细胞活力和BrdU水平。同时，GASL1的过度表达降低了CyclinD1的水平，而增强了p53和p21的水平。在细胞转移中，GASL1的上调降低了细胞迁移、侵袭及相关蛋白基质金属蛋白酶（MMP）-9和波形蛋白的水平。同时，沉默GASL1对GC细胞产生相反的影响。此外，GASL1对miR-106a具有负调控和靶向性。miR-106a的上调削弱了GASL1在细胞增殖和转移中的功能。此外，GASL1降低了PI3K/AKT和ras/raf/MEK/ERK通路的相关蛋白水平，而miR-106a减弱了这些变化。结论GASL1通过分泌miR-106a抑制GC细胞增殖和转移，阻断PI3K/AKT和ras/raf/MEK/ERK通路。

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  • 来源
    《Cell cycle》 |2020年第20期|共11页
  • 作者

    Liu Dengqiang; Xiao Peng; Feng Chao; Meng Hui; Bi Enxu;

  • 作者单位

    Qingdao West Coast New Area Cent Hosp Dept Gen Surg Qingdao Shandong Peoples R China;

    Qingdao West Coast New Area Cent Hosp Dept Gen Surg Qingdao Shandong Peoples R China;

    Qingdao West Coast New Area Cent Hosp Dept Gen Surg Qingdao Shandong Peoples R China;

    Qingdao West Coast New Area Cent Hosp Dept Gynecol Qingdao Shandong Peoples R China;

    Qingdao West Coast New Area Cent Hosp Dept Gen Surg Qingdao Shandong Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    Gastric carcinoma; proliferation; metastasis; lncRNA; GASL1; miR-106a;

    机译：胃癌;增殖;转移;lncrna;gasl1;mir-106a;

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