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首页> 外文期刊>Cell cycle >Knockdown of circular RNA circMAT2B reduces oxygen-glucose deprivation-induced inflammatory injury in H9c2 cells through up-regulating miR-133
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Knockdown of circular RNA circMAT2B reduces oxygen-glucose deprivation-induced inflammatory injury in H9c2 cells through up-regulating miR-133

机译：圆形RNA循环循环循环率将通过Up-Consemating MiR-133降低H9C2细胞中的氧葡萄糖剥夺诱导炎症损伤

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摘要

Myocardial infarction (MI) is the main cause of morbidity and mortality. Reperfusion ways can cause damage to cardiomyocytes. CircMAT2B, a novel circRNA, takes positive roles in regulating glucose metabolism under hypoxia. Therefore, we aimed to explore the effects of circMAT2B on MI. Oxygen-glucose deprivation (OGD)-induced H9c2 cell model was employed to stimulate MI. Ex-circMAT2B, si-circMAT2B, miR-133 inhibitor and relative control were transfected into H9c2 cells. qRT-PCR was employed to examine levels of circMAT2B and miR-133. Cell activity, apoptosis, ROS generation and release of inflammatory factors were assessed by CCK-8, flow cytometry, ROS species assay kit and ELISA, respectively. Moreover, the expression of apoptosis-related and pathway-related factors was detected through western blot analysis. The results showed that circMAT2B expression was notably up-regulated by OGD treatment. Moreover, circMAT2B knockdown could effectively decrease OGD-induced the increasing of apoptosis, ROS generation and the expression of IL-1 beta, IL-6 and TNF-alpha. Besides, miR-133 was positively regulated by si-circMAT2B. CircMAT2B knockdown attenuated OGD-induced H9c2 cell damage and alleviated OGD-induced the inhibition of PI3K/AKT and Raf/MEK/ERK pathways through up-regulating miR-133. In brief, circMAT2B knockdown works as an inflammatory inhibitor in OGD-induced H9c2 cells inflammatory injury through up-regulating miR-133.
机译：心肌梗死（MI）是发病率和死亡率的主要原因。再灌注方式可导致心肌细胞损伤。CircMAT2B是一种新型的circRNA，在低氧条件下对糖代谢具有积极的调节作用。因此，我们旨在探讨circMAT2B对心肌梗死的影响。采用氧糖剥夺（OGD）诱导的H9c2细胞模型刺激心肌梗死。将Ex-circMAT2B、si-circMAT2B、miR-133抑制剂和相对对照转染H9c2细胞。qRT-PCR检测circMAT2B和miR-133的水平。分别用CCK-8、流式细胞术、活性氧物种检测试剂盒和ELISA检测细胞活性、细胞凋亡、活性氧生成和炎症因子释放。通过westernblot分析检测凋亡相关因子和通路相关因子的表达。结果表明，OGD处理显著上调了circMAT2B的表达。此外，circMAT2B基因敲除可有效降低OGD诱导的细胞凋亡、ROS生成以及IL-1β、IL-6和TNFα表达的增加。此外，miR-133受到si-circMAT2B的正调控。CircMAT2B基因敲除可减轻OGD诱导的H9c2细胞损伤，并通过上调miR-133减轻OGD对PI3K/AKT和Raf/MEK/ERK通路的抑制。简而言之，circMAT2B基因敲除通过上调miR-133在OGD诱导的H9c2细胞炎症损伤中起到炎症抑制剂的作用。

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  • 来源
    《Cell cycle》 |2020年第20期|共9页
  • 作者

    Zhu Yanhui; Zou Chengwei; Jia Yanting; Zhang Haizhou; Ma Xiaochun; Zhang Jun;

  • 作者单位

    Shandong Univ Shandong Prov Hosp Dept Cardiac Surg Jinan Shandong Peoples R China;

    Shandong Univ Shandong Prov Hosp Dept Cardiac Surg Jinan Shandong Peoples R China;

    Shandong Univ Shandong Prov Hosp Dept Cardiac Surg Jinan Shandong Peoples R China;

    Shandong Univ Shandong Prov Hosp Dept Cardiac Surg Jinan Shandong Peoples R China;

    Shandong Univ Shandong Prov Hosp Dept Cardiac Surg Jinan Shandong Peoples R China;

    Shandong First Med Univ Shandong Prov Hosp Dept Cardiac Surg Jinan Shandong Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    Circular RNA circMAT2B; miR-133; oxygen-glucose deprivation; inflammatory injury;

    机译：圆形RNA Circmat2B;miR-133;氧葡萄糖剥夺;炎症损伤;

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