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首页> 外文期刊>Cell cycle >NRF1-regulated CircNSUN2 promotes lymphoma progression through activating Wnt signaling pathway via stabilizing HMGA1
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NRF1-regulated CircNSUN2 promotes lymphoma progression through activating Wnt signaling pathway via stabilizing HMGA1

机译：通过通过稳定HMGA1激活Wnt信号传导途径，NRF1调节的Circnsun2通过激活Wnt信号通路促进淋巴瘤进展

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摘要

Lymphoma is the malignant tumor in the lymphatic system. Circular RNAs (circRNAs) are non-coding RNAs with closed structure, which have been reported to perform critical functions in various tumor progressions. However, the role of circNSUN2 in lymphoma has not been well explored. Quantitative reverse transcription real-time polymerase chain reaction (RT-qPCR) assay was performed to test the expression of circNSUN2 in malignant lymphoma tissues and normal lymph tissues, as well as in human peripheral blood lymphocyte cell line and malignant lymphoma cell lines. Cell counting kit-8 (CCK-8) assay and Transwell assays were used to evaluate the function of circNSUN2 on lymphoma cell proliferation, migration and invasion. DNA pull-down assay, chromatin immunoprecipitation (ChIP) and luciferase reporter assay were employed to test the interaction between circNSUN2 and NRF1. TOP/FOP flash reporter assay was performed to detect influence of circNSUN2 on Wnt pathway. Luciferase reporter assay and RNA pull-down assay were performed to explore interaction between HMGA1 and circNSUN2 through Wnt pathway. CircNSUN2 expression was abnormally high in malignant lymphoma tissues and cell lines. CircNSUN2 inhibition could reduce proliferation and invasion of lymphoma. Bioinformatic analysis, DNA pull-down, ChIP and luciferase reporter experiments confirmed that circNSUN2 could be modulated by transcription factor NRF1. Through RT-qPCR, western blot and luciferase reporter assays, circNSUN2 was proved to influence Wnt pathway by modulating HMGA1. CircNSUN2 regulated by transcription factor NRF1 could promote lymphoma progression through activating Wnt pathway via stabilizing HMGA1.
机译：淋巴瘤是淋巴系统中的恶性肿瘤。环状RNA（CircRNA）是具有封闭结构的非编码RNA，据报道在各种肿瘤进展中发挥关键作用。然而，circNSUN2在淋巴瘤中的作用尚未得到很好的探讨。采用定量逆转录实时聚合酶链反应（RT-qPCR）检测circNSUN2在恶性淋巴瘤组织、正常淋巴组织、人外周血淋巴细胞系和恶性淋巴瘤细胞系中的表达。细胞计数试剂盒-8（CCK-8）分析和Transwell分析用于评估circNSUN2对淋巴瘤细胞增殖、迁移和侵袭的作用。采用DNA下拉试验、染色质免疫沉淀（ChIP）和荧光素酶报告试验检测circNSUN2和NRF1之间的相互作用。通过TOP/FOP flash reporter分析检测circNSUN2对Wnt通路的影响。通过荧光素酶报告分析和RNA下拉分析，探讨HMGA1和circNSUN2通过Wnt途径的相互作用。CircNSUN2在恶性淋巴瘤组织和细胞系中的表达异常高。CircNSUN2抑制可减少淋巴瘤的增殖和侵袭。生物信息学分析、DNA下拉、芯片和荧光素酶报告实验证实，circNSUN2可以被转录因子NRF1调节。通过RT-qPCR、western blot和荧光素酶报告分析，证实circNSUN2通过调节HMGA1影响Wnt通路。由转录因子NRF1调节的CircNSUN2可通过稳定HMGA1激活Wnt通路促进淋巴瘤的进展。

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  • 来源
    《Cell cycle》 |2021年第9期|共10页
  • 作者

    Wang Lifu; Yang Bo; Xu Ziguang; Song Xiaoxia; Gong Zhiquan; Xue Shuang; Kong Lingfei;

  • 作者单位

    Zhengzhou Univ Peoples Hosp Henan Prov Peoples Hosp Dept Pathol Zhengzhou Henan Peoples R;

    Zhengzhou Univ Affiliated Hosp 3 Dept Med Detect Zhengzhou Henan Peoples R China;

    Zhengzhou Univ Peoples Hosp Henan Prov Peoples Hosp Dept Pathol Zhengzhou Henan Peoples R;

    Zhengzhou Univ Peoples Hosp Henan Prov Peoples Hosp Dept Pathol Zhengzhou Henan Peoples R;

    Zhengzhou Univ Peoples Hosp Henan Prov Peoples Hosp Dept Pathol Zhengzhou Henan Peoples R;

    Zhengzhou Univ Peoples Hosp Henan Prov Peoples Hosp Dept Pathol Zhengzhou Henan Peoples R;

    Zhengzhou Univ Peoples Hosp Henan Prov Peoples Hosp Dept Pathol Zhengzhou Henan Peoples R;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    Lymphoma; circNSUN2; NRF1; HMGA1; Wnt pathway;

    机译：淋巴瘤;Circnsun2;NRF1;HMGA1;WNT途径;

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