Three-dimensional bio-printed constructs consisting of human umbilical-derived mesenchymal stem cells promote cell viability, proliferation, and differentiation in vitro

Tao Qingxia; Wu Cuiying; Li Xinda; Chen Wenjin; Sun Kai; Zhang Peng; Yang Zhijun; Liu Ning; Xu Ruxiang; Xu Tao; Wang Chong

首页> 外文期刊>Cellular and molecular biology >Three-dimensional bio-printed constructs consisting of human umbilical-derived mesenchymal stem cells promote cell viability, proliferation, and differentiation in vitro

【24h】

Three-dimensional bio-printed constructs consisting of human umbilical-derived mesenchymal stem cells promote cell viability, proliferation, and differentiation in vitro

机译：由人脐衍生的间充质干细胞组成的三维生物印刷构建体促进细胞活力，增殖和体外分化

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The aim of this study was to investigate the effect of three-dimensional (3D) bio-printed constructs consisting of human umbilical-derived mesenchymal stem cells (HUMSCs) on cell viability, proliferation and differentiation in vitro. Functional 3D bio-printed microspheres consisting of HUMSCs were constructed using electrostatic inkjet technique. The parameters used for the synthesis of 3D bio-printed tissue constructs were first optimized. The viability, proliferation and differentiation of 3D cultured HUMSCs were assessed. The results of scanning electron microscopy (SEM) showed that isolated HUMSCs exhibited fibroblast-like spindle adherent growth. The optimized printing parameters were 6 kV voltage, 10 mL/h flow, 15 cm receiving height, and alginate: water ratio of 1:1 mixed at 37 degrees C. Compared with 2D cultured HUMSCs, the 3D cultured HUMSCs have better viability; proliferation and differentiation ability. The results obtained in this study indicate that 3D bio-printed tissue constructs promote HUMSC viability, proliferation, and neural differentiation in vitro.

机译：本研究的目的是在体外研究由人脐带间充质干细胞（HUMSCs）组成的三维生物打印结构对细胞活力、增殖和分化的影响。利用静电喷墨技术构建了功能性三维生物打印微球。首先优化了用于合成3D生物打印组织结构的参数。对三维培养的人骨髓间充质干细胞的活力、增殖和分化进行了评估。扫描电镜（SEM）结果显示，分离的人骨髓间充质干细胞呈成纤维细胞样纺锤体贴壁生长。最佳打印参数为6kv电压、10ml/h流量、15cm接收高度、37℃下海藻酸钠与水的比例为1:1。与二维培养的人骨髓间充质干细胞相比，三维培养的人骨髓间充质干细胞具有更好的活力；增殖和分化能力。本研究获得的结果表明，3D生物打印组织构建物在体外可促进HUMSC的活性、增殖和神经分化。

著录项

来源
《Cellular and molecular biology》 |2020年第2期|共7页
作者
Tao Qingxia; Wu Cuiying; Li Xinda; Chen Wenjin; Sun Kai; Zhang Peng; Yang Zhijun; Liu Ning; Xu Ruxiang; Xu Tao; Wang Chong;
展开▼
作者单位

Peoples Liberat Army Gen Hosp Med Ctr 7 Affiliated Bayi Brain Hosp Dept Neurosurg Beijing;

Peoples Liberat Army Gen Hosp Med Ctr 7 Affiliated Bayi Brain Hosp Dept Neurosurg Beijing;

Tsinghua Univ Dept Mech Engn Biomfg &

Rapid Forming Technol Key Lab Beijing Beijing 100084;

Southern Med Univ PLA Gen Hosp Med Ctr 7 Dept Neurosurg Affiliated Bayi Brain Hosp Guangzhou;

Army Med Univ PLA Gen Hosp Med Ctr 7 Dept Neurosurg Affiliated Bayi Brain Hosp Chongqing 400038;

Peoples Liberat Army Gen Hosp Med Ctr 7 Affiliated Bayi Brain Hosp Dept Neurosurg Beijing;

Peoples Liberat Army Gen Hosp Med Ctr 7 Affiliated Bayi Brain Hosp Dept Neurosurg Beijing;

Peoples Liberat Army Gen Hosp Med Ctr 7 Affiliated Bayi Brain Hosp Dept Neurosurg Beijing;

Peoples Liberat Army Gen Hosp Med Ctr 7 Affiliated Bayi Brain Hosp Dept Neurosurg Beijing;

Tsinghua Univ Dept Mech Engn Biomfg &

Rapid Forming Technol Key Lab Beijing Beijing 100084;

Shanghai Univ Med &

Hlth Sci Affliated Zhoupu Hosp Shanghai 310115 Peoples R China;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类分子生物学;
关键词
Human umbilical-derived mesenchymal stem cell; Three-dimensional bioprinted microspheres; Viability; Proliferation; Bio-electrospraying;

机译：人脐衍生的间充质干细胞;三维生物印刷的微球;活力;增殖;生物电喷雾;

相似文献

外文文献
中文文献
专利

1. Human bone morphogenetic protein-2 gene transfer induces human mesenchymal stem cell proliferation and differentiation in vitro [J] . 李军, 范清宇, 钱济先, 中国人民解放军军医大学学报（英文版） . 2004,第002期
2. Recombinant human bone morphogenetic protein-2 promotes the proliferation of mesenchymal stem cells in vivo and in vitro [J] . LIU Shui-bing, HU Pei-zhen, HOU Ying, 中华医学杂志（英文版） . 2009,第007期
3. Growth differentiation factor 6,a repressive target of EZH2,promotes the commitment of human embryonic stem cells to mesenchymal stem cells [J] . Pend Deng, Yongxin Yu, Christine Hong, 骨研究：英文 . 2020,第004期
4. Growth differentiation factor 6, a repressive target of EZH2, promotes the commitment of human embryonic stem cells to mesenchymal stem cells [J] . Pend Deng, Yongxin Yu, Christine Hong, 骨研究（英文版） . 2020,第004期
5. 3D bio-printed scaffold-free nerve constructs with human gingiva-derived mesenchymal stem cells promote rat facial nerve regeneration [J] . Qunzhou Zhang, Phuong D. Nguyen, Shihong Shi, Scientific reports. . 2018,第1期

机译：3D生物印刷的支架无神经构建体与人牙龈衍生的间充质干细胞促进大鼠面部神经再生
6. Human amnion mesenchymal stem cells promote proliferation and osteogenic differentiation in human bone marrow mesenchymal stem cells [J] . Wang Yuli, Yin Ying, Jiang Fei, Journal of molecular histology . 2015,第1期

机译：人羊膜间充质干细胞促进人骨髓间充质干细胞的增殖和成骨分化
7. Reconstitution of bone-like matrix in osteogenically differentiated mesenchymal stem cell–collagen constructs: A three-dimensional in vitro model to study hematopoietic stem cell niche [J] . WY Lai, YY Li, SK Mak, Journal of Tissue Engineering . 2013,第1期

机译：骨形成分化的间充质干细胞-胶原构建物中骨样基质的重建：研究造血干细胞生态位的三维体外模型
8. Effects of age on proliferation and osteogenic differentiation of human mesenchymal stem cell in vitro and bone formation in nude mice [C] . Xu Jianzhong The 2nd World Congress for Chinese Biomedical Engineers . 2004

机译：年龄对人间充质干细胞体外增殖和成骨分化以及裸鼠骨形成的影响
9. Evaluating the capacity of adult human mesenchymal stem cells to differentiate into insulin-producing cells in vitro [D] . Ratanshi, Imran 2009

机译：评估成人间充质干细胞体外分化为产生胰岛素的细胞的能力
10. Osteogenic Differentiation of Three-Dimensional Bioprinted Constructs Consisting of Human Adipose-Derived Stem Cells In Vitro and In Vivo [O] . Xiao-Fei Wang, Yang Song, Yun-Song Liu, -1

机译：体外和体内组成人类脂肪干细胞的三维生物打印结构的成骨分化。
11. Three-dimensional bio-printed constructs consisting of human umbilical-derived mesenchymal stem cells promote cell viability, proliferation, and differentiation in vitro [O] . Qingxia Tao, Cuiying Wu, Xinda Li, 2020

机译：由人脐衍生的间充质干细胞组成的三维生物印刷构建体促进细胞活力，增殖和体外分化
12. Platelet-derived Growth-factor-releasing Aligned Collagen-nanoparticle Fibers Promote the Proliferation and Tenogenic Differentiation of Adipose-derived Stem Cells. [R] . Cheng, X., Tsao, C., Sylvia, V. L., 2013

机译：血小板衍生的生长因子释放对齐胶原 - 纳米粒子纤维促进脂肪来源的干细胞的增殖和tenogenic分化。

Three-dimensional bio-printed constructs consisting of human umbilical-derived mesenchymal stem cells promote cell viability, proliferation, and differentiation in vitro

摘要

著录项

相似文献

相关主题

期刊订阅