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首页> 外文期刊>Journal of Agricultural and Food Chemistry >A Novel beta-1,4-Galactosyltransferase from Histophilus somni Enables Efficient Biosynthesis of Lacto-N-Neotetraose via Both Enzymatic and Cell Factory Approaches
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A Novel beta-1,4-Galactosyltransferase from Histophilus somni Enables Efficient Biosynthesis of Lacto-N-Neotetraose via Both Enzymatic and Cell Factory Approaches

机译：来自组织的新型β-1,4-半乳糖基转移酶通过酶和细胞厂方法可以通过酶和细胞厂方法有效地实现乳酸-N-新生儿

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摘要

Human milk oligosaccharides (HMOs) attract particular attention because of their health benefits for infants. Lacto-N-neotetraose (LNnT) is one of the most abundant neutral core structures of HMOs. Bacterial beta-1,4-galactosyltransferase (beta-1,4-GalT) displays an irreplaceable role in the practical application of LNnT biosynthesis. In this study, a novel beta-1,4-GalT from Histophilus somni was identified to efficiently synthesize LNnT from UDP-Gal and lacto-N-triose II (LNT II). The optimum pH and temperature were determined to be pH 6.0 and 30 degrees C, respectively. The enzyme showed both transgalactosylation and hydrolysis activity, with a specific activity of 3.7 and 6.6 U/mg, respectively. LNnT was synthesized using H. somni beta-1,4-GalT via both enzymatic and cell factory approaches, and both approaches provided an LNnT ratio with the remaining LNT II at approximately 1:2 when reactions attained a balance. These findings indicated that H. somni beta-1,4-GalT has a potential in biosynthesis of LNnT and derivatives in future.
机译：母乳低聚糖（HMO）因其对婴儿健康的益处而受到特别关注。乳糖-N-新四糖（LNnT）是HMO最丰富的中性核心结构之一。细菌β-1,4-半乳糖基转移酶（beta-1,4-GalT）在LNnT生物合成的实际应用中发挥着不可替代的作用。在本研究中，一种来自索马里嗜组织菌的新型β-1,4-半乳糖被鉴定为能有效地从UDP-半乳糖和乳糖-N-三糖II（LNT-II）合成LNnT。最适pH和温度分别为pH 6.0和30℃。该酶具有反半乳糖基化和水解活性，比活性分别为3.7和6.6 U/mg。LNnT是使用H.somniβ-1,4-GalT通过酶法和细胞工厂法合成的，当反应达到平衡时，这两种方法都提供了LNnT与剩余LNT II的比例约为1:2。这些发现表明，索马里H.somniβ-1,4-GalT在未来的LNnT及其衍生物的生物合成中具有潜力。

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