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首页> 外文期刊>Journal of Applied Polymer Science >Gelatin supports with immobilized laccase as sustainable biocatalysts for water treatment
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Gelatin supports with immobilized laccase as sustainable biocatalysts for water treatment

机译：明胶配备固定的漆酶作为可持续的生物催化剂进行水处理

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Millimeter-size beads of gelatin are manufactured by dripping process to give enzyme supports qualified for micropollutants biodegradation in alternative wastewater treatment. The bead diameter is dependent on the tip diameter, the gelatin solution viscosity and the swelling of polymer chains in the collecting bath. Chemical crosslinking was performed with glutaraldehyde using optimal concentration to give mechanical and thermal properties suitable for application in stirred reactor in aqueous medium. Laccases fromTrametes versicolorare grafted on the gelatin beads with glutaraldehyde. Sixty percentage of the initial enzymatic activity, evaluated by the oxidation of 2 '-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt (ABTS) is maintained after 10 successive cycles of reaction. Thermal stability at 60 degrees C of immobilized biocatalysts is improved when compared to free enzymes (45% vs 10% of relative activity after 6 h of incubation). The simplicity of the procedure to form gelatin beads and their properties make them promising bio-based and biodegradable support for enzyme immobilization.
机译：通过滴注工艺制造毫米大小的明胶珠，以提供在替代废水处理中适合微污染物生物降解的酶载体。珠粒直径取决于尖端直径、明胶溶液粘度和收集槽中聚合物链的膨胀。采用最佳浓度的戊二醛进行化学交联，以获得适用于水介质中搅拌反应器的机械和热性能。用戊二醛将云芝漆酶接枝到明胶珠上。通过氧化2’-叠氮基双（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS），在连续10个反应循环后，维持60%的初始酶活性。与游离酶相比，固定化生物催化剂在60℃下的热稳定性有所提高（培养6小时后相对活性的45%对10%）。明胶珠的制备过程简单，性质优良，是一种很有前途的生物基和可生物降解的酶固定化载体。

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