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首页> 外文期刊>Journal of Applied Polymer Science >Fabrication and characterization of chitosan/kefiran electrospun nanofibers for tissue engineering applications
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Fabrication and characterization of chitosan/kefiran electrospun nanofibers for tissue engineering applications

机译：用于组织工程应用的壳聚糖/ Kefiran Electrom纳米纤维的制备与表征

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摘要

Chitosan (CS)-based nanofibrous scaffolds are very promising in tissue engineering applications. However, electrospinning of CS is not possible unless using toxic solvents such as trifluoroacetic acid or by blending with other polymers. In the present study, we investigated CS-based nanofibers' fabrication by blending it with kefiran as a natural polysaccharide. A series of solutions with various CS to kefiran ratios were prepared and underwent electrospinning. The effects of main process parameters, including applied voltage and needle tip-to-collector distance on nanofibers' diameter and morphology, were also studied. Nanofibers containing 80% CS and 20% Kefiran with an average diameter of 81 +/- 17 nm were successfully electrospun. Thermogravimetric analysis indicated the presence of both polymers in blend nanofibers. The diameter of CS/kefiran nanofibers increased with enhanced applied voltage, while needle tip-to-collector distance did not significantly affect the mean diameters. Appropriate viability of l929 cells on the obtained scaffolds was demonstrated utilizing Alamar blue assay. Also, cell attachment onto the fiber surface was confirmed by scanning electron microscopy. Results indicated that CS/kefiran nanofibrous scaffolds would be promising for tissue engineering applications.
机译：基于壳聚糖（CS）的纳米纤维支架在组织工程领域有着广阔的应用前景。然而，除非使用三氟乙酸等有毒溶剂或与其他聚合物混合，否则静电纺丝CS是不可能的。在本研究中，我们研究了CS基纳米纤维的制备方法，将其与天然多糖kefiran混合。制备了一系列具有不同CS与kefiran比率的溶液，并进行了静电纺丝。研究了主要工艺参数，包括外加电压和针尖到集电极的距离对纳米纤维直径和形貌的影响。含有80%CS和20%Kefiran的平均直径为81+/-17nm的纳米纤维被成功电纺。热重分析表明共混纳米纤维中存在这两种聚合物。CS/kefiran纳米纤维的直径随着外加电压的增加而增加，而针尖到集电极的距离对平均直径没有显著影响。利用阿拉玛蓝试验证明l929细胞在获得的支架上具有适当的活性。扫描电子显微镜也证实了细胞附着在纤维表面。结果表明，CS/kefiran纳米纤维支架在组织工程领域具有广阔的应用前景。

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