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首页> 外文期刊>Journal of Applied Polymer Science >An innovative approach to fabricate a thermosensitive melatonin-loaded conductive pluronic/chitosan hydrogel for myocardial tissue engineering
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An innovative approach to fabricate a thermosensitive melatonin-loaded conductive pluronic/chitosan hydrogel for myocardial tissue engineering

机译：制造热敏褪黑素负载型传导性Pluronic / Chotosan水凝胶的创新方法进行心肌组织工程

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摘要

This study aimed at designing and fabrication of a novel injectable and thermosensitive melatonin-loaded pluronic/chitosan hydrogel containing gold nanoparticles (GNPs) and poly glycerol sebacate (PGS) for myocardial tissue engineering. The PGS nanoparticles were used as the melatonin (drug model) carrier. The gelation time, syringeability, stability, and swelling of the hydrogel were scrutinized. Rheological properties, chemical composition, and morphology of the samples were also investigated. The effect of GNPs addition on the electrical conductivity of hydrogel was assessed. The cytotoxicity of hydrogels was assessed through MTT assay in the exposure of H9C2 cells up to 7 days. Scanning electron microscopy was applied to evaluate the morphology of seeded cells. The synthesis parameters of PGS nanoparticles were optimized through which 2.5%w/v of PGS and 1:10 organic phase to aqueous phase (O/A) ratio were found desirable. The optimum hydrogel illustrated 2 min gelation time and was stable up to 20 days with 5% swelling in the first 12 h into phosphate buffered saline. The GNPs with a uniform distribution rendered the hydrogel electrically conductive (1500 mu S/cm). According to the MTT assay results, 3.125 mu M melatonin was considered as the suitable concentration by which a significant increase in the cell viability was observed. The results exhibited that the prepared hydrogel composed of pluronic/chitosan/GNPs, and 3.125 mu M melatonin-loaded PGS nanoparticles could be applied as a promising scaffold for myocardial tissue engineering.
机译：本研究旨在设计和制备用于心肌组织工程的新型可注射、热敏性褪黑素载金纳米颗粒（GNPs）和聚癸二酸甘油酯（PGS）的pluronic/壳聚糖水凝胶。PGS纳米颗粒被用作褪黑素（药物模型）载体。考察了凝胶的凝胶时间、可注射性、稳定性和溶胀性。还研究了样品的流变性能、化学成分和形态。评估了添加GNPs对水凝胶导电性的影响。在H9C2细胞暴露7天后，通过MTT法评估水凝胶的细胞毒性。扫描电子显微镜用于评估种子细胞的形态。优化了PGS纳米颗粒的合成参数，得到了2.5%w/v的PGS和1:10的有机相/水相（O/A）比。最佳水凝胶的凝胶化时间为2分钟，在磷酸盐缓冲盐水中的前12小时内，凝胶稳定达20天，溶胀率为5%。均匀分布的GNP使水凝胶导电（1500μS/cm）。根据MTT试验结果，3.125μM褪黑素被认为是观察到细胞活力显著增加的合适浓度。结果表明，由pluronic/壳聚糖/GNPs和3.125μM载褪黑素PGS纳米粒组成的水凝胶可作为心肌组织工程的支架材料。

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