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首页> 外文期刊>Journal of Applied Polymer Science >Fabrication and characterization of electrospun psyllium husk-based nanofibers for tissue regeneration
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Fabrication and characterization of electrospun psyllium husk-based nanofibers for tissue regeneration

机译：用于组织再生的电纺椎间壳基壳种乳头植物的制造与表征

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The present study reports for first time the blending of psyllium husk (PH) powder/gelatin (G) in the polymer-rich composition of polyvinyl alcohol (PVA) to make an electrospinnable solution. The composite was prepared in 3 different ratios viz., 100% (wt/wt) (PVA + PH), 75% + 25% (PVA + 75PH + 25G) (wt/wt) and 50% + 50% (PVA + 50PH + 50G) (wt/wt) in 6% PVA solution. Optimum electrospinning parameters were evaluated for all the prepared blends. The fabricated nanofibers were characterized by scanning electron microscopy (SEM), attenuated total reflectance-Fourier transform infrared, differential scanning calorimetry, porosity percentage, and fiber orientation using ImageJ software. A qualitative in vitro degradation study at room temperature is supported by SEM images. The cellular interactions were characterized by MTT assay of NIH-3T3 fibroblast cells for 2 and 4 days with an optimum cell growth of >50% by fourth day of culture and long-term cultivation of L929-RFP cells was observed for 10 days. The nanofibers were formed in the range of 49-600 nm. PVA + 75PH + 25G when cultured with L929-RFP cells exhibited highest fluorescence intensity and thus supported cellular proliferation significantly. Based on the results obtained from various analyses, we anticipate that fabricated psyllium-based nanofiber can be used as a promising candidate for wound healing and other biomedical applications.
机译：本研究首次报道了在富含聚合物的聚乙烯醇（PVA）中混合木虱壳（PH）粉末/明胶（G），以制备静电纺丝溶液。以3种不同比例制备复合材料，即：。，在6%PVA溶液中，100%（重量/重量）（PVA+PH）、75%+25%（PVA+75PH+25G）（重量/重量）和50%+50%（PVA+50PH+50G）（重量/重量）。对所有制备的共混物的最佳静电纺丝参数进行了评估。通过扫描电子显微镜（SEM）、衰减全反射傅里叶变换红外光谱、差示扫描量热法、孔隙率和使用ImageJ软件的纤维取向对所制备的纳米纤维进行了表征。SEM图像支持了室温下的定性体外降解研究。通过MTT法对NIH-3T3成纤维细胞进行2天和4天的细胞相互作用分析，在培养的第4天，细胞的最佳生长率>50%，观察L929-RFP细胞的长期培养10天。纳米纤维的形成范围为49-600nm。当PVA+75PH+25G与L929-RFP细胞一起培养时，显示出最高的荧光强度，因此显著支持细胞增殖。基于从各种分析中获得的结果，我们预期所制备的基于木虱的纳米纤维可以用作伤口愈合和其他生物医学应用的有希望的候选者。

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