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首页> 外文期刊>Journal of Applied Polymer Science >Polyester functionalization for mycobacterial capture
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Polyester functionalization for mycobacterial capture

机译：分枝杆菌捕获的聚酯官能化

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Tuberculosis (TB) is one of the deadliest diseases known to man and ranks among the top ten global causes of fatalities. Children often develop a paucibacillary form of this disease which makes diagnosis arduous even with the aid of sophisticated techniques. In these cases, the concentration of bacteria in their sputum falls below the lowest limit of detection for sputum smear microscopy, the technique predominantly used in developing countries. In this study we aim to test the hypothesis that modifying a polymer, often used in buccal swabs, could assist in creating a capturing and concentrating oral swab for Mycobacterium tuberculosis (Mtb), the pathogenic bacterial species responsible for TB. Such a device will assist in meeting the detection limit and allow for rapid detection of the disease. The polymer used was a micro fibrous form of poly(ethylene terephthalate) (PET) which was surface functionalized with Concanavalin A (Con A), a lectin based adhesin with an affinity for the mannose groups on the Mtb cell wall. The functionalization was mediated with glutaraldehyde as bioconjugate molecule and found to produce a surface uniformly covered with Con A. Affinity studies between the modified fibers and the Mycobacterium bovis bacillus Calmette-Guerin (BCG), as Mtb mimic, were conducted to evaluate the capturing abilities of the substrate. The results indicate that the fibers avidly captured BCG and that the fibrous matrix aided in its function. Dilution studies showed successful capturing of the bacterial species at concentrations characteristic of paucibacillary cases. In this study a commercial polymeric material was successfully surface modified with a biological entity to create a substrate to which Mtb could adhere and accordingly be captured.
机译：肺结核（TB）是人类已知的最致命的疾病之一，是全球十大致死原因之一。儿童经常患上这种疾病的贫杆菌型，即使借助复杂的技术，诊断也很困难。在这些病例中，他们痰中的细菌浓度低于痰涂片显微镜检测的最低限度，这项技术主要用于发展中国家。在这项研究中，我们的目的是验证一个假设，即修饰一种常用于口腔拭子的聚合物，可以帮助创建一种捕获和浓缩结核分枝杆菌（Mtb）的口腔拭子，结核分枝杆菌是导致结核的致病细菌。这种设备将有助于达到检测极限，并允许快速检测疾病。所使用的聚合物是聚对苯二甲酸乙二醇酯（PET）的微纤维形式，其表面通过刀豆球蛋白a（Con a）功能化，刀豆球蛋白a是一种基于凝集素的粘附素，与Mtb细胞壁上的甘露糖基团具有亲和力。通过戊二醛作为生物结合分子介导功能化，发现其表面均匀覆盖有Con a。进行了改性纤维与作为Mtb模拟物的牛分枝杆菌-卡介苗（BCG）之间的亲和力研究，以评估底物的捕获能力。结果表明，纤维能贪婪地捕获卡介苗，纤维基质有助于卡介苗的功能。稀释研究表明，在具有贫杆菌病例特征的浓度下，成功捕获了细菌物种。在这项研究中，一种商用聚合物材料成功地用生物实体进行表面改性，以创建一种基质，Mtb可以粘附在该基质上，并因此被捕获。

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