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首页> 外文期刊>Journal of Applied Polymer Science >Evaluating the protective role of carrier microparticles in preserving protein secondary structure within electrospun meshes
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Evaluating the protective role of carrier microparticles in preserving protein secondary structure within electrospun meshes

机译：评估载体微粒在防纺网内保存蛋白质二级结构的保护作用

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Direct incorporation of proteins into electrospun meshes using approaches such as blend electrospinning can promote adverse interactions with hydrophobic polymers, organic solvents and high voltage, potentially leading to loss of protein activity. However, pre-encapsulation within a protective carrier phase can preserve protein conformation by avoiding exposure to harsh processing conditions. In this study, bovine serum albumin (BSA) was loaded within cellulose microparticles (MPs) and the BSA-loaded MPs were dispersed in a solution of poly(ethylene oxide) (PEO). Particle-mesh composites were created using a sacrificial fiber/co-electrospinning approach in which the BSA/MP/PEO solution was simultaneously electrospun against a poly(caprolactone) (PCL) solution. Post-fabrication, sacrificial PEO fibers were selectively dissolved by treatment with ethanol. Microscopy, weight loss analysis and FTIR spectroscopy together confirmed selective dissolution of PEO fibers and the retention of BSA-loaded MPs within the PCL network without significant loss of either the MPs or the protein. Circular dichroism spectroscopy and intrinsic fluorescence measurements on BSA extracted from the co-electrospun meshes indicated minimal disruption to secondary structure, although partial sheet induction was observed. In contrast, direct exposure of BSA to four commonly used electrospinning solvents resulted in a large decrease in helical content and significant induction of sheets, revealing significant changes to the secondary structure. In summary, our results demonstrate the protective role of MPs in minimizing adverse effects of electrospinning on the secondary structure of incorporated protein.
机译：使用混合静电纺丝等方法将蛋白质直接并入静电纺丝网中，可促进与疏水聚合物、有机溶剂和高压的不利相互作用，可能导致蛋白质活性的丧失。然而，保护性载体相内的预包封可以通过避免暴露于苛刻的加工条件来保持蛋白质构象。在这项研究中，牛血清白蛋白（BSA）被装载在纤维素微粒（MPs）中，并且装载BSA的MPs被分散在聚环氧乙烷（PEO）溶液中。使用牺牲纤维/共静电纺丝方法制造颗粒网状复合材料，其中BSA/MP/PEO溶液与聚己内酯（PCL）溶液同时静电纺丝。制作后，通过乙醇处理选择性溶解牺牲PEO纤维。显微镜、失重分析和FTIR光谱共同证实了PEO纤维的选择性溶解，以及负载BSA的MPs在PCL网络中的保留，而MPs或蛋白质没有显著损失。圆二色光谱和本征荧光测量表明，尽管观察到部分片状诱导，但从共静电纺丝网中提取的BSA对二级结构的破坏最小。相比之下，将BSA直接暴露于四种常用的静电纺丝溶剂中，导致螺旋含量大幅降低，并显著诱导片材形成，揭示了二级结构的显著变化。总之，我们的结果证明了多磺酸粘多糖在最小化静电纺丝对蛋白质二级结构的不利影响方面的保护作用。

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