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首页> 外文期刊>Journal of Applied Polymer Science >Polymer brush-grafted monolithic macroporous polyHIPEs obtained by surface-initiated ARGET ATRP and heparinized for Enterovirus 71 purification
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Polymer brush-grafted monolithic macroporous polyHIPEs obtained by surface-initiated ARGET ATRP and heparinized for Enterovirus 71 purification

机译：通过表面引发的arget ATRP获得的聚合物刷嫁接整体式聚合物，并为肠道病毒71纯化纯化

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摘要

Monolithic macroporous polymerized high internal phase emulsions (polyHIPEs) were grafted with poly(glycidyl methacrylate) (PGMA) brushes via surface-initiated activators continuously regenerated by electron transfer atom transfer radical polymerization (ARGET ATRP) and heparinized for obtaining an affinity chromatographic column for Enterovirus 71 (EV71) purification. The proposed strategy possessed of three advantages. (a) Highly interconnected porous structure of polyHIPEs guaranteed an excellent permeability (9.3 x 10(-14) m(2)) and fast mass transfer of biomacromolecules; (b) PGMA brushes provided plentiful epoxy groups for heparin immobilization; (c) High accessibility of heparin was achieved as the polymer brushes could extend into the interior of pores. A specific capture of EV71 was accomplished by receptor-ligand interaction. Dynamic adsorption capacity for EV71 reached 768 ng per column, up to 5-fold higher than nongrafted one reported in our previous work. The maximum recovery for EV71 was 76.1%. Outstanding adsorption performances were attributed to the high density and accessibility of heparin immobilized on PGMA brushes. In general, the developed polymer brush-grafted polyHIPEs opened up a new broad road for design of novel chromatographic medium for bioseparation and biomedical applications.
机译：通过电子转移原子转移自由基聚合（ARGET ATRP）连续再生的表面引发活化剂，用聚甲基丙烯酸缩水甘油酯（PGMA）刷接枝整体大孔聚合高内相乳液（polyHIPEs），并肝素化，获得用于纯化肠病毒71型（EV71）的亲和色谱柱。提出的战略有三个优点。（a） 高度互连的多孔结构保证了生物大分子的良好渗透性（9.3x10（-14）m2））和快速传质；（b） PGMA刷为肝素固定提供了丰富的环氧基团；（c） 由于聚合物刷可以延伸到毛孔内部，因此肝素的可及性很高。EV71的特异性捕获是通过受体-配体相互作用实现的。EV71的动态吸附容量达到768纳克/柱，比我们之前工作中报告的非嫁接柱高出5倍。EV71的最大回收率为76.1%。优异的吸附性能归因于固定在PGMA刷上的肝素的高密度和可接近性。总的来说，所开发的聚合物刷接枝聚合物芯片为设计用于生物分离和生物医学应用的新型色谱介质开辟了新的广阔道路。

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