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首页> 外文期刊>Journal of chromatography, A: Including electrophoresis and other separation methods >A simple and highly efficient counter-current chromatography method for the isolation of concentrated fractions of compounds based on the sequential sample loading technique: Comparative theoretical study of conventional multiple and intermittent sample loading counter-current chromatography separations
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A simple and highly efficient counter-current chromatography method for the isolation of concentrated fractions of compounds based on the sequential sample loading technique: Comparative theoretical study of conventional multiple and intermittent sample loading counter-current chromatography separations

机译：一种简单且高效的逆流色谱法，用于基于顺序样品加载技术分离化合物的浓缩级分：常规多发性样品加载逆流色谱分离的比较理论研究

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摘要

A new modification of the conventional multiple sample loading (MSL) mode - sequential sample load-ing (SSL) - is suggested to enhance further the performance of the counter-current chromatography (CCC) separation processes. The sequential sample loading technique is simple and easy to implement: the con-tinuous sample solution supply to a CCC column is alternated (interrupted) with short periods of the "pure" mobile phase supply. Periodic (batch) and continuous SSL CCC separations can be designed and implemented. In continuous processes, the sample solution loading is carried out in the form of sepa-rate series, consisting of a number of sequential sample solution loads. In this work, the modeling of the conventional multiple sample loading and the sequential sample loading counter-current chromatography is used to compare the two operating modes considered. Equations for the calculation of band profiles, the recovery yield and the purity are given. Equations are also derived permitting the calculation of the optimum operating parameters of the separation processes. It is shown that the use of sequential sample loading makes it possible to produce fractions of purified compounds with a much higher concentration than in the original sample solution. The simulations of the conventional multiple sample loading and the sequential sample loading counter-current chromatography separations are presented in "Mathcad" software. (c) 2021 Elsevier B.V. All rights reserved.
机译：为了进一步提高逆流色谱（CCC）分离过程的性能，建议对传统的多样本加载（MSL）模式进行新的修改——顺序样本加载（SSL）。顺序进样技术简单且易于实现：连续向CCC柱供应样品溶液与短时间的“纯”流动相交替（中断）。可以设计和实施定期（批量）和连续的SSL CCC分离。在连续过程中，样品溶液加载以分离率序列的形式进行，由多个连续样品溶液加载组成。在这项工作中，传统的多样品加载和顺序样品加载逆流色谱的建模被用来比较这两种操作模式。给出了谱带分布、回收率和纯度的计算公式。还导出了允许计算分离过程最佳操作参数的方程式。结果表明，使用顺序进样可以产生比原始样品溶液浓度高得多的纯化化合物。“Mathcad”软件对传统的多样本加载和顺序样本加载逆流色谱分离进行了模拟。（c）2021爱思唯尔B.V.保留所有权利。

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