interferENZY: A Web-Based Tool for Enzymatic Assay Validation and Standardized Kinetic Analysis

Pinto Maria Filipa; Baici Antonio; Barbosa Pereira Pedro Jose; Macedo-Ribeiro Sandra; Pastore Annalisa; Rocha Fernando; Martins Pedro M.

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interferENZY: A Web-Based Tool for Enzymatic Assay Validation and Standardized Kinetic Analysis

机译：细胞细胞：一种基于Web的酶测定验证和标准化动力学分析的工具

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Enzymatic assays are widely employed to characterize important allosteric and enzyme modulation effects. The high sensitivity of these assays can represent a serious problem if the occurrence of experimental errors surreptitiously affects the reliability of enzyme kinetics results. We have addressed this problem and found that hidden assay interferences can be unveiled by the graphical representation of progress curves in modified reaction coordinates. To render this analysis accessible to users across all levels of expertise, we have developed a webserver, interferENZY, that allows (i) an unprecedented tight quality control of experimental data, (ii) the automated identification of small and major assay interferences, and (iii) the estimation of bias-free kinetic parameters. By eliminating the subjectivity factor in kinetic data reporting, interferENZY will contribute to solving the "reproducibility crisis" that currently challenges experimental molecular biology. (C) 2020 Elsevier Ltd. All rights reserved.

机译：酶分析被广泛用于表征重要的变构和酶调节效应。如果实验错误的发生偷偷地影响酶动力学结果的可靠性，那么这些分析的高灵敏度可能是一个严重的问题。我们已经解决了这个问题，并发现隐藏的分析干扰可以通过修改反应坐标中进度曲线的图形表示来揭示。为了让所有专业水平的用户都能访问该分析，我们开发了一个名为interferENZY的网络服务器，它允许（i）对实验数据进行前所未有的严格质量控制，（ii）自动识别小的和主要的分析干扰，以及（iii）估计无偏差的动力学参数。通过消除动力学数据报告中的主观性因素，干扰将有助于解决目前挑战实验分子生物学的“再现性危机”。（C） 2020爱思唯尔有限公司版权所有。

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来源
《Journal of Molecular Biology》 |2021年第11期|共9页
作者
Pinto Maria Filipa; Baici Antonio; Barbosa Pereira Pedro Jose; Macedo-Ribeiro Sandra; Pastore Annalisa; Rocha Fernando; Martins Pedro M.;
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作者单位

Univ Porto ICBAS Inst Ciencias Biomed Abel Salazar Rua Jorge Viterbo Ferreira 228 P-4050313;

Univ Zurich Dept Biochem Winterthurerstr 190 CH-8057 Zurich Switzerland;

Univ Porto i3S Inst Invest &

Inovacao Saude Rua Alfredo Allen 208 P-4200135 Porto Portugal;

Univ Porto i3S Inst Invest &

Inovacao Saude Rua Alfredo Allen 208 P-4200135 Porto Portugal;

Kings Coll London Maurice Wohl Clin Neurosci Inst 5 Cutcombe Rd London SE5 9RT England;

Univ Porto LEPABE Lab Engn Proc Ambiente Biotecnol &

Energia Dept Engn Quim Fac Engn Rua Dr;

Univ Porto ICBAS Inst Ciencias Biomed Abel Salazar Rua Jorge Viterbo Ferreira 228 P-4050313;

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原文格式 PDF
正文语种 eng
中图分类分子生物学;
关键词
enzyme kinetics; webserver; data pollution; linearization method; reproducibility;

机译：酶动力学;网络服务器;数据污染;线性化方法;再现性;

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interferENZY: A Web-Based Tool for Enzymatic Assay Validation and Standardized Kinetic Analysis

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