...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Physics, D. Applied Physics: A Europhysics Journal >Quantitative detection of optical anisotropy of single microtubules by polarization-sensitive interferometric scattering microscopy
【24h】

Quantitative detection of optical anisotropy of single microtubules by polarization-sensitive interferometric scattering microscopy

机译：通过极化敏感干涉散射显微镜定量检测单微管的光学各向异性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Microtubules (MTs) are ubiquitous cytoskeletal biopolymers essential for diverse cellular processes. MTs consist of strictly ordered tubulin dimers arranged into hollow cylindrical filaments and are known to be optically anisotropic, which enables their direct observation in microscopes based on polarization contrast. However, there are no experimental data to quantify the relation between the momentary optical anisotropy of the MT and the immediate arrangement of proteins in the MT structure. In this work, we introduce polarization-sensitive microscopy based on interferometric detection of scattering to quantify the scattering anisotropy of single unlabeled MTs with high precision. Our data explain the structural origin of MT anisotropy with a marginal contribution of the intrinsic asymmetry of a single tubulin molecule. We monitor changes in the MT scattering resulting from the binding of tau proteins to single MTs with a resolution of several proteins per diffraction-limited spot. We associate the changes in the contrast as well as in the scattering anisotropy with the formation of a shell around the MT formed by densely packed tau proteins. Our experimental results match closely with the theoretical model of the MT and include quantitative details about the polarization-dependent interaction of light with biological matter.
机译：微管（MTs）是广泛存在的细胞骨架生物聚合物，对多种细胞过程至关重要。MTs由排列成中空圆柱形细丝的严格有序的微管蛋白二聚体组成，已知具有光学各向异性，这使得它们能够在显微镜下根据偏振对比度进行直接观察。然而，没有实验数据来量化MT的瞬时光学各向异性与MT结构中蛋白质的直接排列之间的关系。在这项工作中，我们引入了基于散射干涉检测的偏振敏感显微镜，以高精度量化单个未标记MTs的散射各向异性。我们的数据解释了MT各向异性的结构起源，单个微管蛋白分子的内在不对称性起到了边际作用。我们监测由于tau蛋白与单个MTs结合而导致的MT散射的变化，每个衍射限制点的分辨率为几个蛋白质。我们将对比度和散射各向异性的变化与由密集的tau蛋白形成的MT周围的壳层的形成联系起来。我们的实验结果与MT的理论模型非常吻合，并且包含了光与生物物质偏振相关相互作用的定量细节。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号