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首页> 外文期刊>Cancer biology & therapy >miR-219a-1 inhibits colon cancer cells proliferation and invasion by targeting MEMO1
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miR-219a-1 inhibits colon cancer cells proliferation and invasion by targeting MEMO1

机译：miR-219a-1通过靶向备忘录抑制结肠癌细胞的增殖和侵袭

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摘要

Colon cancer is the third most common cancer worldwide. Many miRNAs have been reported to be involved in colon cancer progression. However, there are only a few studies on the role of miR-219a-1 in colon cancer, and the molecular mechanisms involved remain unclear. The aim of this study was to investigate the miR-219a-1 level in patients with colon cancer and to explore both the effects and regulatory mechanisms of miR-219a-1 in the malignancy of colon cancer cells. Real-time PCR and western blot analysis were used to analyze the expression levels of miR-219a-1 and mediator of ErbB2-driven cell motility 1. Cell Counting Kit-8, transwell and wound-healing assays were performed to investigate the malignant ability of colon cancer cells. A luciferase assay was performed to explore whether miR-219a-1 could directly bind to 3MODIFIER LETTER PRIME-UTR region of MEMO1. miR-219a-1 was found to be downregulated in colon cancer cell lines and in patients with colon cancer. Additionally, miR-219a-1 could inhibit colon cancer cell proliferation, invasion and migration. We identified MEMO1 as a novel potential target gene of miR-219a-1. Luciferase assays showed that miR-219a-1 could directly bind to 3 '-UTR of MEMO1. Overexpression of miR-219a-1 in colon cancer cells could inhibit the expression of MEMO1. Furthermore, MEMO1 was upregulated in patients with colon cancer, which was inversely correlated with miR-219a-1 levels. In conclusion, our study revealed that miR-219a-1 exerts anti-tumor effects and regulates colon cancer cell proliferation, invasion and migration by targeting MEMO1, suggesting that miR-219a-1 could act as a therapeutic target in colon cancer.
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  • 来源
    《Cancer biology & therapy》 |2020年第12期|共8页
  • 作者

    Xu Keqing; Shi Jie; Mo Dongping; Yang Yanhua; Fu Qiang; Luo Ying;

  • 作者单位

    East China Normal Univ Changning Matern &

    Infant Hlth Hosp Dept Clin Lab Shanghai Peoples R;

    Changzhou 7 Peoples Hosp Nanjing Red Cross Blood Ctr Dept Comprehens Med Lab Changzhou Peoples;

    Nanjing Red Cross Blood Ctr Nanjing Peoples R China;

    East China Normal Univ Changning Matern &

    Infant Hlth Hosp Dept Clin Lab Shanghai Peoples R;

    Changzhou 7 Peoples Hosp Nanjing Red Cross Blood Ctr Dept Comprehens Med Lab Changzhou Peoples;

    Nanjing Med Univ Dept Clin Lab Jiangsu Canc Hosp Nanjing Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 肿瘤学;
  • 关键词

    Mir-219a-1; colon cancer cells; proliferation; invasion; migration;

    机译：miR-219a-1;结肠癌细胞;增殖;入侵;迁移;

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