miR-221-3p promotes hepatocellular carcinogenesis by downregulating O6-methylguanine-DNA methyltransferase

Chen Zushun; Xiang Bangde; Qi Lunan; Zhu Shaoliang; Li Lequn

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miR-221-3p promotes hepatocellular carcinogenesis by downregulating O6-methylguanine-DNA methyltransferase

机译：MiR-221-3P通过下调O6-甲基胍-DNA甲基转移酶来促进肝细胞癌

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This study aimed to investigate the influence of miR-221-3p and O-6-methylguanine-DNA methyltransferase (MGMT) interaction in human hepatocellular carcinoma (HCC), thereby revealing a novel molecular mechanism of hepatic carcinogenesis involving miR-221-3p and MGMT. Fluorescence qPCR and immunoblot assays were performed to determine the expression of RNA and protein in HCC tissues and cell lines. We also employed the firefly and Renilla luciferase assay to verify the target relationship between miR-221-3p and MGMT mRNA. Assessments including the MTT assay, wound-healing assay, transwell assay, colony foci formation experiment, and flow cytometric experiment were carried out to determine the viability, migration, invasion, proliferation, cell cycle progression, and apoptosis of SMMC-7721 and BEL-7404 cell lines with the modulated expression of miR-221-3p and MGMT. Compared to healthy tissues and cell line HL7702, miR-221-3p was significantly upregulated but MGMT was significantly downregulated in carcinomas and cancerous cell lines. Forced miR-221-3p overexpression was found to enhance the proliferation, migration, invasion, and clonogenicity of cell lines, but it suppressed cell apoptosis. Findings also revealed that forced miR-221-3p overexpression had little effect on cell cycle progression. After MGMT was confirmed to be atarget gene of miR-221-3p, it was found that the forced upregulation of miR-221-3p downregulated MGMT mRNA and protein levels significantly. MiR-221-3p was identified as an HCC promoting factor, and it specifically inhibited the expression of the MGMT. Besides, the upregulation of miR-221-3p had apositive influence on HCC pathogenesis by inhibiting MGMT expression.

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来源
《Cancer biology & therapy》 |2020年第12期|共12页
作者
Chen Zushun; Xiang Bangde; Qi Lunan; Zhu Shaoliang; Li Lequn;
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作者单位

Guangxi Med Univ Dept Hepatobiliary Surg Affiliated Tumor Hosp Nanning 530021 Guangxi Peoples;

Guangxi Med Univ Dept Hepatobiliary Surg Affiliated Tumor Hosp Nanning 530021 Guangxi Peoples;

Guangxi Med Univ Dept Hepatobiliary Surg Affiliated Tumor Hosp Nanning 530021 Guangxi Peoples;

Guangxi Med Univ Dept Hepatobiliary Surg Affiliated Tumor Hosp Nanning 530021 Guangxi Peoples;

Guangxi Med Univ Dept Hepatobiliary Surg Affiliated Tumor Hosp Nanning 530021 Guangxi Peoples;

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原文格式 PDF
正文语种 eng
中图分类肿瘤学;
关键词
miR-221-3p; MGMT; hepatocellular carcinoma;

机译：miR-221-3p;mgmt;肝细胞癌;

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机译：熊果酸通过下调O6-甲基鸟嘌呤-DNA甲基转移酶（MGMT）的表达减弱胶质母细胞瘤细胞中的替莫唑胺耐药性
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机译：碱基切除修复和O6-甲基鸟嘌呤-DNA甲基转移酶均能防止甲基化诱导的结肠癌发生
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机译：碱基切除修复和O6-甲基鸟嘌呤-DNA甲基转移酶均可以防止甲基化诱导的结肠癌发生。
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10. Ursolic acid attenuates temozolomide resistance in glioblastoma cells by downregulating O6-methylguanine-DNA methyltransferase (MGMT) expression [O] . Zhongling Zhu, Shuangshuang Du, Fengxia Ding, 2016

机译：熊果酸通过下调O6-甲基鸟嘌呤-DNA甲基转移酶（MGMT）的表达减弱胶质母细胞瘤细胞中的替莫唑胺抗性
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12. Nonapoptotic Function of Caspase-6 in Promoting Mammary Carcinogenesis. [R] . Xu, X. 2012

机译：Caspase-6促进乳腺癌发生的非凋亡作用。

miR-221-3p promotes hepatocellular carcinogenesis by downregulating O6-methylguanine-DNA methyltransferase

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