• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta biomaterialia >Stable subcutaneous cartilage regeneration of bone marrow stromal cells directed by chondrocyte sheet
【24h】

Stable subcutaneous cartilage regeneration of bone marrow stromal cells directed by chondrocyte sheet

机译：软骨细胞板指导的骨髓基质细胞的稳定皮下软骨再生

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In vivo niche plays an important role in regulating differentiation fate of stem cells. Due to lack of proper chondrogenic niche, stable cartilage regeneration of bone marrow stromal cells (BMSCs) in subcutaneous environments is always a great challenge. This study explored the feasibility that chondrocyte sheet created chondrogenic niche retained chondrogenic phenotype of BMSC engineered cartilage (BEC) in subcutaneous environments. Porcine BMSCs were seeded into biodegradable scaffolds followed by 4 weeks of chondrogenic induction in vitro to form BEC, which were wrapped with chondrocyte sheets (Sheet group), acellular small intestinal submucosa (SIS, SIS group), or nothing (Blank group) respectively and then implanted subcutaneously into nude mice to trace the maintenance of chondrogenic phenotype. The results showed that all the constructs in Sheet group displayed typical cartilaginous features with abundant lacunae and cartilage specific matrices deposition. These samples became more mature with prolonged in vivo implantation, and few signs of ossification were observed at all time points except for one sample that had not been wrapped completely. Cell labeling results in Sheet group further revealed that the implanted BEC directly participated in cartilage formation. Samples in both SIS and Blank groups mainly showed ossified tissue at all time points with partial fibrogenesis in a few samples. These results suggested that chondrocyte sheet could create a chondrogenic niche for retaining chondrogenic phenotype of BEC in subcutaneous environment and thus provide a novel research model for stable ectopic cartilage regeneration based on stem cells.
机译：None

著录项

  • 来源
    《Acta biomaterialia》 |2017年第2017期|共12页
  • 作者

    Li Dan; Zhu Lian; Liu Yu; Yin Zongqi; Liu Yi; Liu Fangjun; He Aijuan; Feng Shaoqing; Zhang Yixin; Zhang Zhiyong; Zhang Wenjie; Liu Wei; Cao Yilin; Zhou Guangdong;

  • 作者单位

    Shanghai Jiao Tong Univ Shanghai Peoples Hosp 9 Dept Plast &

    Reconstruct Surg Shanghai Key Lab;

    Shanghai Jiao Tong Univ Shanghai Peoples Hosp 9 Dept Plast &

    Reconstruct Surg Shanghai Key Lab;

    Natl Tissue Engn Ctr China Shanghai Peoples R China;

    Shanghai Jiao Tong Univ Shanghai Peoples Hosp 9 Dept Plast &

    Reconstruct Surg Shanghai Key Lab;

    Chinese Acad Med Sci Inst Dermatol Nanjing Jiangsu Peoples R China;

    Wei Fang Med Coll Plast Surg Hosp Res Inst Plast Surg Weifang Shandong Peoples R China;

    Shanghai Jiao Tong Univ Shanghai Peoples Hosp 9 Dept Plast &

    Reconstruct Surg Shanghai Key Lab;

    Shanghai Jiao Tong Univ Shanghai Peoples Hosp 9 Dept Plast &

    Reconstruct Surg Shanghai Key Lab;

    Shanghai Jiao Tong Univ Shanghai Peoples Hosp 9 Dept Plast &

    Reconstruct Surg Shanghai Key Lab;

    Shanghai Jiao Tong Univ Shanghai Peoples Hosp 9 Dept Plast &

    Reconstruct Surg Shanghai Key Lab;

    Shanghai Jiao Tong Univ Shanghai Peoples Hosp 9 Dept Plast &

    Reconstruct Surg Shanghai Key Lab;

    Shanghai Jiao Tong Univ Shanghai Peoples Hosp 9 Dept Plast &

    Reconstruct Surg Shanghai Key Lab;

    Shanghai Jiao Tong Univ Shanghai Peoples Hosp 9 Dept Plast &

    Reconstruct Surg Shanghai Key Lab;

    Shanghai Jiao Tong Univ Shanghai Peoples Hosp 9 Dept Plast &

    Reconstruct Surg Shanghai Key Lab;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 普通生物学;
  • 关键词

    BMSCs; Subcutaneous cartilage regeneration; Chondrocyte sheet; Chondrogenic niche; Ossification;

    机译：BMSCS;皮下软骨再生;软骨细胞片;软骨生育乳房;骨化;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号