Puerarin facilitates osteogenesis in steroid-induced necrosis of rabbit femoral head and osteogenesis of steroid-induced osteocytes via miR-34a upregulation

Jiang Xin; Chen Wenjing; Su Hang; Shen Fuguo; Xiao Wenlong; Sun Wencai

首页> 外文期刊>Cytokine >Puerarin facilitates osteogenesis in steroid-induced necrosis of rabbit femoral head and osteogenesis of steroid-induced osteocytes via miR-34a upregulation

【24h】

Puerarin facilitates osteogenesis in steroid-induced necrosis of rabbit femoral head and osteogenesis of steroid-induced osteocytes via miR-34a upregulation

机译：葛根素促进了类固醇诱导的兔股骨头坏死的骨质发生和通过miR-34a上调的类固醇诱导的骨细胞的骨质发生

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The present study investigated the effect of puerarin on promoting the osteogenesis in steroid-induced necrosis of the femoral head (SONFH). New Zealand rabbits were administrated with horse serum and methylprednisolone (MPS) for establishing SONFH in vivo model, which was then treated with puerarin treatment. Histomorphological changes in the femoral head were examined by hematoxylin-eosin staining. Osteoblasts were isolated from healthy rabbits and treated by individual or combined administration of dexamethasone and puerarin. Osteoblast viability was measured by CCK-8 assay. Mineralized nodule formation was evaluated by alizarin red assay. Expressions of RUNX family transcription factor 2 (RUNX2), Type-I collagen alpha 1 (COL1A1), ALP and miR-34a in the femoral head were determined by qRT-PCR and Western blot. Puerarin attenuated the effect of SONFH on promoting histopathological abnormalities and counteracted SONFH inhibition on the expressions of ALP, RUNX2, COL1A1 and miR-34a in the rabbits. Rabbit osteoblasts were successfully isolated, as they showed red mineralized nodules. Dexamethasone exposure decreased osteoblast viability, which was increased by puerarin treatment. Furthermore, puerarin treatment attenuated dexamethasone-induced inhibition on the viability, osteoblastic differentiation, and the expressions of ALP, RUNX2, COL1A1 and miR-34a in the osteoblasts. Puerarin facilitated osteogenesis of steroid-induced necrosis of rabbit femoral head and osteogenesis of steroid-induced osteocytes via miR-34a upregulation.

机译：None

著录项

来源
《Cytokine》 |2021年第1期|共7页
作者
Jiang Xin; Chen Wenjing; Su Hang; Shen Fuguo; Xiao Wenlong; Sun Wencai;
展开▼
作者单位

Qiqihar Med Univ Dept 5 Orthoped Surg Affiliated Hosp 3 27 Taishun St Qiqihar 161099;

Southern Med Univ Qiqihar Hosp Hosp Qiqihar 1 Dept Pathol Qiqihar Heilongjiang Peoples R China;

Qiqihar Med Univ Dept 5 Orthoped Surg Affiliated Hosp 3 27 Taishun St Qiqihar 161099;

Qiqihar Med Univ Dept 5 Orthoped Surg Affiliated Hosp 3 27 Taishun St Qiqihar 161099;

Qiqihar Med Univ Dept 5 Orthoped Surg Affiliated Hosp 3 27 Taishun St Qiqihar 161099;

Qiqihar Med Univ Dept 5 Orthoped Surg Affiliated Hosp 3 27 Taishun St Qiqihar 161099;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类细胞生物学;
关键词
Puerarin; Steroid-induced necrosis of the femoral head; Osteogenesis; Osteoblasts; miR-34a;

机译：葛根素;类固醇诱导的股骨头坏死;成骨;成骨细胞;mir-34a;

相似文献

外文文献
中文文献
专利

1. Experimental study of changes of TNF-α in rabbits with steroid-induced avascular necrosis of femoral head [J] . Puwei Yuan, Xijing He, Dong Wang, 生物医学研究杂志（英文版） . 2005,第003期
2. Effects of Huogu I Formula （活骨I方） on Correlated Factors of Bone Regeneration in Chickens with Steroid-Induced Necrosis of Femoral Head [J] . 陈卫衡, 孔祥英, 万蓉, 中国结合医学杂志：英文版 . 2012,第005期
3. The changes of nitric oxide and hemorheology in steroid-induced avascular necrosis of femoral head [J] . WANG Guo-yu, HE Xi-jing, LI Yi, 中国人民解放军军医大学学报（英文版） . 2006,第002期
4. Silencing MicroRNA-137-3p, which Targets RUNX2 and CXCL12 Prevents Steroid-induced Osteonecrosis of the Femoral Head by Facilitating Osteogenesis and Angiogenesis [J] . Lingchi Kong, Rongtai Zuo, Mengwei Wang, International journal of biological sciences . 2020,第4期

机译：沉默的microRNA-137-3P，靶向runx2和CXCL12通过促进成骨和血管生成来防止股骨头的类固醇诱导的骨膜骨折
5. Oxidative Stress Induced Osteocyte Apoptosis in Steroid-Induced Femoral Head Necrosis [J] . Zhen-Qi Fan, Shu-Cai Bai, Qian Xu MM, Orthopaedic surgery . 2021,第7期

机译：氧化应激诱导骨细胞凋亡在类固醇诱导的股骨头坏死中
6. MiR-20b promotes osteocyte apoptosis in rats with steroid-induced necrosis of the femoral head through BMP signaling pathway [J] . G.-Q. Li, Z.-Y. Wang European review for medical and pharmacological sciences. . 2019,第11期

机译：MiR-20b通过BMP信号通路促进类固醇诱导的股骨头坏死大鼠骨细胞凋亡
7. The effect of serum containing Tao Hong Si Wu decoction on macrophages in steroid-induced osteonecrosis of femoral head [C] . Chen Peng, Fang Bin, He Wei, 2012 IEEE International Conference on Bioinformatics and Biomedicine Workshops. . 2012

机译：桃红四物汤血清对激素引起的股骨头坏死巨噬细胞的影响
8. Investigating the Role of Osteocytes in Osteogenesis Imperfecta [D] . Zimmerman, Sarah M. 2018

机译：调查骨细胞在成骨不全中的作用
9. Silencing MicroRNA-137-3p which Targets RUNX2 and CXCL12 Prevents Steroid-induced Osteonecrosis of the Femoral Head by Facilitating Osteogenesis and Angiogenesis [O] . Lingchi Kong, Rongtai Zuo, Mengwei Wang, 2020

机译：沉默的MicroRNA-137-3p针对RUNX2和CXCL12通过促进成骨和血管生成防止类固醇激素引起的股骨头坏死
10. Sildenafil improves blood perfusion in steroid-induced avascular necrosis of femoral head in rabbits via a protein kinase G-dependent mechanism [O] . Qichun Song, Jianlong Ni, Hongyuan Jiang, 2017

机译：西地那非通过蛋白激酶G依赖机制改善激素诱导的兔股骨头缺血性坏死的血液灌注
11. Evaluation of Femoral Head Necrosis Using a Volumetric Method Based on MRI. [R] . Bassounas, A., Fotiadis, D. I., Malizos, K. N. 2001

机译：基于mRI的容积法评价股骨头坏死。

Puerarin facilitates osteogenesis in steroid-induced necrosis of rabbit femoral head and osteogenesis of steroid-induced osteocytes via miR-34a upregulation

摘要

著录项

相似文献

相关主题

期刊订阅